tetano
Editor, Senior Moderator
Appl Environ Microbiol. 2011 May 27. [Epub ahead of print]
Persistence of avian influenza viruses in lake sediment, duck feces, and duck meat.
Nazir J, Haumacher R, Ike AC, Marschang RE.
Source
Institute of Environmental and Animal Hygiene, University of Hohenheim, Garbenstrasse 30, 70599 Stuttgart, Germany.
Abstract
The persistence of 3 low pathogenicity avian influenza (LPAI) viruses (H4N6, H5N1, and H6N8), one human influenza virus (H1N1) as well as NDV and ECBO was investigated in lake sediment, duck feces, and duck meat at 30, 20, 10, and 0 ?C using a germ carrier technique. Virus loaded germ carriers were incubated in each substrate and residual infectivity of the eluted virus was quantified on cell culture after regular intervals for a maximum of 24 weeks. Data was analyzed by linear regression model to calculate T-90 values and estimated persistence of the viruses. In general, the persistence of all of the viruses was highest in lake sediment followed by in the feces and the shortest in duck meat at all temperatures. For the AIV subtypes, T-90 values in sediment ranged from 5-11, 13-18, 43-54, and 66-394 days at 30, 20, 10, and 0 ?C, respectively, which were 2-5 times higher than the T-90 values of the viruses in the feces and meat. Although the individual viruses vary in their tenacity, the survival time of influenza viruses was comparatively shorter than that of NDV and ECBO in all substrates. The results of this study suggest that lake sediment may act as a long-term source of influenza viruses in the aquatic habitat while the viruses may remain infectious for extended periods of time in duck feces and meat at low temperatures, allowing persistence of the viruses in the environment over winter.
PMID:
21622783
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21622783
Persistence of avian influenza viruses in lake sediment, duck feces, and duck meat.
Nazir J, Haumacher R, Ike AC, Marschang RE.
Source
Institute of Environmental and Animal Hygiene, University of Hohenheim, Garbenstrasse 30, 70599 Stuttgart, Germany.
Abstract
The persistence of 3 low pathogenicity avian influenza (LPAI) viruses (H4N6, H5N1, and H6N8), one human influenza virus (H1N1) as well as NDV and ECBO was investigated in lake sediment, duck feces, and duck meat at 30, 20, 10, and 0 ?C using a germ carrier technique. Virus loaded germ carriers were incubated in each substrate and residual infectivity of the eluted virus was quantified on cell culture after regular intervals for a maximum of 24 weeks. Data was analyzed by linear regression model to calculate T-90 values and estimated persistence of the viruses. In general, the persistence of all of the viruses was highest in lake sediment followed by in the feces and the shortest in duck meat at all temperatures. For the AIV subtypes, T-90 values in sediment ranged from 5-11, 13-18, 43-54, and 66-394 days at 30, 20, 10, and 0 ?C, respectively, which were 2-5 times higher than the T-90 values of the viruses in the feces and meat. Although the individual viruses vary in their tenacity, the survival time of influenza viruses was comparatively shorter than that of NDV and ECBO in all substrates. The results of this study suggest that lake sediment may act as a long-term source of influenza viruses in the aquatic habitat while the viruses may remain infectious for extended periods of time in duck feces and meat at low temperatures, allowing persistence of the viruses in the environment over winter.
PMID:
21622783
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21622783