tetano
Editor, Senior Moderator
J Clin Microbiol. 2016 Nov 30. pii: JCM.02045-16. [Epub ahead of print]
[h=1]Performance Evaluation of Allplex? Respiratory Panel 1, 2, and 3 for the Detection of Respiratory Viruses and Influenza A Virus Subtypes.[/h] Huh HJ[SUP]1[/SUP], Kim JY[SUP]2[/SUP], Kwon HJ[SUP]2[/SUP], Yun SA[SUP]2[/SUP], Lee MK[SUP]1[/SUP], Lee NY[SUP]1[/SUP], Kim JW[SUP]1[/SUP], Ki CS[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The Allplex? Respiratory Panel 1, 2, and 3 (Allplex) is a one-step real-time reverse transcription PCR assay for the detection of respiratory viruses (RVs) and influenza A subtypes based on MuDT? technology. Performance of the Allplex assay was compared to the AdvanSure? RV real-time PCR Kit (AdvanSure) and PowerChek? Pandemic H1N1/H3N2/H5N1 Real-time PCR Kit (PowerChek) using 417 clinical respiratory specimens. Compared to the AdvanSure assay for RV detection by each virus, the ranges of positive percent agreement, negative percent agreement, and kappa value were 82.8-100%, 95.5-100%, and 0.85-1.00, respectively. For influenza A virus (INF A) subtyping, the kappa values between the Allplex and PowerChek assays were 0.67 and 1.00 for the INF A H1N1-pdm09 and H3 subtypes, respectively. Uniplex PCR and sequencing for samples with discrepant results demonstrated that the majority of results were concordant with those from the Allplex assay. When testing 24 samples, the turnaround and hands-on time required to perform the Allplex assay were 4 h 15 min and 15 min respectively. In conclusion, the Allplex assay produced results comparable to the AdvanSure and PowerChek assays.
Copyright ? 2016, American Society for Microbiology. All Rights Reserved.
PMID: 27903601 DOI: 10.1128/JCM.02045-16
[PubMed - as supplied by publisher]
[h=1]Performance Evaluation of Allplex? Respiratory Panel 1, 2, and 3 for the Detection of Respiratory Viruses and Influenza A Virus Subtypes.[/h] Huh HJ[SUP]1[/SUP], Kim JY[SUP]2[/SUP], Kwon HJ[SUP]2[/SUP], Yun SA[SUP]2[/SUP], Lee MK[SUP]1[/SUP], Lee NY[SUP]1[/SUP], Kim JW[SUP]1[/SUP], Ki CS[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The Allplex? Respiratory Panel 1, 2, and 3 (Allplex) is a one-step real-time reverse transcription PCR assay for the detection of respiratory viruses (RVs) and influenza A subtypes based on MuDT? technology. Performance of the Allplex assay was compared to the AdvanSure? RV real-time PCR Kit (AdvanSure) and PowerChek? Pandemic H1N1/H3N2/H5N1 Real-time PCR Kit (PowerChek) using 417 clinical respiratory specimens. Compared to the AdvanSure assay for RV detection by each virus, the ranges of positive percent agreement, negative percent agreement, and kappa value were 82.8-100%, 95.5-100%, and 0.85-1.00, respectively. For influenza A virus (INF A) subtyping, the kappa values between the Allplex and PowerChek assays were 0.67 and 1.00 for the INF A H1N1-pdm09 and H3 subtypes, respectively. Uniplex PCR and sequencing for samples with discrepant results demonstrated that the majority of results were concordant with those from the Allplex assay. When testing 24 samples, the turnaround and hands-on time required to perform the Allplex assay were 4 h 15 min and 15 min respectively. In conclusion, the Allplex assay produced results comparable to the AdvanSure and PowerChek assays.
Copyright ? 2016, American Society for Microbiology. All Rights Reserved.
PMID: 27903601 DOI: 10.1128/JCM.02045-16
[PubMed - as supplied by publisher]