tetano
Editor, Senior Moderator
Pathogenic mechanisms of influenza A(H1N1)pdm09 infection elucidated by gene expression profiling
Nobuko Yamashita1,*,
Hirokazu Tsukahara1,
Mitsuru Tsuge1,
Yoshiharu Nagaoka1,
Masato Yashiro1,
Yukie Saito1,
Yosuke Fujii1,
Oka Takashi2,
Tsuneo Morishima1
DOI: 10.1111/ped.12139
Abstract
Background
The pathogenic mechanisms underlying influenza A(H1N1)pdm09 associated central nervous system (CNS) manifestations and pneumonia remain unclear. This study examined A(H1N1)pdm09 host responses using gene expression profiles of patients? peripheral blood.
Methods
Sixteen A(H1N1)pdm09 infected children in three groups were examined: CNS group, with convulsion and altered consciousness, n=6; Pneu group, with pneumonia, n=5; and Control group, of infected control patients, n=5. The signal ratios of the acute to recovery phases in CNS or Pneu were analyzed versus those to the Control.
Results
CNS (619 transcripts) and Pneu (656 transcripts) showed significantly increased signal ratios compared to Control. Regarding the increased ratios of transcripts shown by multiple probes, contactin-associated protein-like 3 transcripts, oleoyl-ACP hydrolase transcripts, and interleukin 1 type 1 receptor were observed in CNS and Pneu. Increased ratios of prostaglandin-endoperoxide synthase 2 and alpha-synuclein were characteristic of CNS. Alkaline phosphatase and the Fc fragment of IgA receptor were characteristic of Pneu. Regarding enriched gene ontology terms, ?response to lipopolysaccharide?, ?innate immune response?, and ?intrinsic to membrane? were observed commonly in CNS and Pneu. Enriched gene ontology terms related to ?hemoglobin? and ?hemostasis? were, respectively, characteristic of CNS and Pneu.
Conclusion
These symptom-associated transcripts might be some clues to the pathogenesis of the A(H1N1)pdm09 infection.
http://onlinelibrary.wiley.com/doi/...ionid=D43B8ECD271C2B57A378A94A351326B7.d03t04
Nobuko Yamashita1,*,
Hirokazu Tsukahara1,
Mitsuru Tsuge1,
Yoshiharu Nagaoka1,
Masato Yashiro1,
Yukie Saito1,
Yosuke Fujii1,
Oka Takashi2,
Tsuneo Morishima1
DOI: 10.1111/ped.12139
Abstract
Background
The pathogenic mechanisms underlying influenza A(H1N1)pdm09 associated central nervous system (CNS) manifestations and pneumonia remain unclear. This study examined A(H1N1)pdm09 host responses using gene expression profiles of patients? peripheral blood.
Methods
Sixteen A(H1N1)pdm09 infected children in three groups were examined: CNS group, with convulsion and altered consciousness, n=6; Pneu group, with pneumonia, n=5; and Control group, of infected control patients, n=5. The signal ratios of the acute to recovery phases in CNS or Pneu were analyzed versus those to the Control.
Results
CNS (619 transcripts) and Pneu (656 transcripts) showed significantly increased signal ratios compared to Control. Regarding the increased ratios of transcripts shown by multiple probes, contactin-associated protein-like 3 transcripts, oleoyl-ACP hydrolase transcripts, and interleukin 1 type 1 receptor were observed in CNS and Pneu. Increased ratios of prostaglandin-endoperoxide synthase 2 and alpha-synuclein were characteristic of CNS. Alkaline phosphatase and the Fc fragment of IgA receptor were characteristic of Pneu. Regarding enriched gene ontology terms, ?response to lipopolysaccharide?, ?innate immune response?, and ?intrinsic to membrane? were observed commonly in CNS and Pneu. Enriched gene ontology terms related to ?hemoglobin? and ?hemostasis? were, respectively, characteristic of CNS and Pneu.
Conclusion
These symptom-associated transcripts might be some clues to the pathogenesis of the A(H1N1)pdm09 infection.
http://onlinelibrary.wiley.com/doi/...ionid=D43B8ECD271C2B57A378A94A351326B7.d03t04