tetano
Editor, Senior Moderator
Emerg Infect Dis. 2014 May;20(5):847-9. doi: 10.3201/eid2005.131364.
PCR for Detection of Oseltamivir Resistance Mutation in Influenza A(H7N9) Virus.
Wang W, Song Z, Guan W, Liu Y, Zhang X, Xu L, Li J, Yuan Z, Hu Y.
Abstract
Sensitive molecular techniques are needed for rapid detection of the R292K oseltamivir-resistant mutant of influenza A(H7/N9) virus strain to monitor its transmission and guide antiviral treatment. We developed a real-time reverse transcription PCR and single nucleotide polymorphism probes to differentiate this mutant strain in mixed virus populations in human specimens.
KEYWORDS:
H7N9, NA 292K mutant virus, R292K wild-type virus, SNP, influenza, influenza A(H7N9) virus, influenza virus, mutation, neuraminidase, oseltamivir resistance, real-time RT-PCR, real-time reverse transcription PCR, single-nucleotide polymorphism, viruses
PMID:
24751348
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/24751348
PCR for Detection of Oseltamivir Resistance Mutation in Influenza A(H7N9) Virus.
Wang W, Song Z, Guan W, Liu Y, Zhang X, Xu L, Li J, Yuan Z, Hu Y.
Abstract
Sensitive molecular techniques are needed for rapid detection of the R292K oseltamivir-resistant mutant of influenza A(H7/N9) virus strain to monitor its transmission and guide antiviral treatment. We developed a real-time reverse transcription PCR and single nucleotide polymorphism probes to differentiate this mutant strain in mixed virus populations in human specimens.
KEYWORDS:
H7N9, NA 292K mutant virus, R292K wild-type virus, SNP, influenza, influenza A(H7N9) virus, influenza virus, mutation, neuraminidase, oseltamivir resistance, real-time RT-PCR, real-time reverse transcription PCR, single-nucleotide polymorphism, viruses
PMID:
24751348
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/24751348