tetano
Editor, Senior Moderator
Eur J Immunol. 2015 Oct 8. doi: 10.1002/eji.201444970. [Epub ahead of print]
[h=1]Pattern recognition receptor mediated downregulation of microRNA-650 fine-tunes MxA expression in dendritic cells infected with Influenza A virus.[/h] Pichulik T[SUP]1,[/SUP][SUP]2[/SUP], Khatamzas E[SUP]1[/SUP], Liu X[SUP]2[/SUP], Brain O[SUP]1,[/SUP][SUP]3[/SUP], Garcia MD[SUP]2[/SUP], Leslie A[SUP]1[/SUP], Danis B[SUP]1[/SUP], Mayer A[SUP]1[/SUP], Baban D[SUP]4[/SUP], Ragoussis J[SUP]4[/SUP], Weber AN[SUP]2[/SUP], Simmons A[SUP]1,[/SUP][SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] MicroRNAs (miRNAs) are important post-transcriptional regulators of gene expression, which have been shown to fine-tune innate immune responses downstream of pattern recognition receptor (PRR) signaling. This study identifies miR-650 as a novel PRR-responsive miRNA which is downregulated upon stimulation of primary human monocyte-derived dendritic cells (MDDCs) with a variety of different microbe-associated molecular patterns (MAMPs). A comprehensive target search combining in silico analysis, transcriptional profiling and reporter assays reveals that miR-650 regulates several well-known interferon-stimulated genes (ISGs), including IFIT2 and MXA. In particular, downregulation of miR-650 in Influenza A-infected MDDCs enhances the expression of MxA and may therefore contribute to the establishment of an antiviral state. Together these findings reveal a novel link between miR-650 and the innate immune response in human MDDCs. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
PMID: 26460926 [PubMed - as supplied by publisher]
[h=1]Pattern recognition receptor mediated downregulation of microRNA-650 fine-tunes MxA expression in dendritic cells infected with Influenza A virus.[/h] Pichulik T[SUP]1,[/SUP][SUP]2[/SUP], Khatamzas E[SUP]1[/SUP], Liu X[SUP]2[/SUP], Brain O[SUP]1,[/SUP][SUP]3[/SUP], Garcia MD[SUP]2[/SUP], Leslie A[SUP]1[/SUP], Danis B[SUP]1[/SUP], Mayer A[SUP]1[/SUP], Baban D[SUP]4[/SUP], Ragoussis J[SUP]4[/SUP], Weber AN[SUP]2[/SUP], Simmons A[SUP]1,[/SUP][SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] MicroRNAs (miRNAs) are important post-transcriptional regulators of gene expression, which have been shown to fine-tune innate immune responses downstream of pattern recognition receptor (PRR) signaling. This study identifies miR-650 as a novel PRR-responsive miRNA which is downregulated upon stimulation of primary human monocyte-derived dendritic cells (MDDCs) with a variety of different microbe-associated molecular patterns (MAMPs). A comprehensive target search combining in silico analysis, transcriptional profiling and reporter assays reveals that miR-650 regulates several well-known interferon-stimulated genes (ISGs), including IFIT2 and MXA. In particular, downregulation of miR-650 in Influenza A-infected MDDCs enhances the expression of MxA and may therefore contribute to the establishment of an antiviral state. Together these findings reveal a novel link between miR-650 and the innate immune response in human MDDCs. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
PMID: 26460926 [PubMed - as supplied by publisher]