tetano
Editor, Senior Moderator
Pathogens
. 2020 Nov 7;9(11):E925.
doi: 10.3390/pathogens9110925.
RNA Secondary Structure as a First Step for Rational Design of the Oligonucleotides towards Inhibition of Influenza A Virus Replication
Marta Szabat[SUP] 1 [/SUP], Dagny Lorent[SUP] 1 [/SUP], Tomasz Czapik[SUP] 1 [/SUP], Maria Tomaszewska[SUP] 1 [/SUP], Elzbieta Kierzek[SUP] 1 [/SUP], Ryszard Kierzek[SUP] 1 [/SUP]
Affiliations
Abstract
Influenza is an important research subject around the world because of its threat to humanity. Influenza A virus (IAV) causes seasonal epidemics and sporadic, but dangerous pandemics. A rapid antigen changes and recombination of the viral RNA genome contribute to the reduced effectiveness of vaccination and anti-influenza drugs. Hence, there is a necessity to develop new antiviral drugs and strategies to limit the influenza spread. IAV is a single-stranded negative sense RNA virus with a genome (viral RNA-vRNA) consisting of eight segments. Segments within influenza virion are assembled into viral ribonucleoprotein (vRNP) complexes that are independent transcription-replication units. Each step in the influenza life cycle is regulated by the RNA and is dependent on its interplay and dynamics. Therefore, viral RNA can be a proper target to design novel therapeutics. Here, we briefly described examples of anti-influenza strategies based on the antisense oligonucleotide (ASO), small interfering RNA (siRNA), microRNA (miRNA) and catalytic nucleic acids. In particular we focused on the vRNA structure-function relationship as well as presented the advantages of using secondary structure information in predicting therapeutic targets and the potential future of this field.
Keywords: RNA interference; RNA structure; antisense oligonucleotides; antiviral strategies; catalytic nucleic acids; influenza A virus; replication.
. 2020 Nov 7;9(11):E925.
doi: 10.3390/pathogens9110925.
RNA Secondary Structure as a First Step for Rational Design of the Oligonucleotides towards Inhibition of Influenza A Virus Replication
Marta Szabat[SUP] 1 [/SUP], Dagny Lorent[SUP] 1 [/SUP], Tomasz Czapik[SUP] 1 [/SUP], Maria Tomaszewska[SUP] 1 [/SUP], Elzbieta Kierzek[SUP] 1 [/SUP], Ryszard Kierzek[SUP] 1 [/SUP]
Affiliations
- PMID: 33171815
- DOI: 10.3390/pathogens9110925
Abstract
Influenza is an important research subject around the world because of its threat to humanity. Influenza A virus (IAV) causes seasonal epidemics and sporadic, but dangerous pandemics. A rapid antigen changes and recombination of the viral RNA genome contribute to the reduced effectiveness of vaccination and anti-influenza drugs. Hence, there is a necessity to develop new antiviral drugs and strategies to limit the influenza spread. IAV is a single-stranded negative sense RNA virus with a genome (viral RNA-vRNA) consisting of eight segments. Segments within influenza virion are assembled into viral ribonucleoprotein (vRNP) complexes that are independent transcription-replication units. Each step in the influenza life cycle is regulated by the RNA and is dependent on its interplay and dynamics. Therefore, viral RNA can be a proper target to design novel therapeutics. Here, we briefly described examples of anti-influenza strategies based on the antisense oligonucleotide (ASO), small interfering RNA (siRNA), microRNA (miRNA) and catalytic nucleic acids. In particular we focused on the vRNA structure-function relationship as well as presented the advantages of using secondary structure information in predicting therapeutic targets and the potential future of this field.
Keywords: RNA interference; RNA structure; antisense oligonucleotides; antiviral strategies; catalytic nucleic acids; influenza A virus; replication.