tetano
Editor, Senior Moderator
Pathogens
. 2021 Nov 22;10(11):1527.
doi: 10.3390/pathogens10111527.
Improving Current Knowledge on Seroprevalence and Genetic Characterization of Swine Influenza Virus in Croatian Pig Farms: A Retrospective Study
Andreja Jungić[SUP] 1 [/SUP], Vladimir Savić[SUP] 2 [/SUP], Josip Madić[SUP] 3 [/SUP], Ljubo Barbić[SUP] 3 [/SUP], Besi Roić[SUP] 1 [/SUP], Dragan Brnić[SUP] 1 [/SUP], Jelena Prpić[SUP] 1 [/SUP], Lorena Jemeršić[SUP] 1 [/SUP], Dinko Novosel[SUP] 4 [/SUP]
Affiliations
Abstract
In a total of 1536 blood serum samples analysed by ELISA, antibodies for IAV nucleoprotein (NP) were detected in 30.3%. Results from HI show that the most common subtype of swIAV in the Croatian pig population was H1N1 (44.6%), followed by H3N2 (42.7%) and H1N2 (26.3%). Antibodies to at least one subtype were detected in 62.19% of blood serum samples. Detection of swIAV antigen was performed by IHC and detected in 8 of 28 lung samples collected post-mortem. The matrix (M) gene was detected in nine of one hundred and forty-two lung tissue samples and in seven of twenty-nine nasopharyngeal swabs. Phylogenetic analysis of amplified HA and NA gene fragments in Croatian isolates suggests the presence of swIAV H1avN1av.
Keywords: Croatia; H1N1; pigs; seroprevalence; swine influenza virus.
. 2021 Nov 22;10(11):1527.
doi: 10.3390/pathogens10111527.
Improving Current Knowledge on Seroprevalence and Genetic Characterization of Swine Influenza Virus in Croatian Pig Farms: A Retrospective Study
Andreja Jungić[SUP] 1 [/SUP], Vladimir Savić[SUP] 2 [/SUP], Josip Madić[SUP] 3 [/SUP], Ljubo Barbić[SUP] 3 [/SUP], Besi Roić[SUP] 1 [/SUP], Dragan Brnić[SUP] 1 [/SUP], Jelena Prpić[SUP] 1 [/SUP], Lorena Jemeršić[SUP] 1 [/SUP], Dinko Novosel[SUP] 4 [/SUP]
Affiliations
- PMID: 34832682
- PMCID: PMC8623915
- DOI: 10.3390/pathogens10111527
Abstract
In a total of 1536 blood serum samples analysed by ELISA, antibodies for IAV nucleoprotein (NP) were detected in 30.3%. Results from HI show that the most common subtype of swIAV in the Croatian pig population was H1N1 (44.6%), followed by H3N2 (42.7%) and H1N2 (26.3%). Antibodies to at least one subtype were detected in 62.19% of blood serum samples. Detection of swIAV antigen was performed by IHC and detected in 8 of 28 lung samples collected post-mortem. The matrix (M) gene was detected in nine of one hundred and forty-two lung tissue samples and in seven of twenty-nine nasopharyngeal swabs. Phylogenetic analysis of amplified HA and NA gene fragments in Croatian isolates suggests the presence of swIAV H1avN1av.
Keywords: Croatia; H1N1; pigs; seroprevalence; swine influenza virus.