tetano
Editor, Senior Moderator
Pathogens
. 2022 Nov 18;11(11):1378.
doi: 10.3390/pathogens11111378.
Diagnosis of SARS-CoV-2 during the Pandemic by Multiplex RT-rPCR hCoV Test: Future Perspectives
Alessio Danilo Inchingolo[SUP] 1 [/SUP], Ciro Isacco Gargiulo[SUP] 1 [/SUP], Giuseppina Malcangi[SUP] 1 [/SUP], Anna Maria Ciocia[SUP] 1 [/SUP], Assunta Patano[SUP] 1 [/SUP], Daniela Azzollini[SUP] 1 [/SUP], Fabio Piras[SUP] 1 [/SUP], Giuseppe Barile[SUP] 1 [/SUP], Vito Settanni[SUP] 1 [/SUP], Antonio Mancini[SUP] 1 [/SUP], Grazia Garofoli[SUP] 1 [/SUP], Giulia Palmieri[SUP] 1 [/SUP], Chiara Di Pede[SUP] 1 [/SUP], Biagio Rapone[SUP] 1 [/SUP], Angelo Michele Inchingolo[SUP] 1 [/SUP], Megan Jones[SUP] 1 [/SUP], Alberto Corriero[SUP] 2 [/SUP], Nicola Brienza[SUP] 2 [/SUP], Antonio Parisi[SUP] 3 [/SUP], Angelica Bianco[SUP] 3 [/SUP], Loredana Capozzi[SUP] 3 [/SUP], Laura Del Sambro[SUP] 3 [/SUP], Domenico Simone[SUP] 3 [/SUP], Ioana Roxana Bordea[SUP] 4 [/SUP], Gianluca Martino Tartaglia[SUP] 5 [/SUP], Antonio Scarano[SUP] 6 [/SUP], Felice Lorusso[SUP] 6 [/SUP], Luigi Macchia[SUP] 7 [/SUP], Giovanni Migliore[SUP] 8 [/SUP], Van Hung Pham[SUP] 9 10 [/SUP], Gianna Dipalma[SUP] 1 [/SUP], Francesco Inchingolo[SUP] 1 [/SUP]
Affiliations
Abstract
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has rapidly become a significant threat to public health. However, among the Coronaviridae family members, there are other viruses that can also cause infections in humans. Among these, severe acute respiratory syndrome (SARS-CoV) and Middle East respiratory syndrome (MERS-CoV) have posed significant threats to human health in the past. Other human pathogenic coronaviruses have been identified, and they are known to cause respiratory diseases with manifestations ranging from mild to severe. In this study, we evaluated the performance of a multiplex RT-rPCR specific to seven human pathogenic coronaviruses in mainly detecting SARS-CoV-2 directly from nasopharyngeal swabs obtained from suspected COVID-19 infected patients, while simultaneously detecting different human pathogenic coronaviruses in case these were also present. We tested 1195 clinical samples suspected of COVID-19 infection. The assay identified that 69% of the samples tested positive for SARS-CoV-2 (1195), which was confirmed using another SARS-CoV-2 RT-PCR kit available in our laboratory. None of these clinical samples were positive for SARS-CoV, MERS-CoV or HCoV. This means that during the endemic phase of COVID-19, infection with other human pathogenic coronaviruses, even the common cold coronavirus (HCoV), is very uncommon. Our study also confirmed that the multiplex RT-rPCR is a sensitive assay for detecting SARS-CoV-2 regardless of differences among the variants. This multiplex RT-rPCR is also time- and cost-saving and very easy to apply in the diagnostic laboratory due to its simple procedure and its stability in storage after preparation. These features make the assay a valuable approach in screening procedures for the rapid detection of SARS-CoV-2 and other human pathogenic coronaviruses that could affect public health.
Keywords: COVID-19; SARS-CoV; SARS-CoV-2; antispike; diagnostic accuracy; human pathogenic coronavirus; multiplex RT-rPCR; vaccines.
. 2022 Nov 18;11(11):1378.
doi: 10.3390/pathogens11111378.
Diagnosis of SARS-CoV-2 during the Pandemic by Multiplex RT-rPCR hCoV Test: Future Perspectives
Alessio Danilo Inchingolo[SUP] 1 [/SUP], Ciro Isacco Gargiulo[SUP] 1 [/SUP], Giuseppina Malcangi[SUP] 1 [/SUP], Anna Maria Ciocia[SUP] 1 [/SUP], Assunta Patano[SUP] 1 [/SUP], Daniela Azzollini[SUP] 1 [/SUP], Fabio Piras[SUP] 1 [/SUP], Giuseppe Barile[SUP] 1 [/SUP], Vito Settanni[SUP] 1 [/SUP], Antonio Mancini[SUP] 1 [/SUP], Grazia Garofoli[SUP] 1 [/SUP], Giulia Palmieri[SUP] 1 [/SUP], Chiara Di Pede[SUP] 1 [/SUP], Biagio Rapone[SUP] 1 [/SUP], Angelo Michele Inchingolo[SUP] 1 [/SUP], Megan Jones[SUP] 1 [/SUP], Alberto Corriero[SUP] 2 [/SUP], Nicola Brienza[SUP] 2 [/SUP], Antonio Parisi[SUP] 3 [/SUP], Angelica Bianco[SUP] 3 [/SUP], Loredana Capozzi[SUP] 3 [/SUP], Laura Del Sambro[SUP] 3 [/SUP], Domenico Simone[SUP] 3 [/SUP], Ioana Roxana Bordea[SUP] 4 [/SUP], Gianluca Martino Tartaglia[SUP] 5 [/SUP], Antonio Scarano[SUP] 6 [/SUP], Felice Lorusso[SUP] 6 [/SUP], Luigi Macchia[SUP] 7 [/SUP], Giovanni Migliore[SUP] 8 [/SUP], Van Hung Pham[SUP] 9 10 [/SUP], Gianna Dipalma[SUP] 1 [/SUP], Francesco Inchingolo[SUP] 1 [/SUP]
Affiliations
- PMID: 36422629
- DOI: 10.3390/pathogens11111378
Abstract
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has rapidly become a significant threat to public health. However, among the Coronaviridae family members, there are other viruses that can also cause infections in humans. Among these, severe acute respiratory syndrome (SARS-CoV) and Middle East respiratory syndrome (MERS-CoV) have posed significant threats to human health in the past. Other human pathogenic coronaviruses have been identified, and they are known to cause respiratory diseases with manifestations ranging from mild to severe. In this study, we evaluated the performance of a multiplex RT-rPCR specific to seven human pathogenic coronaviruses in mainly detecting SARS-CoV-2 directly from nasopharyngeal swabs obtained from suspected COVID-19 infected patients, while simultaneously detecting different human pathogenic coronaviruses in case these were also present. We tested 1195 clinical samples suspected of COVID-19 infection. The assay identified that 69% of the samples tested positive for SARS-CoV-2 (1195), which was confirmed using another SARS-CoV-2 RT-PCR kit available in our laboratory. None of these clinical samples were positive for SARS-CoV, MERS-CoV or HCoV. This means that during the endemic phase of COVID-19, infection with other human pathogenic coronaviruses, even the common cold coronavirus (HCoV), is very uncommon. Our study also confirmed that the multiplex RT-rPCR is a sensitive assay for detecting SARS-CoV-2 regardless of differences among the variants. This multiplex RT-rPCR is also time- and cost-saving and very easy to apply in the diagnostic laboratory due to its simple procedure and its stability in storage after preparation. These features make the assay a valuable approach in screening procedures for the rapid detection of SARS-CoV-2 and other human pathogenic coronaviruses that could affect public health.
Keywords: COVID-19; SARS-CoV; SARS-CoV-2; antispike; diagnostic accuracy; human pathogenic coronavirus; multiplex RT-rPCR; vaccines.