tetano
Editor, Senior Moderator
Pathogens
. 2021 May 22;10(6):637.
doi: 10.3390/pathogens10060637.
Detection of Anti-Nucleocapsid Antibody in COVID-19 Patients in Bangladesh Is not Correlated with Previous Dengue Infection
Simon D Lytton[SUP] 1 [/SUP], Mahmuda Yeasmin[SUP] 2 [/SUP], Asish Kumar Ghosh[SUP] 3 [/SUP], Md Rakibul Hassan Bulbul[SUP] 4 [/SUP], Md Maruf Ahmed Molla[SUP] 2 [/SUP], Martha Herr[SUP] 5 [/SUP], Helmut Duchmann[SUP] 5 [/SUP], Md Mohiuddin Sharif[SUP] 3 [/SUP], Tasnim Nafisa[SUP] 2 [/SUP], Md Robed Amin[SUP] 3 [/SUP], Nur Hosen[SUP] 2 [/SUP], Md Tanvir Rahman[SUP] 6 [/SUP], Sumaiya Islam[SUP] 7 [/SUP], Alimul Islam[SUP] 6 [/SUP], Abul Khair Mohammad Shamsuzzaman[SUP] 2 [/SUP]
Affiliations
Abstract
Background: The assessment of antibody responses to severe acute respiratory syndrome coronavirus-2 is potentially confounded by exposures to flaviviruses. The aims of the present research were to determine whether anti-dengue antibodies affect the viral load and the detection of anti-coronavirus nucleocapsid (N)-protein antibodies in coronavirus infectious disease 2019 (COVID-19) in Bangladesh.
Methods: Viral RNA was evaluated in swab specimens from 115 COVID-19 patients by real-time reverse transcription polymerase chain reaction (rT-PCR). The anti-N-protein antibodies, anti-dengue virus E-protein antibodies and the dengue non-structural protein-1 were determined in serum from 115 COVID-19 patients, 30 acute dengue fever pre-COVID-19 pandemic and nine normal controls by ELISA.
Results: The concentrations of viral RNA in the nasopharyngeal; Ct median (95% CI); 22 (21.9-23.3) was significantly higher than viral RNA concentrations in oropharyngeal swabs; and 29 (27-30.5) p < 0.0001. Viral RNA concentrations were not correlated with-dengue IgG levels. The anti-nucleocapsid antibodies were IgA 27% positive and IgG 35% positive at days 1 to 8 post-onset of COVID-19 symptoms versus IgA 0% and IgG 0% in dengue patients, p < 0.0001. The levels of anti- nucleocapsid IgA or IgG versus the levels of anti-dengue IgM or IgG revealed no significant correlations.
Conclusions: Viral RNA and anti-nucleocapsid antibodies were detected in COVID-19 patients from dengue-endemic regions of Bangladesh, independently of the dengue IgG levels.
Keywords: COVID-19; DENV; ELISA; IgA; IgG; IgM; N-protein; SARS-CoV-2; dengue fever; rT-PCR.
. 2021 May 22;10(6):637.
doi: 10.3390/pathogens10060637.
Detection of Anti-Nucleocapsid Antibody in COVID-19 Patients in Bangladesh Is not Correlated with Previous Dengue Infection
Simon D Lytton[SUP] 1 [/SUP], Mahmuda Yeasmin[SUP] 2 [/SUP], Asish Kumar Ghosh[SUP] 3 [/SUP], Md Rakibul Hassan Bulbul[SUP] 4 [/SUP], Md Maruf Ahmed Molla[SUP] 2 [/SUP], Martha Herr[SUP] 5 [/SUP], Helmut Duchmann[SUP] 5 [/SUP], Md Mohiuddin Sharif[SUP] 3 [/SUP], Tasnim Nafisa[SUP] 2 [/SUP], Md Robed Amin[SUP] 3 [/SUP], Nur Hosen[SUP] 2 [/SUP], Md Tanvir Rahman[SUP] 6 [/SUP], Sumaiya Islam[SUP] 7 [/SUP], Alimul Islam[SUP] 6 [/SUP], Abul Khair Mohammad Shamsuzzaman[SUP] 2 [/SUP]
Affiliations
- PMID: 34067281
- DOI: 10.3390/pathogens10060637
Abstract
Background: The assessment of antibody responses to severe acute respiratory syndrome coronavirus-2 is potentially confounded by exposures to flaviviruses. The aims of the present research were to determine whether anti-dengue antibodies affect the viral load and the detection of anti-coronavirus nucleocapsid (N)-protein antibodies in coronavirus infectious disease 2019 (COVID-19) in Bangladesh.
Methods: Viral RNA was evaluated in swab specimens from 115 COVID-19 patients by real-time reverse transcription polymerase chain reaction (rT-PCR). The anti-N-protein antibodies, anti-dengue virus E-protein antibodies and the dengue non-structural protein-1 were determined in serum from 115 COVID-19 patients, 30 acute dengue fever pre-COVID-19 pandemic and nine normal controls by ELISA.
Results: The concentrations of viral RNA in the nasopharyngeal; Ct median (95% CI); 22 (21.9-23.3) was significantly higher than viral RNA concentrations in oropharyngeal swabs; and 29 (27-30.5) p < 0.0001. Viral RNA concentrations were not correlated with-dengue IgG levels. The anti-nucleocapsid antibodies were IgA 27% positive and IgG 35% positive at days 1 to 8 post-onset of COVID-19 symptoms versus IgA 0% and IgG 0% in dengue patients, p < 0.0001. The levels of anti- nucleocapsid IgA or IgG versus the levels of anti-dengue IgM or IgG revealed no significant correlations.
Conclusions: Viral RNA and anti-nucleocapsid antibodies were detected in COVID-19 patients from dengue-endemic regions of Bangladesh, independently of the dengue IgG levels.
Keywords: COVID-19; DENV; ELISA; IgA; IgG; IgM; N-protein; SARS-CoV-2; dengue fever; rT-PCR.