tetano
Editor, Senior Moderator
J Virol. 2016 Jun 8. pii: JVI.00772-16. [Epub ahead of print]
[h=1]Pandemic-seasonal H1N1 reassortants recovered from patient material display a phenotype similar to the seasonal parent.[/h] Sonnberg S[SUP]1[/SUP], Ducatez MF[SUP]2[/SUP], DeBeauchamp J[SUP]2[/SUP], Crumpton JC[SUP]2[/SUP], Rubrum A[SUP]2[/SUP], Sharp B[SUP]2[/SUP], Hall RJ[SUP]3[/SUP], Peacey M[SUP]3[/SUP], Huang S[SUP]3[/SUP], Webby RJ[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] We have previously shown that eleven patients became naturally co-infected with seasonal H1N1 (A/H1N1) and pandemic H1N1 (pdm/H1N1) during the Southern hemisphere winter of 2009 in New Zealand. Reassortment of influenza A viruses is readily observed during co-infection of host animals and in vitro, however, reports of reassortment occurring naturally in humans are rare. Using clinical specimen material, we show reassortment between the two co-infecting viruses occurred with high likelihood directly in one of the previously identified patients. Despite the lack of spread of these reassortants in the community, we did not find them to be attenuated in several model systems for viral replication and virus transmission: multistep growth curves in differentiated human bronchial epithelial cells revealed no growth deficiency in six recovered reassortants when compared to A/H1N1 and pdm/H1N1 isolates. Two reassortant viruses were assessed in ferrets and showed transmission to aerosol contacts. This study demonstrates that influenza virus reassortants can arise in naturally co-infected patients.
[h=4]IMPORTANCE:[/h] Reassortment of influenza A viruses is an important driver of virus evolution, but little has been done to address humans as hosts for the generation of novel influenza viruses. We show here that multiple reassortant viruses were generated during natural co-infection of a patient with pandemic H1N1 (2009) and seasonal H1N1 influenza A viruses. Though apparently fit in model systems, these reassortants did not become established in the wider population, presumably due to herd immunity against their seasonal H1 antigen.
Copyright ? 2016, American Society for Microbiology. All Rights Reserved.
PMID: 27279619 [PubMed - as supplied by publisher]
[h=1]Pandemic-seasonal H1N1 reassortants recovered from patient material display a phenotype similar to the seasonal parent.[/h] Sonnberg S[SUP]1[/SUP], Ducatez MF[SUP]2[/SUP], DeBeauchamp J[SUP]2[/SUP], Crumpton JC[SUP]2[/SUP], Rubrum A[SUP]2[/SUP], Sharp B[SUP]2[/SUP], Hall RJ[SUP]3[/SUP], Peacey M[SUP]3[/SUP], Huang S[SUP]3[/SUP], Webby RJ[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] We have previously shown that eleven patients became naturally co-infected with seasonal H1N1 (A/H1N1) and pandemic H1N1 (pdm/H1N1) during the Southern hemisphere winter of 2009 in New Zealand. Reassortment of influenza A viruses is readily observed during co-infection of host animals and in vitro, however, reports of reassortment occurring naturally in humans are rare. Using clinical specimen material, we show reassortment between the two co-infecting viruses occurred with high likelihood directly in one of the previously identified patients. Despite the lack of spread of these reassortants in the community, we did not find them to be attenuated in several model systems for viral replication and virus transmission: multistep growth curves in differentiated human bronchial epithelial cells revealed no growth deficiency in six recovered reassortants when compared to A/H1N1 and pdm/H1N1 isolates. Two reassortant viruses were assessed in ferrets and showed transmission to aerosol contacts. This study demonstrates that influenza virus reassortants can arise in naturally co-infected patients.
[h=4]IMPORTANCE:[/h] Reassortment of influenza A viruses is an important driver of virus evolution, but little has been done to address humans as hosts for the generation of novel influenza viruses. We show here that multiple reassortant viruses were generated during natural co-infection of a patient with pandemic H1N1 (2009) and seasonal H1N1 influenza A viruses. Though apparently fit in model systems, these reassortants did not become established in the wider population, presumably due to herd immunity against their seasonal H1 antigen.
Copyright ? 2016, American Society for Microbiology. All Rights Reserved.
PMID: 27279619 [PubMed - as supplied by publisher]