tetano
Editor, Senior Moderator
NPJ Vaccines. 2019 Dec 6;4:51. doi: 10.1038/s41541-019-0147-z. eCollection 2019. [h=1]Pandemic influenza virus vaccines boost hemagglutinin stalk-specific antibody responses in primed adult and pediatric cohorts.[/h]
Nachbagauer R[SUP]1[/SUP], Salaun B[SUP]2[/SUP], Stadlbauer D[SUP]1[/SUP], Behzadi MA[SUP]1[/SUP], Friel D[SUP]3[/SUP], Rajabhathor A[SUP]1[/SUP], Choi A[SUP]1,[/SUP][SUP]4[/SUP], Albrecht RA[SUP]1,[/SUP][SUP]5[/SUP], Debois M[SUP]3[/SUP], Garc?a-Sastre A[SUP]1,[/SUP][SUP]5,[/SUP][SUP]6[/SUP], Rouxel RN[SUP]2[/SUP], Sun W[SUP]1[/SUP], Palese P[SUP]1,[/SUP][SUP]6[/SUP], Mallett CP[SUP]7[/SUP], Innis BL[SUP]8,[/SUP][SUP]9[/SUP], Krammer F[SUP]#[/SUP][SUP]1[/SUP], Claeys C[SUP]#[/SUP][SUP]10,[/SUP][SUP]11[/SUP].
[h=3]Author information[/h] 1 1Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY USA. 2 2GSK, Rixensart, Belgium. 3 3GSK, Wavre, Belgium. 4 4Graduate School of Biomedical Services, Icahn School of Medicine at Mount Sinai, New York, NY USA. 5 5Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, NY USA. 6 6Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY USA. 7 7GSK, Rockville, MD 20850 USA. 8 8GSK, King of Prussia, PA USA. 9 10Present Address: PATH, Washington, DC, USA. 10 9GSK, Rixensart, Belgium. 11 Present Address: Spmt-Arista Asbl, Brussel, Belgium. # Contributed equally
[h=3]Abstract[/h] Licensed influenza virus vaccines target the head domain of the hemagglutinin (HA) glycoprotein which undergoes constant antigenic drift. The highly conserved HA stalk domain is an attractive target to increase immunologic breadth required for universal influenza virus vaccines. We tested the hypothesis that immunization with a pandemic influenza virus vaccine boosts pre-existing anti-stalk antibodies. We used chimeric cH6/1, full length H2 and H18 HA antigens in an ELISA to measure anti-stalk antibodies in recipients participating in clinical trials of A/H1N1, A/H5N1 and A/H9N2 vaccines. The vaccines induced high titers of anti-H1 stalk antibodies in adults and children, with higher titers elicited by AS03-adjuvanted vaccines. We also observed cross-reactivity to H2 and H18 HAs. The A/H9N2 vaccine elicited plasmablast and memory B-cell responses. Post-vaccination serum from vaccinees protected mice against lethal challenge with cH6/1N5 and cH5/3N4 viruses. These findings support the concept of a chimeric HA stalk-based universal influenza virus vaccine. clinicaltrials.gov: NCT02415842.
? The Author(s) 2019.
[h=4]KEYWORDS:[/h] Adjuvants; Antibodies; Immunological memory; Inactivated vaccines; Influenza virus
PMID: 31839997 PMCID: PMC6898674 DOI: 10.1038/s41541-019-0147-z
Nachbagauer R[SUP]1[/SUP], Salaun B[SUP]2[/SUP], Stadlbauer D[SUP]1[/SUP], Behzadi MA[SUP]1[/SUP], Friel D[SUP]3[/SUP], Rajabhathor A[SUP]1[/SUP], Choi A[SUP]1,[/SUP][SUP]4[/SUP], Albrecht RA[SUP]1,[/SUP][SUP]5[/SUP], Debois M[SUP]3[/SUP], Garc?a-Sastre A[SUP]1,[/SUP][SUP]5,[/SUP][SUP]6[/SUP], Rouxel RN[SUP]2[/SUP], Sun W[SUP]1[/SUP], Palese P[SUP]1,[/SUP][SUP]6[/SUP], Mallett CP[SUP]7[/SUP], Innis BL[SUP]8,[/SUP][SUP]9[/SUP], Krammer F[SUP]#[/SUP][SUP]1[/SUP], Claeys C[SUP]#[/SUP][SUP]10,[/SUP][SUP]11[/SUP].
[h=3]Author information[/h] 1 1Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY USA. 2 2GSK, Rixensart, Belgium. 3 3GSK, Wavre, Belgium. 4 4Graduate School of Biomedical Services, Icahn School of Medicine at Mount Sinai, New York, NY USA. 5 5Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, NY USA. 6 6Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY USA. 7 7GSK, Rockville, MD 20850 USA. 8 8GSK, King of Prussia, PA USA. 9 10Present Address: PATH, Washington, DC, USA. 10 9GSK, Rixensart, Belgium. 11 Present Address: Spmt-Arista Asbl, Brussel, Belgium. # Contributed equally
[h=3]Abstract[/h] Licensed influenza virus vaccines target the head domain of the hemagglutinin (HA) glycoprotein which undergoes constant antigenic drift. The highly conserved HA stalk domain is an attractive target to increase immunologic breadth required for universal influenza virus vaccines. We tested the hypothesis that immunization with a pandemic influenza virus vaccine boosts pre-existing anti-stalk antibodies. We used chimeric cH6/1, full length H2 and H18 HA antigens in an ELISA to measure anti-stalk antibodies in recipients participating in clinical trials of A/H1N1, A/H5N1 and A/H9N2 vaccines. The vaccines induced high titers of anti-H1 stalk antibodies in adults and children, with higher titers elicited by AS03-adjuvanted vaccines. We also observed cross-reactivity to H2 and H18 HAs. The A/H9N2 vaccine elicited plasmablast and memory B-cell responses. Post-vaccination serum from vaccinees protected mice against lethal challenge with cH6/1N5 and cH5/3N4 viruses. These findings support the concept of a chimeric HA stalk-based universal influenza virus vaccine. clinicaltrials.gov: NCT02415842.
? The Author(s) 2019.
[h=4]KEYWORDS:[/h] Adjuvants; Antibodies; Immunological memory; Inactivated vaccines; Influenza virus
PMID: 31839997 PMCID: PMC6898674 DOI: 10.1038/s41541-019-0147-z