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Vaccine
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doi:10.1016/j.vaccine.2011.12.083 | How to Cite or Link Using DOI
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Pandemic H1N1 influenza virus-like particles are immunogenic and provide protective immunity to pigs
Hyun-Mi Pyoa, Aleksandar Masicb, Nizihti Woldeabb, Carissa Embury-Hyattc, Li Lina, Yeun-Kyung Shind, Jae-Young Songd, Shawn Babiukb, c, Yan Zhoua, Corresponding Author Contact Information, E-mail The Corresponding Author
a Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada
b Department of Immunology, University of Manitoba, Winnipeg, MB, Canada R3E 0T5
c National Centre for Foreign Animal Disease-Canadian Food Inspection Agency, Winnipeg, MB, R3E 3M4, Canada
d Animal, Plant and Fisheries Quarantine and Inspection Agency, 175 Anyangro, Manangu, Anyang, Gyeonggido, Republic of Korea
Received 28 September 2011; revised 23 November 2011; Accepted 17 December 2011. Available online 27 December 2011.
Abstract
The outbreak of the 2009 influenza pandemic underscored the important role of swine in influenza virus evolution and the emergence of novel viruses with pandemic potential. Vaccination is the most common practice to control swine influenza in swine industry. Influenza virus-like particle (VLP) vaccines are an alternative approach and have been demonstrated to be immunogenic and confer protection against influenza virus challenge in chickens, mice and ferrets. In this study, we generated VLPs consisting of HA, NA and M1 proteins derived from pandemic virus A/California/04/2009 in insect cells. The immunogenicity and efficacy following vaccination of VLPs were evaluated in swine. Our data showed that vaccination using VLPs elicited robust levels of serum IgG, mucosal IgA, and viral neutralizing antibodies against A/Sw/Manitoba/MAFRI32/2009 H1N1. Following challenge with pandemic H1N1 2009, vaccinated pigs were protected, displaying reduced lung lesions, virus shedding and inhibition of virus replication in the lungs compared to non-vaccinated control pigs. Thus, VLPs can serve as a promising vaccination strategy to control influenza in swine.
http://www.sciencedirect.com/science/article/pii/S0264410X11020329
In Press, Uncorrected Proof - Note to users
doi:10.1016/j.vaccine.2011.12.083 | How to Cite or Link Using DOI
Permissions & Reprints
Pandemic H1N1 influenza virus-like particles are immunogenic and provide protective immunity to pigs
Hyun-Mi Pyoa, Aleksandar Masicb, Nizihti Woldeabb, Carissa Embury-Hyattc, Li Lina, Yeun-Kyung Shind, Jae-Young Songd, Shawn Babiukb, c, Yan Zhoua, Corresponding Author Contact Information, E-mail The Corresponding Author
a Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada
b Department of Immunology, University of Manitoba, Winnipeg, MB, Canada R3E 0T5
c National Centre for Foreign Animal Disease-Canadian Food Inspection Agency, Winnipeg, MB, R3E 3M4, Canada
d Animal, Plant and Fisheries Quarantine and Inspection Agency, 175 Anyangro, Manangu, Anyang, Gyeonggido, Republic of Korea
Received 28 September 2011; revised 23 November 2011; Accepted 17 December 2011. Available online 27 December 2011.
Abstract
The outbreak of the 2009 influenza pandemic underscored the important role of swine in influenza virus evolution and the emergence of novel viruses with pandemic potential. Vaccination is the most common practice to control swine influenza in swine industry. Influenza virus-like particle (VLP) vaccines are an alternative approach and have been demonstrated to be immunogenic and confer protection against influenza virus challenge in chickens, mice and ferrets. In this study, we generated VLPs consisting of HA, NA and M1 proteins derived from pandemic virus A/California/04/2009 in insect cells. The immunogenicity and efficacy following vaccination of VLPs were evaluated in swine. Our data showed that vaccination using VLPs elicited robust levels of serum IgG, mucosal IgA, and viral neutralizing antibodies against A/Sw/Manitoba/MAFRI32/2009 H1N1. Following challenge with pandemic H1N1 2009, vaccinated pigs were protected, displaying reduced lung lesions, virus shedding and inhibition of virus replication in the lungs compared to non-vaccinated control pigs. Thus, VLPs can serve as a promising vaccination strategy to control influenza in swine.
http://www.sciencedirect.com/science/article/pii/S0264410X11020329