tetano
Editor, Senior Moderator
Osong Public Health Res Perspect. 2020 Feb;11(1):3-7. doi: 10.24171/j.phrp.2020.11.1.02. [h=1]Identification of Coronavirus Isolated from a Patient in Korea with COVID-19.[/h]
Kim JM[SUP]1[/SUP], Chung YS[SUP]1[/SUP], Jo HJ[SUP]1[/SUP], Lee NJ[SUP]1[/SUP], Kim MS[SUP]1[/SUP], Woo SH[SUP]1[/SUP], Park S[SUP]1[/SUP], Kim JW[SUP]2[/SUP], Kim HM[SUP]1[/SUP], Han MG[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]Objectives:[/h] Following reports of patients with unexplained pneumonia at the end of December 2019 in Wuhan, China, the causative agent was identified as coronavirus (SARS-CoV-2), and the 2019 novel coronavirus disease was named COVID-19 by the World Health Organization. Putative patients with COVID-19 have been identified in South Korea, and attempts have been made to isolate the pathogen from these patients.
[h=4]Methods:[/h] Upper and lower respiratory tract secretion samples from putative patients with COVID-19 were inoculated onto cells to isolate the virus. Full genome sequencing and electron microscopy were used to identify the virus.
[h=4]Results:[/h] The virus replicated in Vero cells and cytopathic effects were observed. Full genome sequencing showed that the virus genome exhibited sequence homology of more than 99.9% with SARS-CoV-2 which was isolated from patients from other countries, for instance China. Sequence homology of SARS-CoV-2 with SARS-CoV, and MERS-CoV was 77.5% and 50%, respectively. Coronavirus-specific morphology was observed by electron microscopy in virus-infected Vero cells.
[h=4]Conclusion:[/h] SARS-CoV-2 was isolated from putative patients with unexplained pneumonia and intermittent coughing and fever. The isolated virus was named BetaCoV/Korea/KCDC03/2020.
Copyright ?2020, Korea Centers for Disease Control and Prevention.
[h=4]KEYWORDS:[/h] COVID-19; SARS-CoV-2; coronavirus; isolation; pneumonia
PMID: 32149036 PMCID: PMC7045880 DOI: 10.24171/j.phrp.2020.11.1.02
Kim JM[SUP]1[/SUP], Chung YS[SUP]1[/SUP], Jo HJ[SUP]1[/SUP], Lee NJ[SUP]1[/SUP], Kim MS[SUP]1[/SUP], Woo SH[SUP]1[/SUP], Park S[SUP]1[/SUP], Kim JW[SUP]2[/SUP], Kim HM[SUP]1[/SUP], Han MG[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]Objectives:[/h] Following reports of patients with unexplained pneumonia at the end of December 2019 in Wuhan, China, the causative agent was identified as coronavirus (SARS-CoV-2), and the 2019 novel coronavirus disease was named COVID-19 by the World Health Organization. Putative patients with COVID-19 have been identified in South Korea, and attempts have been made to isolate the pathogen from these patients.
[h=4]Methods:[/h] Upper and lower respiratory tract secretion samples from putative patients with COVID-19 were inoculated onto cells to isolate the virus. Full genome sequencing and electron microscopy were used to identify the virus.
[h=4]Results:[/h] The virus replicated in Vero cells and cytopathic effects were observed. Full genome sequencing showed that the virus genome exhibited sequence homology of more than 99.9% with SARS-CoV-2 which was isolated from patients from other countries, for instance China. Sequence homology of SARS-CoV-2 with SARS-CoV, and MERS-CoV was 77.5% and 50%, respectively. Coronavirus-specific morphology was observed by electron microscopy in virus-infected Vero cells.
[h=4]Conclusion:[/h] SARS-CoV-2 was isolated from putative patients with unexplained pneumonia and intermittent coughing and fever. The isolated virus was named BetaCoV/Korea/KCDC03/2020.
Copyright ?2020, Korea Centers for Disease Control and Prevention.
[h=4]KEYWORDS:[/h] COVID-19; SARS-CoV-2; coronavirus; isolation; pneumonia
PMID: 32149036 PMCID: PMC7045880 DOI: 10.24171/j.phrp.2020.11.1.02