tetano
Editor, Senior Moderator
Osong Public Health Res Perspect
. 2020 Jun;11(3):112-117.
doi: 10.24171/j.phrp.2020.11.3.02.
Detection and Isolation of SARS-CoV-2 in Serum, Urine, and Stool Specimens of COVID-19 Patients From the Republic of Korea
Jeong-Min Kim[SUP] 1 [/SUP], Heui Man Kim[SUP] 1 [/SUP], Eun Jung Lee[SUP] 1 [/SUP], Hye Jun Jo[SUP] 1 [/SUP], Youngsil Yoon[SUP] 1 [/SUP], Nam-Joo Lee[SUP] 1 [/SUP], Junseock Son[SUP] 1 [/SUP], Ye-Ji Lee[SUP] 1 [/SUP], Mi Seon Kim[SUP] 1 [/SUP], Yong-Pyo Lee[SUP] 1 [/SUP], Su-Jin Chae[SUP] 1 [/SUP], Kye Ryeong Park[SUP] 1 [/SUP], Seung-Rye Cho[SUP] 1 [/SUP], Sehee Park[SUP] 1 [/SUP], Su Jin Kim[SUP] 1 [/SUP], Eunbyeol Wang[SUP] 1 [/SUP], SangHee Woo[SUP] 1 [/SUP], Aram Lim[SUP] 1 [/SUP], Su-Jin Park[SUP] 1 [/SUP], JunHyeong Jang[SUP] 1 [/SUP], Yoon-Seok Chung[SUP] 1 [/SUP], Bum Sik Chin[SUP] 2 [/SUP], Jin-Soo Lee[SUP] 3 [/SUP], Duko Lim[SUP] 4 [/SUP], Myung-Guk Han[SUP] 1 [/SUP], Cheon Kwon Yoo[SUP] 5 [/SUP]
Affiliations
Abstract
Objectives: Coronavirus Disease-19 (COVID-19) is a respiratory infection characterized by the main symptoms of pneumonia and fever. It is caused by the novel coronavirus severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2), which is known to spread via respiratory droplets. We aimed to determine the rate and likelihood of SARS-CoV-2 transmission from COVID-19 patients through non-respiratory routes.
Methods: Serum, urine, and stool samples were collected from 74 hospitalized patients diagnosed with COVID-19 based on the detection of SARS-CoV-2 in respiratory samples. The SARS-CoV-2 RNA genome was extracted from each specimen and real-time reverse transcription polymerase chain reaction performed. CaCo-2 cells were inoculated with the specimens containing the SARS-COV-2 genome, and subcultured for virus isolation. After culturing, viral replication in the cell supernatant was assessed.
Results: Of the samples collected from 74 COVID-19 patients, SARS-CoV-2 was detected in 15 serum, urine, or stool samples. The virus detection rate in the serum, urine, and stool samples were 2.8% (9/323), 0.8% (2/247), and 10.1% (13/129), and the mean viral load was 1,210 ? 1,861, 79 ? 30, and 3,176 ? 7,208 copy/μL, respectively. However, the SARS-CoV-2 was not isolated by the culture method from the samples that tested positive for the SARS-CoV-2 gene.
Conclusion: While the virus remained detectable in the respiratory samples of COVID-19 patients for several days after hospitalization, its detection in the serum, urine, and stool samples was intermittent. Since the virus could not be isolated from the SARS-COV-2-positive samples, the risk of viral transmission via stool and urine is expected to be low.
Keywords: COVID-19; SARS-CoV-2; transmission.
. 2020 Jun;11(3):112-117.
doi: 10.24171/j.phrp.2020.11.3.02.
Detection and Isolation of SARS-CoV-2 in Serum, Urine, and Stool Specimens of COVID-19 Patients From the Republic of Korea
Jeong-Min Kim[SUP] 1 [/SUP], Heui Man Kim[SUP] 1 [/SUP], Eun Jung Lee[SUP] 1 [/SUP], Hye Jun Jo[SUP] 1 [/SUP], Youngsil Yoon[SUP] 1 [/SUP], Nam-Joo Lee[SUP] 1 [/SUP], Junseock Son[SUP] 1 [/SUP], Ye-Ji Lee[SUP] 1 [/SUP], Mi Seon Kim[SUP] 1 [/SUP], Yong-Pyo Lee[SUP] 1 [/SUP], Su-Jin Chae[SUP] 1 [/SUP], Kye Ryeong Park[SUP] 1 [/SUP], Seung-Rye Cho[SUP] 1 [/SUP], Sehee Park[SUP] 1 [/SUP], Su Jin Kim[SUP] 1 [/SUP], Eunbyeol Wang[SUP] 1 [/SUP], SangHee Woo[SUP] 1 [/SUP], Aram Lim[SUP] 1 [/SUP], Su-Jin Park[SUP] 1 [/SUP], JunHyeong Jang[SUP] 1 [/SUP], Yoon-Seok Chung[SUP] 1 [/SUP], Bum Sik Chin[SUP] 2 [/SUP], Jin-Soo Lee[SUP] 3 [/SUP], Duko Lim[SUP] 4 [/SUP], Myung-Guk Han[SUP] 1 [/SUP], Cheon Kwon Yoo[SUP] 5 [/SUP]
Affiliations
- PMID: 32528816
- PMCID: PMC7282421
- DOI: 10.24171/j.phrp.2020.11.3.02
Abstract
Objectives: Coronavirus Disease-19 (COVID-19) is a respiratory infection characterized by the main symptoms of pneumonia and fever. It is caused by the novel coronavirus severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2), which is known to spread via respiratory droplets. We aimed to determine the rate and likelihood of SARS-CoV-2 transmission from COVID-19 patients through non-respiratory routes.
Methods: Serum, urine, and stool samples were collected from 74 hospitalized patients diagnosed with COVID-19 based on the detection of SARS-CoV-2 in respiratory samples. The SARS-CoV-2 RNA genome was extracted from each specimen and real-time reverse transcription polymerase chain reaction performed. CaCo-2 cells were inoculated with the specimens containing the SARS-COV-2 genome, and subcultured for virus isolation. After culturing, viral replication in the cell supernatant was assessed.
Results: Of the samples collected from 74 COVID-19 patients, SARS-CoV-2 was detected in 15 serum, urine, or stool samples. The virus detection rate in the serum, urine, and stool samples were 2.8% (9/323), 0.8% (2/247), and 10.1% (13/129), and the mean viral load was 1,210 ? 1,861, 79 ? 30, and 3,176 ? 7,208 copy/μL, respectively. However, the SARS-CoV-2 was not isolated by the culture method from the samples that tested positive for the SARS-CoV-2 gene.
Conclusion: While the virus remained detectable in the respiratory samples of COVID-19 patients for several days after hospitalization, its detection in the serum, urine, and stool samples was intermittent. Since the virus could not be isolated from the SARS-COV-2-positive samples, the risk of viral transmission via stool and urine is expected to be low.
Keywords: COVID-19; SARS-CoV-2; transmission.