tetano
Editor, Senior Moderator
Biotechnol Lett. 2016 Jan 13. [Epub ahead of print]
[h=1]Oral vaccine of Lactococcus lactis harbouring pandemic H1N1 2009 haemagglutinin1 and nisP anchor fusion protein elevates anti-HA1 sIgA levels in mice.[/h] Joan SS[SUP]1[/SUP], Pui-Fong J[SUP]2,[/SUP][SUP]3[/SUP], Song AA[SUP]4[/SUP], Chang LY[SUP]2,[/SUP][SUP]3[/SUP], Yusoff K[SUP]5,[/SUP][SUP]6[/SUP], AbuBakar S[SUP]2,[/SUP][SUP]3[/SUP], Rahim RA[SUP]7,[/SUP][SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]OBJECTIVE:[/h] An oral lactococcal-based vaccine which haboured the haemagglutinin1 (HA1) antigen fused to nisP anchor protein for the purpose of surface displaying the HA1 antigen was developed against H1N1 virus.
[h=4]RESULTS:[/h] Recombinant L. lactis strains expressed HA1-nisP fusion proteins when induced with nisin, as confirmed through western blotting. However, immunofluorescense did not detect any surface-displayed proteins, suggesting that the protein was either unsuccessfully translocated or improperly displayed. Despite this, oral administration of recombinant L. lactis strains to BALB/c mice revealed that significant levels of anti-HA1 sIgA antibodies were detected in mice fecal suspension samples of mice group NZ9000 (pNZ:HN) when compared to the negative control NZ9000 (pNZ8048) group.
[h=4]CONCLUSION:[/h] Specific anti-HA1 sIgA antibodies were locally produced and live recombinant lactococcal vaccine was able to elicit humoral response of BALB/c mice despite unsuccessful surface display of the HA1 epitope.
[h=4]KEYWORDS:[/h] H1N1; Haemagglutinin; Influenza virus; Lactococcus lactis; Oral vaccine; Surface display; Vaccine
PMID: 26758876 [PubMed - as supplied by publisher]
[h=1]Oral vaccine of Lactococcus lactis harbouring pandemic H1N1 2009 haemagglutinin1 and nisP anchor fusion protein elevates anti-HA1 sIgA levels in mice.[/h] Joan SS[SUP]1[/SUP], Pui-Fong J[SUP]2,[/SUP][SUP]3[/SUP], Song AA[SUP]4[/SUP], Chang LY[SUP]2,[/SUP][SUP]3[/SUP], Yusoff K[SUP]5,[/SUP][SUP]6[/SUP], AbuBakar S[SUP]2,[/SUP][SUP]3[/SUP], Rahim RA[SUP]7,[/SUP][SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]OBJECTIVE:[/h] An oral lactococcal-based vaccine which haboured the haemagglutinin1 (HA1) antigen fused to nisP anchor protein for the purpose of surface displaying the HA1 antigen was developed against H1N1 virus.
[h=4]RESULTS:[/h] Recombinant L. lactis strains expressed HA1-nisP fusion proteins when induced with nisin, as confirmed through western blotting. However, immunofluorescense did not detect any surface-displayed proteins, suggesting that the protein was either unsuccessfully translocated or improperly displayed. Despite this, oral administration of recombinant L. lactis strains to BALB/c mice revealed that significant levels of anti-HA1 sIgA antibodies were detected in mice fecal suspension samples of mice group NZ9000 (pNZ:HN) when compared to the negative control NZ9000 (pNZ8048) group.
[h=4]CONCLUSION:[/h] Specific anti-HA1 sIgA antibodies were locally produced and live recombinant lactococcal vaccine was able to elicit humoral response of BALB/c mice despite unsuccessful surface display of the HA1 epitope.
[h=4]KEYWORDS:[/h] H1N1; Haemagglutinin; Influenza virus; Lactococcus lactis; Oral vaccine; Surface display; Vaccine
PMID: 26758876 [PubMed - as supplied by publisher]