Open Forum Infect Dis . Subgenomic RNA Abundance Relative to Total Viral RNA Among Severe Acute Respiratory Syndrome Coronavirus 2 Variants

[tetano]

Open Forum Infect Dis


. 2022 Nov 10;9(11)fac619.
doi: 10.1093/ofid/ofac619. eCollection 2022 Nov.
Subgenomic RNA Abundance Relative to Total Viral RNA Among Severe Acute Respiratory Syndrome Coronavirus 2 Variants


Maxwell Su[SUP] 1 [/SUP], Sara Ping[SUP] 1 [/SUP], Phuong-Vi Nguyen[SUP] 1 [/SUP], Alejandra Rojas[SUP] 2 [/SUP], Laila Hussaini[SUP] 3 [/SUP], Ludy Registre Carmola[SUP] 4 [/SUP], Azmain Taz[SUP] 4 [/SUP], Julie Sullivan[SUP] 5 [/SUP], Greg S Martin[SUP] 5 6 [/SUP], Anne Piantadosi[SUP] 1 4 5 [/SUP], Magaly Martinez[SUP] 7 [/SUP], Wilbur A Lam[SUP] 3 5 8 [/SUP], Evan J Anderson[SUP] 1 3 [/SUP], Jesse J Waggoner[SUP] 1 5 9 [/SUP]



Affiliations

• PMID: 36467291
• PMCID: PMC9709700
• DOI: 10.1093/ofid/ofac619

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) subgenomic RNA (sgRNA) may indicate actively replicating virus, but sgRNA abundance has not been systematically compared between SARS-CoV-2 variants. sgRNA was quantified in 169 clinical samples by real-time reverse-transcription polymerase chain reaction, demonstrating similar relative abundance among known variants. Thus, sgRNA detection can identify individuals with active viral replication regardless of variant.

Keywords: severe acute respiratory syndrome coronavirus 2; subgenomic RNA; variants of concern.