tetano
Editor, Senior Moderator
Open Forum Infect Dis
. 2024 Feb 2;11(3)
fae040.
doi: 10.1093/ofid/ofae040. eCollection 2024 Mar. Healthcare Provider N95 Respirator Contamination Worn Behind Face Shields With SARS-CoV-2 During Routine Clinical Care of Patients With COVID-19
Amanda M Graves[SUP] 1 2 3 [/SUP], Bobby G Warren[SUP] 1 2 3 [/SUP], Aaron Barrett[SUP] 1 2 3 [/SUP], Sarah S Lewis[SUP] 1 2 [/SUP], Becky Smith[SUP] 1 2 [/SUP], David J Weber[SUP] 4 [/SUP], Emily E Sickbert-Bennett[SUP] 4 [/SUP], Deverick J Anderson[SUP] 1 2 3 [/SUP]
Affiliations
N95 respirator contamination with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) during clinical care of patients with coronavirus disease 2019 is poorly understood. We performed a prospective observational study on healthcare provider's (HCP's) N95 respirators' and face shields' SARS-CoV-2 contamination during aerosol-generating procedures on SARS-CoV-2-positive patients housed in a COVID-19-specific unit. Medical masks worn on top of HCP's N95 respirators, and under face shields, during study aerosol-generating procedures were used as surrogates to detect contamination to avoid waste. Thirty-three HCPs were studied, and a total of 33 mask and 27 face shields were sampled. Masks were cut into 9 pieces and face shields were sampled twice, front and back, to determine locality of contamination; however, no positive samples were identified using standard polymerase chain reaction techniques with a CT value up to 40. All 9 mask piece samples were then pooled, as were face shield samples, using centrifugal concentration with polyethersulfone membranes. Once pooled and concentrated, overall, 9 (15%) samples were positive via real-time polymerase chain reaction: 5 from masks (15.2%) and 4 from face shields (14.8%).
Keywords: SARS-CoV-2; healthcare epidemiology; hospital environment; mask contamination.
. 2024 Feb 2;11(3)
fae040.doi: 10.1093/ofid/ofae040. eCollection 2024 Mar. Healthcare Provider N95 Respirator Contamination Worn Behind Face Shields With SARS-CoV-2 During Routine Clinical Care of Patients With COVID-19
Amanda M Graves[SUP] 1 2 3 [/SUP], Bobby G Warren[SUP] 1 2 3 [/SUP], Aaron Barrett[SUP] 1 2 3 [/SUP], Sarah S Lewis[SUP] 1 2 [/SUP], Becky Smith[SUP] 1 2 [/SUP], David J Weber[SUP] 4 [/SUP], Emily E Sickbert-Bennett[SUP] 4 [/SUP], Deverick J Anderson[SUP] 1 2 3 [/SUP]
Affiliations
- PMID: 38449922
- PMCID: PMC10917086
- DOI: 10.1093/ofid/ofae040
N95 respirator contamination with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) during clinical care of patients with coronavirus disease 2019 is poorly understood. We performed a prospective observational study on healthcare provider's (HCP's) N95 respirators' and face shields' SARS-CoV-2 contamination during aerosol-generating procedures on SARS-CoV-2-positive patients housed in a COVID-19-specific unit. Medical masks worn on top of HCP's N95 respirators, and under face shields, during study aerosol-generating procedures were used as surrogates to detect contamination to avoid waste. Thirty-three HCPs were studied, and a total of 33 mask and 27 face shields were sampled. Masks were cut into 9 pieces and face shields were sampled twice, front and back, to determine locality of contamination; however, no positive samples were identified using standard polymerase chain reaction techniques with a CT value up to 40. All 9 mask piece samples were then pooled, as were face shield samples, using centrifugal concentration with polyethersulfone membranes. Once pooled and concentrated, overall, 9 (15%) samples were positive via real-time polymerase chain reaction: 5 from masks (15.2%) and 4 from face shields (14.8%).
Keywords: SARS-CoV-2; healthcare epidemiology; hospital environment; mask contamination.