tetano
Editor, Senior Moderator
Open Forum Infect Dis
. 2025 Jan 16;12(1)
faf007.
doi: 10.1093/ofid/ofaf007. eCollection 2025 Jan. BCG-Induced DNA Methylation Changes Improve Coronavirus Disease 2019 Vaccine Immunity Without Decreasing the Risk for Severe Acute Respiratory Syndrome Coronavirus 2 Infection
Santiago Carrero Longlax[SUP] 1 [/SUP], Kent J Koster[SUP] 2 [/SUP], Ashish M Kamat[SUP] 3 [/SUP], Marisa Lozano[SUP] 3 [/SUP], Seth P Lerner[SUP] 4 [/SUP], Rebecca Hannigan[SUP] 4 [/SUP], Tomoki Nishiguchi[SUP] 1 [/SUP], Abhimanyu[SUP] 1 [/SUP], Daanish Sheikh[SUP] 1 [/SUP], Malik Ladki[SUP] 1 [/SUP], Alexandra Portillo[SUP] 1 [/SUP], Amrit Koirala[SUP] 5 [/SUP], Tajhal D Patel[SUP] 5 [/SUP], Zoe Spieler[SUP] 1 [/SUP], Aaron B Benjamin[SUP] 2 [/SUP], Maxim Lebedev[SUP] 2 [/SUP], Theresa U Ofili[SUP] 6 [/SUP], Robert W Hutchison[SUP] 6 [/SUP], George Udeani[SUP] 6 [/SUP], Lynne A Opperman[SUP] 7 [/SUP], Gabriel Neal[SUP] 8 [/SUP], Anna M Mandalakas[SUP] 1 9 10 [/SUP], Mihai G Netea[SUP] 11 [/SUP], Moshe Arditi[SUP] 12 [/SUP], Pablo Avalos[SUP] 12 [/SUP], Sandra L Grimm[SUP] 5 13 [/SUP], Cristian Coarfa[SUP] 5 13 [/SUP], Jeffrey D Cirillo[SUP] 2 [/SUP], Andrew R DiNardo[SUP] 1 11 [/SUP]
Affiliations
Background: The BCG vaccine induces trained immunity, an epigenetic-mediated increase in innate immune responsiveness. Therefore, this clinical trial evaluated if BCG-induced trained immunity could decrease coronavirus disease 2019 (COVID-19)-related frequency or severity.
Methods: A double-blind, placebo-controlled clinical trial of healthcare workers randomized participants to vaccination with BCG TICE or placebo (saline). Enrollment included 529 healthcare workers randomized to receive BCG or placebo. Primary analysis evaluated COVID-19 disease frequency, while secondary analysis evaluated coronavirus immunity in a subset of participants. Study enrollment ceased early in December 2020 following introduction of COVID-19-specific vaccines.
Results: Study enrollment was halted early, prior to reaching the targeted recruitment, and was not powered to detect a decrease in COVID-19 frequency. Symptomatic COVID-19 occurred in 21 of 263 and 10 of 266 participants in the BCG and placebo arms, respectively (P = .50, Fisher exact test). Participants vaccinated with BCG, but uninfected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), demonstrated increased coronavirus vaccine immunity (increase spike-inducible levels of tumor necrosis factor, interleukin 6, and interleukin 1β) 12 months after BCG vaccination compared to participants receiving placebo. Immune responsiveness to SARS-CoV-2 antigens correlated with BCG-induced DNA methylation changes.
Conclusions: Due to early study closure, the study was not powered to evaluate COVID-19 frequency. Secondary analysis demonstrated that 12 months following vaccination, BCG increased coronavirus vaccine immunity compared to those who did not receive BCG. This increase in COVID-19 vaccine immunity correlated with BCG-induced DNA methylation changes.
Keywords: BCG vaccine; COVID-19; DNA methylation; epigenetics; innate training.
. 2025 Jan 16;12(1)
faf007.doi: 10.1093/ofid/ofaf007. eCollection 2025 Jan. BCG-Induced DNA Methylation Changes Improve Coronavirus Disease 2019 Vaccine Immunity Without Decreasing the Risk for Severe Acute Respiratory Syndrome Coronavirus 2 Infection
Santiago Carrero Longlax[SUP] 1 [/SUP], Kent J Koster[SUP] 2 [/SUP], Ashish M Kamat[SUP] 3 [/SUP], Marisa Lozano[SUP] 3 [/SUP], Seth P Lerner[SUP] 4 [/SUP], Rebecca Hannigan[SUP] 4 [/SUP], Tomoki Nishiguchi[SUP] 1 [/SUP], Abhimanyu[SUP] 1 [/SUP], Daanish Sheikh[SUP] 1 [/SUP], Malik Ladki[SUP] 1 [/SUP], Alexandra Portillo[SUP] 1 [/SUP], Amrit Koirala[SUP] 5 [/SUP], Tajhal D Patel[SUP] 5 [/SUP], Zoe Spieler[SUP] 1 [/SUP], Aaron B Benjamin[SUP] 2 [/SUP], Maxim Lebedev[SUP] 2 [/SUP], Theresa U Ofili[SUP] 6 [/SUP], Robert W Hutchison[SUP] 6 [/SUP], George Udeani[SUP] 6 [/SUP], Lynne A Opperman[SUP] 7 [/SUP], Gabriel Neal[SUP] 8 [/SUP], Anna M Mandalakas[SUP] 1 9 10 [/SUP], Mihai G Netea[SUP] 11 [/SUP], Moshe Arditi[SUP] 12 [/SUP], Pablo Avalos[SUP] 12 [/SUP], Sandra L Grimm[SUP] 5 13 [/SUP], Cristian Coarfa[SUP] 5 13 [/SUP], Jeffrey D Cirillo[SUP] 2 [/SUP], Andrew R DiNardo[SUP] 1 11 [/SUP]
Affiliations
- PMID: 39872813
- PMCID: PMC11770274
- DOI: 10.1093/ofid/ofaf007
Background: The BCG vaccine induces trained immunity, an epigenetic-mediated increase in innate immune responsiveness. Therefore, this clinical trial evaluated if BCG-induced trained immunity could decrease coronavirus disease 2019 (COVID-19)-related frequency or severity.
Methods: A double-blind, placebo-controlled clinical trial of healthcare workers randomized participants to vaccination with BCG TICE or placebo (saline). Enrollment included 529 healthcare workers randomized to receive BCG or placebo. Primary analysis evaluated COVID-19 disease frequency, while secondary analysis evaluated coronavirus immunity in a subset of participants. Study enrollment ceased early in December 2020 following introduction of COVID-19-specific vaccines.
Results: Study enrollment was halted early, prior to reaching the targeted recruitment, and was not powered to detect a decrease in COVID-19 frequency. Symptomatic COVID-19 occurred in 21 of 263 and 10 of 266 participants in the BCG and placebo arms, respectively (P = .50, Fisher exact test). Participants vaccinated with BCG, but uninfected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), demonstrated increased coronavirus vaccine immunity (increase spike-inducible levels of tumor necrosis factor, interleukin 6, and interleukin 1β) 12 months after BCG vaccination compared to participants receiving placebo. Immune responsiveness to SARS-CoV-2 antigens correlated with BCG-induced DNA methylation changes.
Conclusions: Due to early study closure, the study was not powered to evaluate COVID-19 frequency. Secondary analysis demonstrated that 12 months following vaccination, BCG increased coronavirus vaccine immunity compared to those who did not receive BCG. This increase in COVID-19 vaccine immunity correlated with BCG-induced DNA methylation changes.
Keywords: BCG vaccine; COVID-19; DNA methylation; epigenetics; innate training.