tetano
Editor, Senior Moderator
One Health Outlook
. 2022 Jun 24;4(1):12.
doi: 10.1186/s42522-022-00068-9.
Protective efficacy of an RBD-based Middle East respiratory syndrome coronavirus (MERS-CoV) particle vaccine in llamas
Jordi Rodon[SUP] 1 2 [/SUP], Anna Z Mykytyn[SUP] 3 [/SUP], Guillermo Cantero[SUP] 1 2 [/SUP], Irina C Albulescu[SUP] 4 [/SUP], Berend-Jan Bosch[SUP] 4 [/SUP], Alexander Brix[SUP] 5 [/SUP], Jean-Christophe Audonnet[SUP] 6 [/SUP], Albert Bensaid[SUP] 1 2 [/SUP], Júlia Vergara-Alert[SUP] 1 2 [/SUP], Bart L Haagmans[SUP] 7 [/SUP], Joaquim Segalés[SUP] 8 9 [/SUP]
Affiliations
Abstract
Ongoing outbreaks of Middle East respiratory syndrome coronavirus (MERS-CoV) continue posing a global health threat. Vaccination of livestock reservoir species is a recommended strategy to prevent spread of MERS-CoV among animals and potential spillover to humans. Using a direct-contact llama challenge model that mimics naturally occurring viral transmission, we tested the efficacy of a multimeric receptor binding domain (RBD) particle-display based vaccine candidate. While MERS-CoV was transmitted to naïve animals exposed to virus-inoculated llamas, immunization induced robust virus-neutralizing antibody responses and prevented transmission in 1/3 vaccinated, in-contact animals. Our exploratory study supports further improvement of the RBD-based vaccine to prevent zoonotic spillover of MERS-CoV.
Keywords: Animal model; Camelid; Llama; MERS-CoV; Middle East respiratory syndrome coronavirus; Multimeric protein scaffold particles (MPSP); Neutralizing antibodies; Receptor binding domain (RBD)-based vaccine; Virus transmission.
. 2022 Jun 24;4(1):12.
doi: 10.1186/s42522-022-00068-9.
Protective efficacy of an RBD-based Middle East respiratory syndrome coronavirus (MERS-CoV) particle vaccine in llamas
Jordi Rodon[SUP] 1 2 [/SUP], Anna Z Mykytyn[SUP] 3 [/SUP], Guillermo Cantero[SUP] 1 2 [/SUP], Irina C Albulescu[SUP] 4 [/SUP], Berend-Jan Bosch[SUP] 4 [/SUP], Alexander Brix[SUP] 5 [/SUP], Jean-Christophe Audonnet[SUP] 6 [/SUP], Albert Bensaid[SUP] 1 2 [/SUP], Júlia Vergara-Alert[SUP] 1 2 [/SUP], Bart L Haagmans[SUP] 7 [/SUP], Joaquim Segalés[SUP] 8 9 [/SUP]
Affiliations
- PMID: 35739576
- DOI: 10.1186/s42522-022-00068-9
Abstract
Ongoing outbreaks of Middle East respiratory syndrome coronavirus (MERS-CoV) continue posing a global health threat. Vaccination of livestock reservoir species is a recommended strategy to prevent spread of MERS-CoV among animals and potential spillover to humans. Using a direct-contact llama challenge model that mimics naturally occurring viral transmission, we tested the efficacy of a multimeric receptor binding domain (RBD) particle-display based vaccine candidate. While MERS-CoV was transmitted to naïve animals exposed to virus-inoculated llamas, immunization induced robust virus-neutralizing antibody responses and prevented transmission in 1/3 vaccinated, in-contact animals. Our exploratory study supports further improvement of the RBD-based vaccine to prevent zoonotic spillover of MERS-CoV.
Keywords: Animal model; Camelid; Llama; MERS-CoV; Middle East respiratory syndrome coronavirus; Multimeric protein scaffold particles (MPSP); Neutralizing antibodies; Receptor binding domain (RBD)-based vaccine; Virus transmission.