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Officials discuss animal die-off response

Re: Officials discuss animal die-off response

niman said:
It looks like earlier story is available for a fee


<TABLE cellSpacing=0 cellPadding=0 width="90%" align=center border=0><TBODY><TR><TD>[FONT=Arial,Helvetica][SIZE=+1]Understanding the duck deaths[/SIZE][/FONT] </TD></TR><TR><TD bgColor=#ffffff height=6>
spacer.gif
</TD></TR><TR><TD><TABLE cellSpacing=0 cellPadding=0 width="90%" align=center border=0><TBODY><TR><TD>[FONT=Arial,Helvetica][SIZE=-1]Author: From staff reports
Date: September 2, 2006
Publication: Lake County Record Bee (Lakeport, CA)
[/SIZE][/FONT]
</TD></TR><TR><TD>[FONT=Arial,Helvetica][SIZE=-1]Cynthia Davis -- Record-Bee staff LAKEPORT -- The city of Lakeport's new Web site currently lists "Dead Birds at Library Park" under the category of Hot Topics for residents.
It's an apt description at a time when the heat is on the city and county to quickly respond to dead or dying ducks in the city's centerpiece park.
The waterfowl are believed to be affected by avian botulism, but the actual cause may never be known for........... [/SIZE][/FONT]
</TD></TR></TBODY></TABLE></TD></TR></TBODY></TABLE>

More from keyword

Understanding the duck deaths
[SIZE=-1]Lake County Record-Bee, CA - <NOBR>Sep 2, 2006</NOBR>[/SIZE]
[SIZE=-1]... The waterfowl are believed to be affected by avian botulism, but the actual cause may never be known for certain due to a miscommunication in regard to the ...[/SIZE]
 
Re: Officials discuss animal die-off response

Looks like two stories on August 29 by two reporters

<TABLE cellSpacing=7 cellPadding=2 width="75%" border=0 valign="top"><TBODY><TR><TD vAlign=top>Disposal remains unclear for ducks
[SIZE=-1]Lake County Record-Bee, CA - <NOBR>Aug 29, 2006</NOBR>[/SIZE]
[SIZE=-1]LAKEPORT -- Monday the Fish and Game Wildlife Investigation Lab confirmed a presumptive diagnosis of avian botulism in recent Library Park duck deaths. ... [/SIZE]
</TD></TR></TBODY></TABLE><TABLE cellSpacing=7 cellPadding=2 width="75%" border=0 valign="top"><TBODY><TR><TD vAlign=top>No one's in charge ... that's the problem
[SIZE=-1]Lake County Record-Bee, CA - <NOBR>Aug 29, 2006</NOBR>[/SIZE]
[SIZE=-1]By Terry Knight -- Record-Bee outdoors columnist. The recent death of approximately a dozen wild ducks at Library Park in Lakeport is nothing new. ... [/SIZE]</TD></TR></TBODY></TABLE>
 
Re: Officials discuss animal die-off response

More search results

Disposal remains unclear for ducks
[SIZE=-1]Lake County Record-Bee, CA - <NOBR>Aug 29, 2006</NOBR>[/SIZE]
[SIZE=-1]... of Lakeport have now been advised by Associate Wildlife Biologist of the California Dept of Fish and Game Allan Buckman to create a cooperative rescue program ... [/SIZE]
 
Re: Officials discuss animal die-off response

niman said:
I don't see any inconsistancies. Over the summer there was a duck die off (maybe 50 ducks). The diagnosis was botulism. Now there is a least one more (but probably more) who died in late September. I suspect the H5 is from the more recent case.

USDA hasn't said anything on these birds, at least not recently.

H5 in a dead duck is not a good indicator. The earlier die off involved bent neck.
Is this about the earlier die off you talk about ?
http://www.newsobserver.com/102/story/493089.html

Published: Oct 01, 2006 12:30 AM
Modified: Oct 01, 2006 02:57 AM
Bacterium caused duck deaths

Jerry Allegood, Staff Writer
SWANSBORO - A common bacterium is to blame for mysterious illnesses and deaths among Swansboro's free-roaming ducks, according to the N.C. Department of Agriculture.Mary Ann McBride, assistant state veterinarian, said tests confirmed the ducks died of avian botulism, a paralytic disease caused by ingestion of a toxin or poison produced by a bacterium commonly found in soil and stagnant water. Birds sometimes ingest the toxin directly while foraging or may eat toxin-laden maggots.
McBride said the outbreak did not pose a public health hazard.
Dozens of Muscovy ducks that waddled around the waterfront died in July and August, prompting some to suggest the ducks had been intentionally poisoned. Town officials said they did nothing to destroy the ducks, which are characterized by a red crest above the beaks of adults.
State officials earlier ruled out avian bird flu and exotic Newcastle disease and said there was no indication of an infectious disease that would threaten poultry or humans. More detailed tests that can take weeks were required for botulism, according to McBride, who said the investigation ended with the conclusive results on Friday.
"It was unfortunate for the ducks, but at least we know what we are dealing with," she said.
Botulism in humans usually results from eating improperly canned foods.

Staff writer Jerry Allegood can be reached in Greenville at (252) 752-8411 or jerrya@newsobserver.com.
 
Re: Officials discuss animal die-off response

Mingus said:
Is this about the earlier die off you talk about ?

No, the earlier die-off was at the same location in Lakeport, CA.

The report you listed was in North Carolina.

There have been several recent die-offs linked to avian botulism - Lakeport, CA, Lake Erie (where H5N1 was later confirmed in mute swans Michigan and mallards in Pennsylvania) and North Carolina.
 
Re: Officials discuss animal die-off response

PonyGirl said:
H5 in a dead duck is not a good indicator. The earlier die off involved bent neck.

Am curious as to where this info came from.

Also looking at this:

http://www.recombinomics.com/News/11210502/H5_Duck_Fatal_BC.html

Several botulism or poisoning die-off stories on dead waterfowl mention bent necks. I thought one of the stories was from California, but haven't found it yet with searches. A few more hits are:

No one's in charge ... that's the problem
[SIZE=-1]Lake County Record-Bee, CA - <NOBR>Aug 29, 2006</NOBR>[/SIZE]
[SIZE=-1]... by forcing fresh water down its throat to purge its system. According to DFG wildlife biologist Alan Buckmann, the dead ducks at Library Park were probably ... [/SIZE]

No one's in charge ... that's the problem
[SIZE=-1]Lake County Record-Bee, CA - <NOBR>Aug 29, 2006</NOBR>[/SIZE]
[SIZE=-1]... According to DFG wildlife biologist Alan Buckmann, the dead ducks at Library Park were probably caused by several infected birds that arrived and died at the ... [/SIZE]

Disposal remains unclear for ducks
[SIZE=-1]Lake County Record-Bee, CA - <NOBR>Aug 29, 2006</NOBR>[/SIZE]
[SIZE=-1]... Lab confirmed a presumptive diagnosis of avian botulism in recent Library Park duck deaths ... The ducks were found dead along the shoreline in the park last Friday ... [/SIZE]

<TABLE cellSpacing=7 cellPadding=2 width="75%" border=0 valign="top"><TBODY><TR><TD vAlign=top>Understanding the duck deaths
[SIZE=-1]Lake County Record-Bee, CA - <NOBR>Sep 2, 2006</NOBR>[/SIZE]
[SIZE=-1]... "We are checking Library Park at least three ... in place, that's why we called the numbers we did and the health department last Friday, and Richard Knoll is ... [/SIZE]

</TD></TR></TBODY></TABLE><TABLE cellSpacing=7 cellPadding=2 width="75%" border=0 valign="top"><TBODY><TR><TD vAlign=top>Disposal remains unclear for ducks
[SIZE=-1]Lake County Record-Bee, CA - <NOBR>Aug 29, 2006</NOBR>[/SIZE]
[SIZE=-1]... diagnosis of avian botulism in recent Library Park duck deaths. ... along the shoreline in the park last Friday. ... with Interim Acting City Manager Richard Knoll and ... [/SIZE]
</TD></TR></TBODY></TABLE>

Disposal remains unclear for ducks
[SIZE=-1]Lake County Record-Bee, CA - <NOBR>Aug 29, 2006</NOBR>[/SIZE]
[SIZE=-1]... Lab confirmed a presumptive diagnosis of avian botulism in recent Library Park duck deaths. ... ducks were found dead along the shoreline in the park last Friday. ... [/SIZE]

<TABLE cellSpacing=7 cellPadding=2 width="75%" border=0 valign="top"><TBODY><TR><TD vAlign=top>Understanding the duck deaths
[SIZE=-1]Lake County Record-Bee, CA - <NOBR>Sep 2, 2006</NOBR>[/SIZE]
[SIZE=-1]... "We are checking Library Park at least three ... in place, that's why we called the numbers we did and the health department last Friday, and Richard Knoll is now ... [/SIZE]

</TD></TR></TBODY></TABLE><TABLE cellSpacing=7 cellPadding=2 width="75%" border=0 valign="top"><TBODY><TR><TD vAlign=top>Disposal remains unclear for ducks
[SIZE=-1]Lake County Record-Bee, CA - <NOBR>Aug 29, 2006</NOBR>[/SIZE]
[SIZE=-1]... According to Knoll, the city recommends that anyone finding a dead duck on the lakeshore area of Library Park/Lakefront Park call the Lakeport Public Works ... [/SIZE]
</TD></TR></TBODY></TABLE>
 
Re: Officials discuss animal die-off response

More bits and pieces

<TABLE cellSpacing=7 cellPadding=2 width="75%" border=0 valign="top"><TBODY><TR><TD vAlign=top>Understanding the duck deaths
[SIZE=-1]Lake County Record-Bee, CA - <NOBR>Sep 2, 2006</NOBR>[/SIZE]
[SIZE=-1]... Doug Grider, the city of Lakeport's public works superintendent, said that, despite what has caused the duck die-offs ... "We are checking Library Park at least ... [/SIZE]
</TD></TR></TBODY></TABLE><TABLE cellSpacing=7 cellPadding=2 width="75%" border=0 valign="top"><TBODY><TR><TD vAlign=top>No one's in charge ... that's the problem
[SIZE=-1]Lake County Record-Bee, CA - <NOBR>Aug 29, 2006</NOBR>[/SIZE]
[SIZE=-1]... In the duck incident at Library Park no warning was given to the public about ... the County Health Department, Vector Control or the County Public Works responded ... [/SIZE]</TD></TR></TBODY></TABLE>

<TABLE cellSpacing=0 cellPadding=0 width="100%" border=0><TBODY><TR><TD align=left><TABLE cellSpacing=7 cellPadding=2 width="75%" border=0 valign="top"><TBODY><TR><TD vAlign=top>No one's in charge ... that's the problem
[SIZE=-1]Lake County Record-Bee, CA - <NOBR>Aug 29, 2006</NOBR>[/SIZE]
[SIZE=-1]... incident at Library Park no warning was given to the public about not handling the dead birds unless precautions are taken -- such as wearing latex gloves and ... [/SIZE]
</TD></TR></TBODY></TABLE><TABLE cellSpacing=7 cellPadding=2 width="75%" border=0 valign="top"><TBODY><TR><TD vAlign=top>Disposal remains unclear for ducks
[SIZE=-1]Lake County Record-Bee, CA - <NOBR>Aug 29, 2006</NOBR>[/SIZE]
[SIZE=-1]... that anyone finding a dead duck on the lakeshore area of Library Park/Lakefront Park ... to help stop the spread of the botulism and for them to wear gloves or use ... [/SIZE]</TD></TR></TBODY></TABLE></TD></TR></TBODY></TABLE>
<TABLE cellSpacing=7 cellPadding=2 width="75%" border=0 valign="top"><TBODY><TR><TD vAlign=top>Understanding the duck deaths
[SIZE=-1]Lake County Record-Bee, CA - <NOBR>Sep 2, 2006</NOBR>[/SIZE]
[SIZE=-1]... said that, despite what has caused the duck die-offs, the city staff is working hard on the cleanup efforts. "We are checking Library Park at least three times ... [/SIZE]
</TD></TR></TBODY></TABLE><TABLE cellSpacing=7 cellPadding=2 width="75%" border=0 valign="top"><TBODY><TR><TD vAlign=top>No one's in charge ... that's the problem
[SIZE=-1]Lake County Record-Bee, CA - <NOBR>Aug 29, 2006</NOBR>[/SIZE]
[SIZE=-1]... When large die-offs occur in the Sacramento Valley, the Department of Fish ... DFG wildlife biologist Alan Buckmann, the dead ducks at Library Park were probably ... [/SIZE]</TD></TR></TBODY></TABLE>
 
Re: Officials discuss animal die-off response

I still have not heard from the newspaper. Tomorrow I will be contacting them again and several officials.
 
Re: Officials discuss animal die-off response

The testing was not performed by the USDA. But by the Califorina dept of fish and wildlife. Only when tests are positive for H5 or H7 are samples sent to Aims Iowa for "The test". Because that requires Growing the virus in at least a Bio-level III lab.

One Duck only?
 
Re: Officials discuss animal die-off response

Sonny said:
The testing was not performed by the USDA. But by the Califorina dept of fish and wildlife. Only when tests are positive for H5 or H7 are samples sent to Aims Iowa for "The test". Because that requires Growing the virus in at least a Bio-level III lab.

One Duck only?

I think most of the ducks died in August. Something like 50 ducks died then and at least one was positive for botulism. It is not clear how many of the earlier ducks were tested for avian influenza, since botulism was detected.

More ducks have died recently. At least one of the recent dead birds was H5 positive. Samples were sent to Ames, but they have yet to comment. At least one more duck has died recently, but there is no data on how many have been tested for avian influenza, and there is no indication that the recent dead ducks were positive for botulism, since there were no dead duck deaths in early September.
 
Re: Officials discuss animal die-off response

niman said:
I don't see any inconsistancies. Over the summer there was a duck die off (maybe 50 ducks). The diagnosis was botulism. Now there is a least one more (but probably more) who died in late September. I suspect the H5 is from the more recent case.

USDA hasn't said anything on these birds, at least not recently.

H5 in a dead duck is not a good indicator. The earlier die off involved bent neck.

As an exercise in humility I checked a number of cooperative extension webpages to find out what twisted/bent necks in birds might be a symptom of. I came up with an extensive list:

Newcastle disease, fowl cholera, brain infections, avian influenza (not limited to H5N1), paratyphoid, nutritional deficiencies (usu. vitamin B1), head trauma, heat stroke-induced nerve damage, west nile virus...

And from the FAO site on diseases in livestock:
http://www.fao.org/docrep/008/y5169e/y5169e07.htm

"Ingestion of toxic plant parts (such as leaves, seeds and sap) is a common hazard for free-range birds. Some toxins are produced by micro-organisms, such as those liberated by the bacteria Clostridium botulinum and C. perfringens, both found in the soil. C. perfringens causes necrotic enteritis, caused when the bacteria multiplies in the favourable conditions of the digestive tract and liberates a potent toxin that results in high mortality. Occasionally affected birds show anorexia, depression and diarrhoea, but most die without showing any clinical signs. C. botulinum causes botulism disease, which is acute food poisoning. This is more common in ducks, which show the nervous symptoms of neck bent down and feathers falling out easily when lightly pulled. Botulism results from the bird eating rotting vegetable scraps, which contain the toxins produced by the C. botulinum. Household vegetable scraps which are not regularly removed are a potential hazard for botulism." [emphasis added

Seems like a good reason to potentially not feed scraps to ducks as well...
 
Re: Officials discuss animal die-off response

canagica said:
As an exercise in humility I checked a number of cooperative extension webpages to find out what twisted/bent necks in birds might be a symptom of. I came up with an extensive list:

Newcastle disease, fowl cholera, brain infections, avian influenza (not limited to H5N1), paratyphoid, nutritional deficiencies (usu. vitamin B1), head trauma, heat stroke-induced nerve damage, west nile virus...

And from the FAO site on diseases in livestock:
http://www.fao.org/docrep/008/y5169e/y5169e07.htm

"Ingestion of toxic plant parts (such as leaves, seeds and sap) is a common hazard for free-range birds. Some toxins are produced by micro-organisms, such as those liberated by the bacteria Clostridium botulinum and C. perfringens, both found in the soil. C. perfringens causes necrotic enteritis, caused when the bacteria multiplies in the favourable conditions of the digestive tract and liberates a potent toxin that results in high mortality. Occasionally affected birds show anorexia, depression and diarrhoea, but most die without showing any clinical signs. C. botulinum causes botulism disease, which is acute food poisoning. This is more common in ducks, which show the nervous symptoms of neck bent down and feathers falling out easily when lightly pulled. Botulism results from the bird eating rotting vegetable scraps, which contain the toxins produced by the C. botulinum. Household vegetable scraps which are not regularly removed are a potential hazard for botulism." [emphasis added

Seems like a good reason to potentially not feed scraps to ducks as well...

The bent neck is a neurological indocation, and H5N1 frequently causes bent neck in waterfowl. It remains unclear how many of the earlier cases were tested for avian influenza, since botulism was diagnosed.

In the later cases, at least one was positive for H5. Botulism has not been mentioned for the later cases. There seems to be a time gap of two or more weeks between the first die-off, which involved something like 50 ducks, and the more recent H5 positive die-off, which may have only involved a few ducks.

H5N1 can infected many species, and in many cases the waterfowl are asymptomatic and just have H5N1 antibodies.

LPAI and HPAI refer to the ability to avian influenza to kill experimental chickens. The same H5N1 that kills chickens (and humans), may produce a mild or no disease in waterfowl, which is why WHO issued its warning in late 2004 (H5N1 from dead patients produce mild disease in waterfowl, although high levels of H5N1 were in the gut and excreted in feces).
 
Last edited:
Re: Officials discuss animal die-off response

I spoke to Allan Buckman and there appear to be some inconsistencies. He was very gracious. He was unaware of this article in the Record-Bee. I am calling Alexia Retallack now. She is at the state level and may have more information to add to Allan's.

I will post my findings this afternoon.
 
Re: Officials discuss animal die-off response

Article Last Updated: 10/02/2006 06:10:58 AM PDT
http://www.record-bee.com/local/ci_4422654

Officials discuss animal die-off response
By Cynthia Davis -- Record-Bee staff

LAKEPORT -- The City Council will soon discuss banning any feeding of ducks or waterfowl at Library Park. This comes after another dead duck was found last weekend at Library Park and one of the ducks tested previously by the California Department of Fish and Game has tested positive for H5 avian influenza.

According to officials at the California Department of Fish and Game, further testing is now being done to determine the exact avian influenza subtype.

Alexia Retallack, a California Fish and Game information officer, said that all indications show that this is a low-pathogen strain of bird flu that is not a threat to human health.

"In this particular case, we had a positive hit for avian influenza and the subtype is H5 category," said Retallack.

"There are 16 h types and 9 N types which can mix together into 144 different combinations, most of which are no problem," Retallack added, "So when you get an H5 you send it in for further testing to determine the N type. So far it looks like there is nothing to worry about."

The duck is undergoing further testing at the USDA's National Veterinary Services Laboratory in Ames, Iowa, said Retallack. The lab would have notified them if the sub type was N1 or something to be concerned about, she added.

"The most important thing they were able to figure out is that it was low pathogen," said Fish and Game biologist, Patrick Foy.

USGS Office of Communications spokesperson Catherine Puckett said, "So far, in North America, we have not found the virus strain of concern (HPAI or Highly Pathogenic Avian Influenza H5N1) worldwide in any of the thousands of ducks and other birds we have tested. As far as avian influenza is concerned, waterfowl and shorebirds commonly carry avian influenza (mostly strains that are not threatening to human and poultry health), and thus, researchers expect to find different kinds of avian influenza when they conduct tests of live or dead waterfowl."

Given that the avian influenza strain identified in the local duck is low pathogen, it is not to be confused with the bird flu strain causing concern around the globe, called H5N1.

Since it first appeared in Hong Kong in 1997, the H5N1 virus has caused 208 reported cases of human illness, including 115 deaths, according to the World Health Organization. In nearly all cases, the illnesses resulted from extensive, direct contact with infected birds. H5N1 is a bird virus and rarely affects humans. It has spread through parts of Europe, Asia and Africa, but has not yet been found in the Americas.

Bush administration health officials said last March that H5N1 is likely to arrive in this country this year.

The concern locally is how to address duck die-offs and collect dead animals in a timely manner.

Lakeport Acting City Manager Richard Knoll said the city has developed
an internal protocol, titled, "Responding to dead or dying fish, birds, or other animals."

"We are in the early stages of developing a plan to address the human influenza," said Knoll. "I think it's important to understand the potential issues. For me as a lay person I don't fully understand the potential, but some are saying it's not a matter of if, but when."

He added, "Eventually, this will be a regional issue."

Fish and Game biologist Allan Buckman said he is working on a cooperative disease response program for Lake County, the City of Lakeport, and other cities around the county. Buckman said he also has discussed the issue of the potential for disease from domestic waterfowl in Library Park with Knoll.

Buckman said he recommends removal of all domestics, prohibition of the public dumping of birds there and termination of feeding.

In an e-mail sent Thursday to the Record-Bee, Buckman noted, "I am thinking of similar situations where a small feeding zoo' was established near human use areas for people who want to feed such animals, if that remains a local concern. I am pleased they are taking this seriously."

If the ordinance is by the city, said Buckman, then they would administer their own laws. "I am suggesting the same concerns to the county and other cities on other lake front parks, but this needs to be developed as one large coordinated plan."

Retallack reiterated Buckman's statements, saying "Any AI H5N1 high pathogen event will trigger a response from both the federal and state agencies and we would bring in USDA, CA Department of Food and Agriculture, and Health Services. The best thing a community can do is have a plan."

An e-mail sent Sept. 11 from SpiritWild's Sandie Elliott to Buckman said, "If the lab would have listened to me on Aug. 14 when I suspected LPAI (low pathogenic avian influenza) preceding the botulism, we could have done lots of testing. I believe the topic of a program outline is soon to be a subject that the board of supervisors will be demanding." Elliott added, "It's about time."

Lake County Administrator Kelly Cox initiated a meeting to occur next week (and possibly as early as Monday) between his office, Elliott, the County Agricultural Commission, Animal Control and representatives from both the Sheriff and Health Departments to discuss these important issues.

Contact Cynthia Davis at cdavis@record-bee.com.
 
Re: Officials discuss animal die-off response

No offense to the reporter but this article is very confusing.

"There are 16 h types and 9 N types which can mix together into 144 different combinations, most of which are no problem," Retallack added, "So when you get an H5 you send it in for further testing to determine the N type. So far it looks like there is nothing to worry about."


At this point in the article they say "So far it looks like there is nothing to worry about." This is interesting because it doesn't say that they have scientific evidence yet to support such a statement.

Let's continue.

The duck is undergoing further testing at the USDA's National Veterinary Services Laboratory in Ames, Iowa, said Retallack. The lab would have notified them if the sub type was N1 or something to be concerned about, she added.​
So the lab in Ames, Iowa has not told them what the results of the tests are yet. They are just saying that the lab would have notified them if it was something to worry about.

Hmmm....


"The most important thing they were able to figure out is that it was low pathogen," said Fish and Game biologist, Patrick Foy.

What???

Wait. I thought they just said that they haven't got the test results back on the DEAD duck.

Given that the avian influenza strain identified in the local duck is low pathogen, it is not to be confused with the bird flu strain causing concern around the globe, called H5N1.

How the heck were they able to figure out that is was low path? What strain of low path?

Two paragraphs ago they were waiting on test results, and now they know it was low path....

Do you see the disconnect??
 
Re: Officials discuss animal die-off response

I remember Niman wrote about judging the pathogenics of the virus by the size of the 'insert' made in the PCR test wrt the HA cleavage site.

I'm not sure exactly which test was performed by the California Dept Fish and Game to determine the H5 subtype. but Patrick Foy, a biologist who works there says 'they' were able to figure out it was a low-path virus. Maybe they measured the clevage insert size during a PCR test? :)

With only one of the dead duck with a LP H5, this doesn't sound like the Virus we've been watching.
Not yet anyway.
 
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Re: Officials discuss animal die-off response

I spoke to Cynthia Davis, the reporter for the Record Bee who authored this article. She confirmed her story including the quotes of Alexia Retallack.

"....Alexia Retallack, a California Fish and Game information officer, said that all indications show that this is a low-pathogen strain of bird flu that is not a threat to human health.

In this particular case, we had a positive hit for avian influenza and the subtype is H5 category," said Retallack...."


I have left 2 messages for Ms. Retallack to call me back. I will call again later.
 
Re: Officials discuss animal die-off response

I spoke to the veterinarian, at the laboratory, who was in charge of the initial testing on the duck in question. She said:

1) The duck tested positive for H5.
2) The initial general PCR test was positive for influenza.
3) The second PCR that tests for H5 or H7 was positive for H5.
4) The CDC Assay PCR test for Asian (H5N1) - Highly Pathogenic Test was negative.
5) The samples were sent to the USDA's National Veterinary Services Laboratory in Ames, Iowa, recently. The results are not back yet.
 
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