tetano
Editor, Senior Moderator
J Virol. 2011 Jul 20. [Epub ahead of print]
Ocular tropism of influenza A viruses: Identification of H7 subtype-specific host responses in human respiratory and ocular cells.
Belser JA, Zeng H, Katz JM, Tumpey TM.
Source
Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333.
Abstract
Highly pathogenic avian influenza (HPAI) H7 virus infection in humans frequently results in conjunctivitis as a major symptom. However, our understanding of what properties govern virus subtype-specific tropism, and the host responses responsible for eliciting ocular inflammation and pathogenicity following influenza virus infection, are not well understood. To study virus-host interactions in ocular tissue, we infected primary human corneal and conjunctival epithelial cells with H7, H5, and H1 subtype viruses. We found that numerous virus subtypes were capable of infecting and replicating in multiple human ocular cell types, with highest titers observed with highly pathogenic H7N7 and H5N1 viruses. Similar patterns of proinflammatory cytokine and chemokine production following influenza virus infection were observed between ocular and respiratory cells. However, primary ocular cells infected with HPAI H7N7 viruses were found to induce elevated levels of IL-1β, a cytokine previously implicated in ocular disease pathology. Furthermore, H7N7 virus infection of corneal epithelial cells resulted in enhanced and significant increases in the expression of genes related to NFκB signal transduction compared with H5N1 or H1N1 virus infection. Differential induction of cytokines and signaling pathways in human ocular cells following H7 virus infection marks the first association of H7 subtype-specific host responses with ocular tropism and pathogenicity. In particular, heightened expression of genes related to NFκB-mediated signaling transduction following HPAI H7N7 virus infection in primary corneal epithelial cells, but not respiratory cells, identifies activation of a signaling pathway that correlates with the ocular tropism of influenza viruses within this subtype.
PMID:
21775456
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21775456
Ocular tropism of influenza A viruses: Identification of H7 subtype-specific host responses in human respiratory and ocular cells.
Belser JA, Zeng H, Katz JM, Tumpey TM.
Source
Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333.
Abstract
Highly pathogenic avian influenza (HPAI) H7 virus infection in humans frequently results in conjunctivitis as a major symptom. However, our understanding of what properties govern virus subtype-specific tropism, and the host responses responsible for eliciting ocular inflammation and pathogenicity following influenza virus infection, are not well understood. To study virus-host interactions in ocular tissue, we infected primary human corneal and conjunctival epithelial cells with H7, H5, and H1 subtype viruses. We found that numerous virus subtypes were capable of infecting and replicating in multiple human ocular cell types, with highest titers observed with highly pathogenic H7N7 and H5N1 viruses. Similar patterns of proinflammatory cytokine and chemokine production following influenza virus infection were observed between ocular and respiratory cells. However, primary ocular cells infected with HPAI H7N7 viruses were found to induce elevated levels of IL-1β, a cytokine previously implicated in ocular disease pathology. Furthermore, H7N7 virus infection of corneal epithelial cells resulted in enhanced and significant increases in the expression of genes related to NFκB signal transduction compared with H5N1 or H1N1 virus infection. Differential induction of cytokines and signaling pathways in human ocular cells following H7 virus infection marks the first association of H7 subtype-specific host responses with ocular tropism and pathogenicity. In particular, heightened expression of genes related to NFκB-mediated signaling transduction following HPAI H7N7 virus infection in primary corneal epithelial cells, but not respiratory cells, identifies activation of a signaling pathway that correlates with the ocular tropism of influenza viruses within this subtype.
PMID:
21775456
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21775456