tetano
Editor, Senior Moderator
Vet Microbiol. 2016 Jan 15;182:95-101. doi: 10.1016/j.vetmic.2015.10.028. Epub 2015 Oct 31.
[h=1]NS-gene based phylogenetic analysis of equine influenza viruses isolated in Poland.[/h] Kwasnik M[SUP]1[/SUP], Gora IM[SUP]2[/SUP], Rola J[SUP]2[/SUP], Zmudzinski JF[SUP]2[/SUP], Rozek W[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The phylogenetic analysis of influenza virus is based mainly on the variable hemagglutinin or neuraminidase genes. However, some discrete evolutionary trends might be revealed when more conservative genes are considered. We compared all available in GenBank database full length NS sequences of equine influenza virus including Polish isolates. Four nucleotides at positions A202, A237, T672 and A714 and three amino acids at positions H59, K71 and S216 which are also present in A/eq/Pulawy/2006 and A/eq/Pulawy/2008 may be discriminating for the Florida sublineage. Threonine at position 83 seems to be characteristic for EIV strains of Florida 2 isolated after 2007. There are nine common substitutions in the NS sequences of A/eq/Pulawy/2005, A/eq/Aboyne/1/2005 and A/eq/Lincolnshire/1/2006 in relation to the reference strain A/eq/Miami/63, resulting in four amino acid changes in NS1 protein (I56, E76, K140, E179) and one in NEP (R22). We grouped these strains as "Aboyne-like". Some of the listed changes were also observed in H7N7 strains isolated between 1956 and 1966, in A/eq/Jilin/89 or in pre-divergent H3N8 strains. Two hypotheses regarding the origin of this group were postulated: three independent transfers of avian influenza viruses into the equine population or reassortation between H7N7 and H3N8 EIV. Similarities of the NS sequences of "Aboyne like" viruses to viruses isolated in the fifties or seventies can reflect a phenomenon of "frozen evolution".
Copyright ? 2015 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] EIV; Equine influenza virus; NEP; NS1; Phylogenetic analysis
PMID: 26711034 [PubMed - in process]
[h=1]NS-gene based phylogenetic analysis of equine influenza viruses isolated in Poland.[/h] Kwasnik M[SUP]1[/SUP], Gora IM[SUP]2[/SUP], Rola J[SUP]2[/SUP], Zmudzinski JF[SUP]2[/SUP], Rozek W[SUP]2[/SUP].
[h=3]Author information[/h]
- [SUP]1[/SUP]Department of Virology, National Veterinary Research Institute, Al. Partyzantow 57, Pulawy 24-100, Poland. Electronic address: malgorzata.kwasnik@piwet.pulawy.pl.
- [SUP]2[/SUP]Department of Virology, National Veterinary Research Institute, Al. Partyzantow 57, Pulawy 24-100, Poland.
[h=3]Abstract[/h] The phylogenetic analysis of influenza virus is based mainly on the variable hemagglutinin or neuraminidase genes. However, some discrete evolutionary trends might be revealed when more conservative genes are considered. We compared all available in GenBank database full length NS sequences of equine influenza virus including Polish isolates. Four nucleotides at positions A202, A237, T672 and A714 and three amino acids at positions H59, K71 and S216 which are also present in A/eq/Pulawy/2006 and A/eq/Pulawy/2008 may be discriminating for the Florida sublineage. Threonine at position 83 seems to be characteristic for EIV strains of Florida 2 isolated after 2007. There are nine common substitutions in the NS sequences of A/eq/Pulawy/2005, A/eq/Aboyne/1/2005 and A/eq/Lincolnshire/1/2006 in relation to the reference strain A/eq/Miami/63, resulting in four amino acid changes in NS1 protein (I56, E76, K140, E179) and one in NEP (R22). We grouped these strains as "Aboyne-like". Some of the listed changes were also observed in H7N7 strains isolated between 1956 and 1966, in A/eq/Jilin/89 or in pre-divergent H3N8 strains. Two hypotheses regarding the origin of this group were postulated: three independent transfers of avian influenza viruses into the equine population or reassortation between H7N7 and H3N8 EIV. Similarities of the NS sequences of "Aboyne like" viruses to viruses isolated in the fifties or seventies can reflect a phenomenon of "frozen evolution".
Copyright ? 2015 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] EIV; Equine influenza virus; NEP; NS1; Phylogenetic analysis
PMID: 26711034 [PubMed - in process]