tetano
Editor, Senior Moderator
J Microbiol Biotechnol. 2014 Feb 3. [Epub ahead of print]
Novel phage display-derived H5N1-specific scFv's with potential use in rapid avian flu diagnosis.
Wu J, Zeng XQ, Zhang HB, Ni HZ, Pei L, Zou LR, Liang LJ, Zhang X, Lin JY, Ke CW.
Author information
Abstract
The highly-pathogenic-avian-influenza-A (HPAI) viruses of the H5N1 subtype not only infect poultry but have also been spreading to humans. Although new antiviral drugs and vaccination would be effective, rapid detection would be more efficient to control the outbreak of infections. Here a phage-display library was applied to select antibody fragments against HPAI strain A/Hubei/1/2010. Three clones were thus selected and sequenced. Hemagglutinin inhibition (HI) assay of the three scFvs indicted that none of them exhibited hemagglutination inhibition activity for the H5N1 virus, but they did show a higher binding affinity for several HPAI H5N1 compared to the other influenza viruses. Through ELISA assay HA protein were confirmed the target of the scFvs, and results of the protein structure simulation showed that all the selected scFv's bound to the HA2 subunit of the HA protein. Generally, the three scFV's might be useful for developing specific detection tool for surveillance of HPAI epidemic strain.
PMID:
24487936
[PubMed - as supplied by publisher]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/24487936
Novel phage display-derived H5N1-specific scFv's with potential use in rapid avian flu diagnosis.
Wu J, Zeng XQ, Zhang HB, Ni HZ, Pei L, Zou LR, Liang LJ, Zhang X, Lin JY, Ke CW.
Author information
Abstract
The highly-pathogenic-avian-influenza-A (HPAI) viruses of the H5N1 subtype not only infect poultry but have also been spreading to humans. Although new antiviral drugs and vaccination would be effective, rapid detection would be more efficient to control the outbreak of infections. Here a phage-display library was applied to select antibody fragments against HPAI strain A/Hubei/1/2010. Three clones were thus selected and sequenced. Hemagglutinin inhibition (HI) assay of the three scFvs indicted that none of them exhibited hemagglutination inhibition activity for the H5N1 virus, but they did show a higher binding affinity for several HPAI H5N1 compared to the other influenza viruses. Through ELISA assay HA protein were confirmed the target of the scFvs, and results of the protein structure simulation showed that all the selected scFv's bound to the HA2 subunit of the HA protein. Generally, the three scFV's might be useful for developing specific detection tool for surveillance of HPAI epidemic strain.
PMID:
24487936
[PubMed - as supplied by publisher]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/24487936