tetano
Editor, Senior Moderator
New Microbes New Infect
. 2022 Jul 6;101001.
doi: 10.1016/j.nmni.2022.101001. Online ahead of print.
First case of within-host co-infection of different SARS-CoV-2 variants in Ecuador
Juan Carlos Fernandez-Cadena[SUP] 1 [/SUP], Mateo Carvajal[SUP] 2 [/SUP], Erika Muñoz[SUP] 2 [/SUP], Belén Prado-Vivar[SUP] 2 [/SUP], Sully Marquez[SUP] 2 [/SUP], Stefanie Proaño[SUP] 2 [/SUP], Rosa Bayas[SUP] 2 [/SUP], Juan José Guadalupe[SUP] 3 [/SUP], Mónica Becerra-Wong[SUP] 2 [/SUP], Bernardo Gutierrez[SUP] 3 4 [/SUP], Gabriel Morey-Leon[SUP] 5 [/SUP], Gabriel Trueba[SUP] 2 [/SUP], Michelle Grunauer[SUP] 6 7 [/SUP], Verónica Barragán[SUP] 2 [/SUP], Patricio Rojas-Silva[SUP] 2 [/SUP], Derly Andrade-Molina[SUP] 8 [/SUP], Paúl Cárdenas[SUP] 2 [/SUP]
Affiliations
Abstract
Background: COVID-19 infection caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can cause mild symptoms to severe illness and death. Co-infections of SARS-CoV-2 with other respiratory viruses has been described. However, two SARS-CoV-2 lineage co-infection have been rarely reported.
Methodology: A genotyping analysis and two different types of whole genome sequencing were performed (Illumina MiniSeq and ONT MinION). When examining the phylogenetic analysis in NextClade and Pangolin webservers, and considering the genotyping findings, conflicting results were obtained.
Results: The raw data of the sequencing was analyzed, and nucleotide variants were identified between different reads of the virus genome. B.1 and P.1 lineages were identified within the same sample.
Conclusions: We concluded that this is a co-infection case with two SARS-CoV-2 lineages, the first one reported in Ecuador.
. 2022 Jul 6;101001.
doi: 10.1016/j.nmni.2022.101001. Online ahead of print.
First case of within-host co-infection of different SARS-CoV-2 variants in Ecuador
Juan Carlos Fernandez-Cadena[SUP] 1 [/SUP], Mateo Carvajal[SUP] 2 [/SUP], Erika Muñoz[SUP] 2 [/SUP], Belén Prado-Vivar[SUP] 2 [/SUP], Sully Marquez[SUP] 2 [/SUP], Stefanie Proaño[SUP] 2 [/SUP], Rosa Bayas[SUP] 2 [/SUP], Juan José Guadalupe[SUP] 3 [/SUP], Mónica Becerra-Wong[SUP] 2 [/SUP], Bernardo Gutierrez[SUP] 3 4 [/SUP], Gabriel Morey-Leon[SUP] 5 [/SUP], Gabriel Trueba[SUP] 2 [/SUP], Michelle Grunauer[SUP] 6 7 [/SUP], Verónica Barragán[SUP] 2 [/SUP], Patricio Rojas-Silva[SUP] 2 [/SUP], Derly Andrade-Molina[SUP] 8 [/SUP], Paúl Cárdenas[SUP] 2 [/SUP]
Affiliations
- PMID: 35818397
- PMCID: PMC9259011
- DOI: 10.1016/j.nmni.2022.101001
Abstract
Background: COVID-19 infection caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can cause mild symptoms to severe illness and death. Co-infections of SARS-CoV-2 with other respiratory viruses has been described. However, two SARS-CoV-2 lineage co-infection have been rarely reported.
Methodology: A genotyping analysis and two different types of whole genome sequencing were performed (Illumina MiniSeq and ONT MinION). When examining the phylogenetic analysis in NextClade and Pangolin webservers, and considering the genotyping findings, conflicting results were obtained.
Results: The raw data of the sequencing was analyzed, and nucleotide variants were identified between different reads of the virus genome. B.1 and P.1 lineages were identified within the same sample.
Conclusions: We concluded that this is a co-infection case with two SARS-CoV-2 lineages, the first one reported in Ecuador.