tetano
Editor, Senior Moderator
J Clin Microbiol. 2012 Oct 17. [Epub ahead of print]
Neutralizing DNA Aptamers Against Swine Influenza H3N2 Viruses.
Wongphatcharachai M, Wang P, Enomoto S, Webby RJ, Gramer MR, Amonsin A, Sreevatsan S.
Source
Emerging and Re-emerging Infectious Diseases in Animals, Research Unit, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Abstract
Triple reassortant influenza A viruses (IAVs) of swine, particularly the North American H3N2 subtype, circulate in swine-herds and may reassort and result in the emergence of novel zoonotic strains. Current diagnostic tools rely on isolation of the viruses followed by serotyping by hemagglutination or genome sequencing, both of which can be expensive and time consuming. Thus novel subtype specific ligands and methods are needed for rapid testing and subtyping of IAVs in the field. To address this need, we selected DNA aptamers against the recombinant HA protein from swine IAV H3 cluster IV using systematic evolution of ligands by exponential enrichment (SELEX). Four candidate aptamers (HA68, HA7, HA2a and HA2b) were identified and characterized. The dissociation constants (K(D)) of aptamers - HA68, HA7, HA2a and HA2b against recombinant H3 protein were 7.1 nM, 22.3 nM, 16.0 nM and 3.7 nM, respectively. The binding site of HA68 to H3 was identified to be between nucleotide residues 8 and 40. All aptamers inhibited H3 hemagglutination. HA68 was highly specific to all four lineages within the North American H3N2 subtype. Further, the other three aptamers specifically identified live viruses belonging to the phylogenetic clusters I, II/III and IV especially the virus that closely related to the recent H3N2 variant (H3N2v). Aptamer HA68 was also able to bind and detect H3N2v isolated from recent human cases. In conclusion, we provide subtype specific aptamers against H3N2 IAVs of swine that can now be used in rapid detection and typing protocols for field applications.
PMID:
23077124
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23077124
Neutralizing DNA Aptamers Against Swine Influenza H3N2 Viruses.
Wongphatcharachai M, Wang P, Enomoto S, Webby RJ, Gramer MR, Amonsin A, Sreevatsan S.
Source
Emerging and Re-emerging Infectious Diseases in Animals, Research Unit, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Abstract
Triple reassortant influenza A viruses (IAVs) of swine, particularly the North American H3N2 subtype, circulate in swine-herds and may reassort and result in the emergence of novel zoonotic strains. Current diagnostic tools rely on isolation of the viruses followed by serotyping by hemagglutination or genome sequencing, both of which can be expensive and time consuming. Thus novel subtype specific ligands and methods are needed for rapid testing and subtyping of IAVs in the field. To address this need, we selected DNA aptamers against the recombinant HA protein from swine IAV H3 cluster IV using systematic evolution of ligands by exponential enrichment (SELEX). Four candidate aptamers (HA68, HA7, HA2a and HA2b) were identified and characterized. The dissociation constants (K(D)) of aptamers - HA68, HA7, HA2a and HA2b against recombinant H3 protein were 7.1 nM, 22.3 nM, 16.0 nM and 3.7 nM, respectively. The binding site of HA68 to H3 was identified to be between nucleotide residues 8 and 40. All aptamers inhibited H3 hemagglutination. HA68 was highly specific to all four lineages within the North American H3N2 subtype. Further, the other three aptamers specifically identified live viruses belonging to the phylogenetic clusters I, II/III and IV especially the virus that closely related to the recent H3N2 variant (H3N2v). Aptamer HA68 was also able to bind and detect H3N2v isolated from recent human cases. In conclusion, we provide subtype specific aptamers against H3N2 IAVs of swine that can now be used in rapid detection and typing protocols for field applications.
PMID:
23077124
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23077124