tetano
Editor, Senior Moderator
PLoS One. 2018 Dec 31;13(12):e0210124. doi: 10.1371/journal.pone.0210124. eCollection 2018.
[h=1]Neutralization and hemagglutination-inhibition antibodies following influenza vaccination of HIV-infected and HIV-uninfected pregnant women.[/h] Nunes MC[SUP]1,[/SUP][SUP]2[/SUP], Weinberg A[SUP]3[/SUP], Cutland CL[SUP]1,[/SUP][SUP]2[/SUP], Jones S[SUP]1,[/SUP][SUP]2[/SUP], Wang D[SUP]4[/SUP], Dighero-Kemp B[SUP]4[/SUP], Levine MZ[SUP]4[/SUP], Wairagkar N[SUP]5[/SUP], Madhi SA[SUP]1,[/SUP][SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] We previously reported that despite HIV-infected pregnant women had modest humoral immune responses to inactivated influenza vaccine (IIV) measured by hemagglutination-inhibition (HAI) assay, the observed vaccine efficacy against influenza disease was higher than predicted by HAI; suggesting that IIV may confer protection to HIV-infected individuals by additional mechanisms. We evaluated the response to IIV by microneutralization (MN) and HAI assays and correlated both methods in HIV-infected and HIV-uninfected pregnant women.
[h=4]METHODS:[/h] MN and HAI antibodies were measured pre-vaccination and approximately one-month post-vaccination in 80 HIV-infected and 75 HIV-uninfected women who received IIV. Geometric mean titers (GMTs), fold-change in titers and seroconversion rates were determined for the three influenza stains in the vaccine.
[h=4]RESULTS:[/h] After vaccination there were significant increases in MN and HAI GMTs for the three vaccine strains in both HIV-infected and HIV-uninfected women. HIV-infected women had, however, a lower immune response compared to HIV-uninfected. Fold-increases were 2 to 3-times higher for MN assay compared to HAI assay for the influenza-A strains. Also a higher percentage of women seroconverted by MN than by HAI assay for the influenza-A strains. There was high positive correlation between MN and HAI assays, except for the B/Victoria strain at pre-vaccination.
[h=4]CONCLUSIONS:[/h] In general, the MN assay was more sensitive than the HAI assay. Microneutralization antibodies might correlate better with protection against influenza infection.
PMID: 30596775 DOI: 10.1371/journal.pone.0210124
[h=1]Neutralization and hemagglutination-inhibition antibodies following influenza vaccination of HIV-infected and HIV-uninfected pregnant women.[/h] Nunes MC[SUP]1,[/SUP][SUP]2[/SUP], Weinberg A[SUP]3[/SUP], Cutland CL[SUP]1,[/SUP][SUP]2[/SUP], Jones S[SUP]1,[/SUP][SUP]2[/SUP], Wang D[SUP]4[/SUP], Dighero-Kemp B[SUP]4[/SUP], Levine MZ[SUP]4[/SUP], Wairagkar N[SUP]5[/SUP], Madhi SA[SUP]1,[/SUP][SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] We previously reported that despite HIV-infected pregnant women had modest humoral immune responses to inactivated influenza vaccine (IIV) measured by hemagglutination-inhibition (HAI) assay, the observed vaccine efficacy against influenza disease was higher than predicted by HAI; suggesting that IIV may confer protection to HIV-infected individuals by additional mechanisms. We evaluated the response to IIV by microneutralization (MN) and HAI assays and correlated both methods in HIV-infected and HIV-uninfected pregnant women.
[h=4]METHODS:[/h] MN and HAI antibodies were measured pre-vaccination and approximately one-month post-vaccination in 80 HIV-infected and 75 HIV-uninfected women who received IIV. Geometric mean titers (GMTs), fold-change in titers and seroconversion rates were determined for the three influenza stains in the vaccine.
[h=4]RESULTS:[/h] After vaccination there were significant increases in MN and HAI GMTs for the three vaccine strains in both HIV-infected and HIV-uninfected women. HIV-infected women had, however, a lower immune response compared to HIV-uninfected. Fold-increases were 2 to 3-times higher for MN assay compared to HAI assay for the influenza-A strains. Also a higher percentage of women seroconverted by MN than by HAI assay for the influenza-A strains. There was high positive correlation between MN and HAI assays, except for the B/Victoria strain at pre-vaccination.
[h=4]CONCLUSIONS:[/h] In general, the MN assay was more sensitive than the HAI assay. Microneutralization antibodies might correlate better with protection against influenza infection.
PMID: 30596775 DOI: 10.1371/journal.pone.0210124