tetano
Editor, Senior Moderator
Mycobiology. 2014 Jun;42(2):189-92. doi: 10.5941/MYCO.2014.42.2.189. Epub 2014 Jun 30.
Neuraminidase Inhibitors from the Fermentation Broth of Phellinus linteus.
Hwang BS1, Lee MS1, Lee SW1, Lee IK1, Seo GS2, Choi HJ3, Yun BS1.
Author information
Abstract
During a search for neuraminidase inhibitors derived from medicinal fungi, we found that the fermentation broth of Phellinus linteus exhibited potent neuraminidase inhibitory activity. Through bioassay-guided fractionation, two active compounds were purified from the ethyl acetate-soluble portion of the fermentation broth of P. linteus. These structures were identified as inotilone (1) and 4-(3,4-dihydroxyphenyl)-3-buten-2-one (2) by spectroscopic methods. Compounds 1 and 2 inhibited H1N1 neuraminidase activity with IC50 values of 29.1 and 125.6 ?M, respectively, in a dose-dependent manner. They also exhibited an antiviral effect in a viral cytopathic effect reduction assay using MDCK cells. These results suggest that compounds 1 and 2 from the culture broth of P. linteus would be good candidates for the prevention and therapeutic strategies towards viral infections.
KEYWORDS:
4-(3,4-Dihydroxyphenyl)-3-buten-2-one; Anti-influenza agent; Inotilone; Neuraminidase inhibitor; Phellinus linteus
PMID:
25071390
[PubMed]
PMCID:
PMC4112237
Free PMC Article
http://www.ncbi.nlm.nih.gov/pubmed/25071390
Neuraminidase Inhibitors from the Fermentation Broth of Phellinus linteus.
Hwang BS1, Lee MS1, Lee SW1, Lee IK1, Seo GS2, Choi HJ3, Yun BS1.
Author information
Abstract
During a search for neuraminidase inhibitors derived from medicinal fungi, we found that the fermentation broth of Phellinus linteus exhibited potent neuraminidase inhibitory activity. Through bioassay-guided fractionation, two active compounds were purified from the ethyl acetate-soluble portion of the fermentation broth of P. linteus. These structures were identified as inotilone (1) and 4-(3,4-dihydroxyphenyl)-3-buten-2-one (2) by spectroscopic methods. Compounds 1 and 2 inhibited H1N1 neuraminidase activity with IC50 values of 29.1 and 125.6 ?M, respectively, in a dose-dependent manner. They also exhibited an antiviral effect in a viral cytopathic effect reduction assay using MDCK cells. These results suggest that compounds 1 and 2 from the culture broth of P. linteus would be good candidates for the prevention and therapeutic strategies towards viral infections.
KEYWORDS:
4-(3,4-Dihydroxyphenyl)-3-buten-2-one; Anti-influenza agent; Inotilone; Neuraminidase inhibitor; Phellinus linteus
PMID:
25071390
[PubMed]
PMCID:
PMC4112237
Free PMC Article
http://www.ncbi.nlm.nih.gov/pubmed/25071390