tetano
Editor, Senior Moderator
Nature
. 2020 Dec 4.
doi: 10.1038/s41586-020-03041-6. Online ahead of print.
Correlates of protection against SARS-CoV-2 in rhesus macaques
Katherine McMahan[SUP] 1 [/SUP], Jingyou Yu[SUP] 1 [/SUP], Noe B Mercado[SUP] 1 [/SUP], Carolin Loos[SUP] 2 3 [/SUP], Lisa H Tostanoski[SUP] 1 [/SUP], Abishek Chandrashekar[SUP] 1 [/SUP], Jinyan Liu[SUP] 1 [/SUP], Lauren Peter[SUP] 1 [/SUP], Caroline Atyeo[SUP] 2 4 [/SUP], Alex Zhu[SUP] 2 [/SUP], Esther A Bondzie[SUP] 1 [/SUP], Gabriel Dagotto[SUP] 1 4 [/SUP], Makda S Gebre[SUP] 1 4 [/SUP], Catherine Jacob-Dolan[SUP] 1 4 [/SUP], Zhenfeng Li[SUP] 1 [/SUP], Felix Nampanya[SUP] 1 [/SUP], Shivani Patel[SUP] 1 [/SUP], Laurent Pessaint[SUP] 5 [/SUP], Alex Van Ry[SUP] 5 [/SUP], Kelvin Blade[SUP] 5 [/SUP], Jake Yalley-Ogunro[SUP] 5 [/SUP], Mehtap Cabus[SUP] 5 [/SUP], Renita Brown[SUP] 5 [/SUP], Anthony Cook[SUP] 5 [/SUP], Elyse Teow[SUP] 5 [/SUP], Hanne Andersen[SUP] 5 [/SUP], Mark G Lewis[SUP] 5 [/SUP], Douglas A Lauffenburger[SUP] 3 [/SUP], Galit Alter[SUP] 2 6 [/SUP], Dan H Barouch[SUP] 7 8 9 10 [/SUP]
Affiliations
Abstract
Recent studies have reported protective efficacy of both natural immunity[SUP]1[/SUP] and vaccine-induced immunity[SUP]2-7[/SUP] against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) challenge in rhesus macaques. However, the importance of humoral and cellular immunity for protection against SARS-CoV-2 infection remains to be determined. Here we show that adoptive transfer of purified IgG from convalescent macaques protects na?ve recipient rhesus macaques against SARS-CoV-2 challenge in a dose dependent fashion. Depletion of CD8+ T cells in convalescent animals partially abrogated the protective efficacy of natural immunity against SARS-CoV-2 re-challenge, suggesting the importance of cellular immunity in the context of waning or subprotective antibody titers. These data demonstrate that relatively low antibody titers are sufficient for protection against SARS-CoV-2 in rhesus macaques, and that cellular immune responses may also contribute to protection if antibody responses are suboptimal. We also show that higher antibody titers are required for therapy of SARS-CoV-2 infection in macaques. These findings have important implications for the development of SARS-CoV-2 vaccines and immune-based therapeutics.
. 2020 Dec 4.
doi: 10.1038/s41586-020-03041-6. Online ahead of print.
Correlates of protection against SARS-CoV-2 in rhesus macaques
Katherine McMahan[SUP] 1 [/SUP], Jingyou Yu[SUP] 1 [/SUP], Noe B Mercado[SUP] 1 [/SUP], Carolin Loos[SUP] 2 3 [/SUP], Lisa H Tostanoski[SUP] 1 [/SUP], Abishek Chandrashekar[SUP] 1 [/SUP], Jinyan Liu[SUP] 1 [/SUP], Lauren Peter[SUP] 1 [/SUP], Caroline Atyeo[SUP] 2 4 [/SUP], Alex Zhu[SUP] 2 [/SUP], Esther A Bondzie[SUP] 1 [/SUP], Gabriel Dagotto[SUP] 1 4 [/SUP], Makda S Gebre[SUP] 1 4 [/SUP], Catherine Jacob-Dolan[SUP] 1 4 [/SUP], Zhenfeng Li[SUP] 1 [/SUP], Felix Nampanya[SUP] 1 [/SUP], Shivani Patel[SUP] 1 [/SUP], Laurent Pessaint[SUP] 5 [/SUP], Alex Van Ry[SUP] 5 [/SUP], Kelvin Blade[SUP] 5 [/SUP], Jake Yalley-Ogunro[SUP] 5 [/SUP], Mehtap Cabus[SUP] 5 [/SUP], Renita Brown[SUP] 5 [/SUP], Anthony Cook[SUP] 5 [/SUP], Elyse Teow[SUP] 5 [/SUP], Hanne Andersen[SUP] 5 [/SUP], Mark G Lewis[SUP] 5 [/SUP], Douglas A Lauffenburger[SUP] 3 [/SUP], Galit Alter[SUP] 2 6 [/SUP], Dan H Barouch[SUP] 7 8 9 10 [/SUP]
Affiliations
- PMID: 33276369
- DOI: 10.1038/s41586-020-03041-6
Abstract
Recent studies have reported protective efficacy of both natural immunity[SUP]1[/SUP] and vaccine-induced immunity[SUP]2-7[/SUP] against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) challenge in rhesus macaques. However, the importance of humoral and cellular immunity for protection against SARS-CoV-2 infection remains to be determined. Here we show that adoptive transfer of purified IgG from convalescent macaques protects na?ve recipient rhesus macaques against SARS-CoV-2 challenge in a dose dependent fashion. Depletion of CD8+ T cells in convalescent animals partially abrogated the protective efficacy of natural immunity against SARS-CoV-2 re-challenge, suggesting the importance of cellular immunity in the context of waning or subprotective antibody titers. These data demonstrate that relatively low antibody titers are sufficient for protection against SARS-CoV-2 in rhesus macaques, and that cellular immune responses may also contribute to protection if antibody responses are suboptimal. We also show that higher antibody titers are required for therapy of SARS-CoV-2 infection in macaques. These findings have important implications for the development of SARS-CoV-2 vaccines and immune-based therapeutics.