tetano
Editor, Senior Moderator
Nat Immunol
. 2020 Aug 12.
doi: 10.1038/s41590-020-0762-x. Online ahead of print.
Single-cell landscape of immunological responses in patients with COVID-19
Ji-Yuan Zhang[SUP] 1 [/SUP], Xiang-Ming Wang[SUP] 2 [/SUP], Xudong Xing[SUP] 3 [/SUP], Zhe Xu[SUP] 1 [/SUP], Chao Zhang[SUP] 1 [/SUP], Jin-Wen Song[SUP] 1 [/SUP], Xing Fan[SUP] 1 [/SUP], Peng Xia[SUP] 1 [/SUP], Jun-Liang Fu[SUP] 1 [/SUP], Si-Yu Wang[SUP] 1 [/SUP], Ruo-Nan Xu[SUP] 1 [/SUP], Xiao-Peng Dai[SUP] 1 [/SUP], Lei Shi[SUP] 1 [/SUP], Lei Huang[SUP] 1 [/SUP], Tian-Jun Jiang[SUP] 1 [/SUP], Ming Shi[SUP] 1 [/SUP], Yuxia Zhang[SUP] 4 [/SUP], Alimuddin Zumla[SUP] 5 6 [/SUP], Markus Maeurer[SUP] 7 8 [/SUP], Fan Bai[SUP] 9 [/SUP], Fu-Sheng Wang[SUP] 10 [/SUP]
Affiliations
Abstract
In coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, the relationship between disease severity and the host immune response is not fully understood. Here we performed single-cell RNA sequencing in peripheral blood samples of 5 healthy donors and 13 patients with COVID-19, including moderate, severe and convalescent cases. Through determining the transcriptional profiles of immune cells, coupled with assembled T cell receptor and B cell receptor sequences, we analyzed the functional properties of immune cells. Most cell types in patients with COVID-19 showed a strong interferon-α response and an overall acute inflammatory response. Moreover, intensive expansion of highly cytotoxic effector T cell subsets, such as CD4[SUP]+[/SUP] effector-GNLY (granulysin), CD8[SUP]+[/SUP] effector-GNLY and NKT CD160, was associated with convalescence in moderate patients. In severe patients, the immune landscape featured a deranged interferon response, profound immune exhaustion with skewed T cell receptor repertoire and broad T cell expansion. These findings illustrate the dynamic nature of immune responses during disease progression.
. 2020 Aug 12.
doi: 10.1038/s41590-020-0762-x. Online ahead of print.
Single-cell landscape of immunological responses in patients with COVID-19
Ji-Yuan Zhang[SUP] 1 [/SUP], Xiang-Ming Wang[SUP] 2 [/SUP], Xudong Xing[SUP] 3 [/SUP], Zhe Xu[SUP] 1 [/SUP], Chao Zhang[SUP] 1 [/SUP], Jin-Wen Song[SUP] 1 [/SUP], Xing Fan[SUP] 1 [/SUP], Peng Xia[SUP] 1 [/SUP], Jun-Liang Fu[SUP] 1 [/SUP], Si-Yu Wang[SUP] 1 [/SUP], Ruo-Nan Xu[SUP] 1 [/SUP], Xiao-Peng Dai[SUP] 1 [/SUP], Lei Shi[SUP] 1 [/SUP], Lei Huang[SUP] 1 [/SUP], Tian-Jun Jiang[SUP] 1 [/SUP], Ming Shi[SUP] 1 [/SUP], Yuxia Zhang[SUP] 4 [/SUP], Alimuddin Zumla[SUP] 5 6 [/SUP], Markus Maeurer[SUP] 7 8 [/SUP], Fan Bai[SUP] 9 [/SUP], Fu-Sheng Wang[SUP] 10 [/SUP]
Affiliations
- PMID: 32788748
- DOI: 10.1038/s41590-020-0762-x
Abstract
In coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, the relationship between disease severity and the host immune response is not fully understood. Here we performed single-cell RNA sequencing in peripheral blood samples of 5 healthy donors and 13 patients with COVID-19, including moderate, severe and convalescent cases. Through determining the transcriptional profiles of immune cells, coupled with assembled T cell receptor and B cell receptor sequences, we analyzed the functional properties of immune cells. Most cell types in patients with COVID-19 showed a strong interferon-α response and an overall acute inflammatory response. Moreover, intensive expansion of highly cytotoxic effector T cell subsets, such as CD4[SUP]+[/SUP] effector-GNLY (granulysin), CD8[SUP]+[/SUP] effector-GNLY and NKT CD160, was associated with convalescence in moderate patients. In severe patients, the immune landscape featured a deranged interferon response, profound immune exhaustion with skewed T cell receptor repertoire and broad T cell expansion. These findings illustrate the dynamic nature of immune responses during disease progression.