tetano
Editor, Senior Moderator
Nat Immunol
. 2020 Nov 18.
doi: 10.1038/s41590-020-00832-x. Online ahead of print.
Macrophage expression and prognostic significance of the long pentraxin PTX3 in COVID-19
Enrico Brunetta[SUP] 1 2 [/SUP], Marco Folci[SUP] 1 2 [/SUP], Barbara Bottazzi[SUP] 1 [/SUP], Maria De Santis[SUP] 1 [/SUP], Giuseppe Gritti[SUP] 3 [/SUP], Alessandro Protti[SUP] 1 2 [/SUP], Sarah N Mapelli[SUP] 1 [/SUP], Stefanos Bonovas[SUP] 1 2 [/SUP], Daniele Piovani[SUP] 1 2 [/SUP], Roberto Leone[SUP] 1 [/SUP], Ilaria My[SUP] 1 2 [/SUP], Veronica Zanon[SUP] 1 [/SUP], Gianmarco Spata[SUP] 1 [/SUP], Monica Bacci[SUP] 1 [/SUP], Domenico Supino[SUP] 2 [/SUP], Silvia Carnevale[SUP] 2 [/SUP], Marina Sironi[SUP] 1 [/SUP], Sadaf Davoudian[SUP] 1 [/SUP], Clelia Peano[SUP] 1 4 [/SUP], Francesco Landi[SUP] 3 [/SUP], Fabiano Di Marco[SUP] 5 6 [/SUP], Federico Raimondi[SUP] 5 [/SUP], Andrea Gianatti[SUP] 7 [/SUP], Claudio Angelini[SUP] 1 [/SUP], Alessandro Rambaldi[SUP] 8 9 [/SUP], Cecilia Garlanda[SUP] 10 11 [/SUP], Michele Ciccarelli[SUP] 12 [/SUP], Maurizio Cecconi[SUP] 13 14 [/SUP], Alberto Mantovani[SUP] 15 16 17 [/SUP]
Affiliations
Abstract
Long pentraxin 3 (PTX3) is an essential component of humoral innate immunity, involved in resistance to selected pathogens and in the regulation of inflammation[SUP]1-3[/SUP]. The present study was designed to assess the presence and significance of PTX3 in Coronavirus Disease 2019 (COVID-19)[SUP]4-7[/SUP]. RNA-sequencing analysis of peripheral blood mononuclear cells, single-cell bioinformatics analysis and immunohistochemistry of lung autopsy samples revealed that myelomonocytic cells and endothelial cells express high levels of PTX3 in patients with COVID-19. Increased plasma concentrations of PTX3 were detected in 96 patients with COVID-19. PTX3 emerged as a strong independent predictor of 28-d mortality in multivariable analysis, better than conventional markers of inflammation, in hospitalized patients with COVID-19. The prognostic significance of PTX3 abundance for mortality was confirmed in a second independent cohort (54 patients). Thus, circulating and lung myelomonocytic cells and endothelial cells are a major source of PTX3, and PTX3 plasma concentration can serve as an independent strong prognostic indicator of short-term mortality in COVID-19.
. 2020 Nov 18.
doi: 10.1038/s41590-020-00832-x. Online ahead of print.
Macrophage expression and prognostic significance of the long pentraxin PTX3 in COVID-19
Enrico Brunetta[SUP] 1 2 [/SUP], Marco Folci[SUP] 1 2 [/SUP], Barbara Bottazzi[SUP] 1 [/SUP], Maria De Santis[SUP] 1 [/SUP], Giuseppe Gritti[SUP] 3 [/SUP], Alessandro Protti[SUP] 1 2 [/SUP], Sarah N Mapelli[SUP] 1 [/SUP], Stefanos Bonovas[SUP] 1 2 [/SUP], Daniele Piovani[SUP] 1 2 [/SUP], Roberto Leone[SUP] 1 [/SUP], Ilaria My[SUP] 1 2 [/SUP], Veronica Zanon[SUP] 1 [/SUP], Gianmarco Spata[SUP] 1 [/SUP], Monica Bacci[SUP] 1 [/SUP], Domenico Supino[SUP] 2 [/SUP], Silvia Carnevale[SUP] 2 [/SUP], Marina Sironi[SUP] 1 [/SUP], Sadaf Davoudian[SUP] 1 [/SUP], Clelia Peano[SUP] 1 4 [/SUP], Francesco Landi[SUP] 3 [/SUP], Fabiano Di Marco[SUP] 5 6 [/SUP], Federico Raimondi[SUP] 5 [/SUP], Andrea Gianatti[SUP] 7 [/SUP], Claudio Angelini[SUP] 1 [/SUP], Alessandro Rambaldi[SUP] 8 9 [/SUP], Cecilia Garlanda[SUP] 10 11 [/SUP], Michele Ciccarelli[SUP] 12 [/SUP], Maurizio Cecconi[SUP] 13 14 [/SUP], Alberto Mantovani[SUP] 15 16 17 [/SUP]
Affiliations
- PMID: 33208929
- DOI: 10.1038/s41590-020-00832-x
Abstract
Long pentraxin 3 (PTX3) is an essential component of humoral innate immunity, involved in resistance to selected pathogens and in the regulation of inflammation[SUP]1-3[/SUP]. The present study was designed to assess the presence and significance of PTX3 in Coronavirus Disease 2019 (COVID-19)[SUP]4-7[/SUP]. RNA-sequencing analysis of peripheral blood mononuclear cells, single-cell bioinformatics analysis and immunohistochemistry of lung autopsy samples revealed that myelomonocytic cells and endothelial cells express high levels of PTX3 in patients with COVID-19. Increased plasma concentrations of PTX3 were detected in 96 patients with COVID-19. PTX3 emerged as a strong independent predictor of 28-d mortality in multivariable analysis, better than conventional markers of inflammation, in hospitalized patients with COVID-19. The prognostic significance of PTX3 abundance for mortality was confirmed in a second independent cohort (54 patients). Thus, circulating and lung myelomonocytic cells and endothelial cells are a major source of PTX3, and PTX3 plasma concentration can serve as an independent strong prognostic indicator of short-term mortality in COVID-19.