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Nat Commun . Wastewater surveillance of SARS-CoV-2 from aircraft to citywide monitoring

tetano

Editor, Senior Moderator
Nat Commun


. 2025 Jun 2;16(1):5125.
doi: 10.1038/s41467-025-60490-1. Wastewater surveillance of SARS-CoV-2 from aircraft to citywide monitoring

Mariel Perez-Zabaleta[SUP] 1 2 [/SUP], Carlo Berg[SUP] 3 [/SUP], Neus Latorre-Margalef[SUP] 4 [/SUP], Isaac Owusu-Agyeman[SUP] 1 [/SUP], Ayda Kiyar[SUP] 1 [/SUP], Helene Botnen[SUP] 4 [/SUP], Caroline Schönning[SUP] 4 [/SUP], Luisa W Hugerth[SUP] 5 [/SUP], Zeynep Cetecioglu[SUP] 6 [/SUP]



Affiliations
Abstract

Wastewater monitoring is highly efficient in SARS-CoV-2 surveillance for tracking virus spread through travel, surpassing traditional airport passenger testing. This study explored the links between SARS-CoV-2 contents and variants from aircraft to city, assessing the impact of detected variants from international travellers versus the local population. A total of 969 variants using next-generation sequencing (NGS) were examined to understand the links between-aircraft, Arlanda airport, wastewater treatment plants (WWTPs), and Stockholm city-and compared these to variants detected in Stockholm hospitals from January to May 2023. SARS-CoV-2 contents in WWTPs reflected local infection rates, requiring analysis from multiple plants for an accurate city-wide infection assessment. Variants initially detected in aircraft arriving from China did not spread widely during the study period. RT-qPCR is adequate for the detection of specific variants in wastewater, including Variants Under Monitoring. However, NGS remains a powerful method for identifying novel variants. Wastewater monitoring was more effective than clinical testing in the early detection of specific variants, with notable delays observed in clinical surveillance. Furthermore, a broad range of variants are detected in wastewater that surpasses clinical tests. This underscores the vital role of wastewater-based epidemiology in managing future outbreaks and enhancing global health security.


 
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