tetano
Editor, Senior Moderator
Nat Commun
. 2024 Sep 27;15(1):8386.
doi: 10.1038/s41467-024-52427-x. Towards geospatially-resolved public-health surveillance via wastewater sequencing
Braden T Tierney[SUP] 1 2 [/SUP], Jonathan Foox[SUP] 3 [/SUP], Krista A Ryon[SUP] 3 [/SUP], Daniel Butler[SUP] 3 [/SUP], Namita Damle[SUP] 3 [/SUP], Benjamin G Young[SUP] 3 [/SUP], Christopher Mozsary[SUP] 3 [/SUP], Kristina M Babler[SUP] 4 5 [/SUP], Xue Yin[SUP] 5 [/SUP], Yamina Carattini[SUP] 6 [/SUP], David Andrews[SUP] 6 [/SUP], Alexander G Lucaci[SUP] 3 7 [/SUP], Natasha Schaefer Solle[SUP] 8 [/SUP], Naresh Kumar[SUP] 9 [/SUP], Bhavarth Shukla[SUP] 8 [/SUP], Dušica Vidović[SUP] 10 11 [/SUP], Benjamin Currall[SUP] 11 [/SUP], Sion L Williams[SUP] 11 [/SUP], Stephan C Schürer[SUP] 10 11 12 [/SUP], Mario Stevenson[SUP] 8 [/SUP], Ayaaz Amirali[SUP] 5 [/SUP], Cynthia Campos Beaver[SUP] 8 11 [/SUP], Erin Kobetz[SUP] 8 11 [/SUP], Melinda M Boone[SUP] 11 [/SUP], Brian Reding[SUP] 13 [/SUP], Jennifer Laine[SUP] 13 [/SUP], Samuel Comerford[SUP] 13 [/SUP], Walter E Lamar[SUP] 14 [/SUP], John J Tallon Jr[SUP] 15 [/SUP], Jeremy Wain Hirschberg[SUP] 3 [/SUP], Jacqueline Proszynski[SUP] 3 [/SUP], Gabriel Al Ghalith[SUP] 16 [/SUP], Kübra Can Kurt[SUP] 3 [/SUP], Mark E Sharkey[SUP] 6 [/SUP], George M Church[SUP] 17 [/SUP], George S Grills[SUP] 11 [/SUP], Helena M Solo-Gabriele[SUP] 18 [/SUP], Christopher E Mason[SUP] 19 20 21 [/SUP]
Affiliations
Wastewater is a geospatially- and temporally-linked microbial fingerprint of a given population, making it a potentially valuable tool for tracking public health across locales and time. Here, we integrate targeted and bulk RNA sequencing (N = 2238 samples) to track the viral, bacterial, and functional content over geospatially distinct areas within Miami Dade County, USA, from 2020-2022. We used targeted amplicon sequencing to track diverse SARS-CoV-2 variants across space and time, and we found a tight correspondence with positive PCR tests from University students and Miami-Dade hospital patients. Additionally, in bulk metatranscriptomic data, we demonstrate that the bacterial content of different wastewater sampling locations serving small population sizes can be used to detect putative, host-derived microorganisms that themselves have known associations with human health and diet. We also detect multiple enteric pathogens (e.g., Norovirus) and characterize viral diversity across sites. Moreover, we observed an enrichment of antimicrobial resistance genes (ARGs) in hospital wastewater; antibiotic-specific ARGs correlated to total prescriptions of those same antibiotics (e.g Ampicillin, Gentamicin). Overall, this effort lays the groundwork for systematic characterization of wastewater that can potentially influence public health decision-making.
. 2024 Sep 27;15(1):8386.
doi: 10.1038/s41467-024-52427-x. Towards geospatially-resolved public-health surveillance via wastewater sequencing
Braden T Tierney[SUP] 1 2 [/SUP], Jonathan Foox[SUP] 3 [/SUP], Krista A Ryon[SUP] 3 [/SUP], Daniel Butler[SUP] 3 [/SUP], Namita Damle[SUP] 3 [/SUP], Benjamin G Young[SUP] 3 [/SUP], Christopher Mozsary[SUP] 3 [/SUP], Kristina M Babler[SUP] 4 5 [/SUP], Xue Yin[SUP] 5 [/SUP], Yamina Carattini[SUP] 6 [/SUP], David Andrews[SUP] 6 [/SUP], Alexander G Lucaci[SUP] 3 7 [/SUP], Natasha Schaefer Solle[SUP] 8 [/SUP], Naresh Kumar[SUP] 9 [/SUP], Bhavarth Shukla[SUP] 8 [/SUP], Dušica Vidović[SUP] 10 11 [/SUP], Benjamin Currall[SUP] 11 [/SUP], Sion L Williams[SUP] 11 [/SUP], Stephan C Schürer[SUP] 10 11 12 [/SUP], Mario Stevenson[SUP] 8 [/SUP], Ayaaz Amirali[SUP] 5 [/SUP], Cynthia Campos Beaver[SUP] 8 11 [/SUP], Erin Kobetz[SUP] 8 11 [/SUP], Melinda M Boone[SUP] 11 [/SUP], Brian Reding[SUP] 13 [/SUP], Jennifer Laine[SUP] 13 [/SUP], Samuel Comerford[SUP] 13 [/SUP], Walter E Lamar[SUP] 14 [/SUP], John J Tallon Jr[SUP] 15 [/SUP], Jeremy Wain Hirschberg[SUP] 3 [/SUP], Jacqueline Proszynski[SUP] 3 [/SUP], Gabriel Al Ghalith[SUP] 16 [/SUP], Kübra Can Kurt[SUP] 3 [/SUP], Mark E Sharkey[SUP] 6 [/SUP], George M Church[SUP] 17 [/SUP], George S Grills[SUP] 11 [/SUP], Helena M Solo-Gabriele[SUP] 18 [/SUP], Christopher E Mason[SUP] 19 20 21 [/SUP]
Affiliations
- PMID: 39333485
- PMCID: PMC11436780
- DOI: 10.1038/s41467-024-52427-x
Wastewater is a geospatially- and temporally-linked microbial fingerprint of a given population, making it a potentially valuable tool for tracking public health across locales and time. Here, we integrate targeted and bulk RNA sequencing (N = 2238 samples) to track the viral, bacterial, and functional content over geospatially distinct areas within Miami Dade County, USA, from 2020-2022. We used targeted amplicon sequencing to track diverse SARS-CoV-2 variants across space and time, and we found a tight correspondence with positive PCR tests from University students and Miami-Dade hospital patients. Additionally, in bulk metatranscriptomic data, we demonstrate that the bacterial content of different wastewater sampling locations serving small population sizes can be used to detect putative, host-derived microorganisms that themselves have known associations with human health and diet. We also detect multiple enteric pathogens (e.g., Norovirus) and characterize viral diversity across sites. Moreover, we observed an enrichment of antimicrobial resistance genes (ARGs) in hospital wastewater; antibiotic-specific ARGs correlated to total prescriptions of those same antibiotics (e.g Ampicillin, Gentamicin). Overall, this effort lays the groundwork for systematic characterization of wastewater that can potentially influence public health decision-making.