tetano
Editor, Senior Moderator
Nat Commun
. 2021 Feb 5;12(1):802.
doi: 10.1038/s41467-021-21121-7.
Rapid electrochemical detection of coronavirus SARS-CoV-2
Thanyarat Chaibun[SUP] 1 [/SUP], Jiratchaya Puenpa[SUP] 2 [/SUP], Tatchanun Ngamdee[SUP] 3 [/SUP], Nimaradee Boonapatcharoen[SUP] 4 [/SUP], Pornpat Athamanolap[SUP] 1 [/SUP], Anthony Peter O'Mullane[SUP] 5 [/SUP], Sompong Vongpunsawad[SUP] 2 [/SUP], Yong Poovorawan[SUP] 2 [/SUP], Su Yin Lee[SUP] 6 7 [/SUP], Benchaporn Lertanantawong[SUP] 8 [/SUP]
Affiliations
Abstract
Coronavirus disease 2019 (COVID-19) is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Diagnosis of COVID-19 depends on quantitative reverse transcription PCR (qRT-PCR), which is time-consuming and requires expensive instrumentation. Here, we report an ultrasensitive electrochemical biosensor based on isothermal rolling circle amplification (RCA) for rapid detection of SARS-CoV-2. The assay involves the hybridization of the RCA amplicons with probes that were functionalized with redox active labels that are detectable by an electrochemical biosensor. The one-step sandwich hybridization assay could detect as low as 1 copy/μL of N and S genes, in less than 2 h. Sensor evaluation with 106 clinical samples, including 41 SARS-CoV-2 positive and 9 samples positive for other respiratory viruses, gave a 100% concordance result with qRT-PCR, with complete correlation between the biosensor current signals and quantitation cycle (Cq) values. In summary, this biosensor could be used as an on-site, real-time diagnostic test for COVID-19.
. 2021 Feb 5;12(1):802.
doi: 10.1038/s41467-021-21121-7.
Rapid electrochemical detection of coronavirus SARS-CoV-2
Thanyarat Chaibun[SUP] 1 [/SUP], Jiratchaya Puenpa[SUP] 2 [/SUP], Tatchanun Ngamdee[SUP] 3 [/SUP], Nimaradee Boonapatcharoen[SUP] 4 [/SUP], Pornpat Athamanolap[SUP] 1 [/SUP], Anthony Peter O'Mullane[SUP] 5 [/SUP], Sompong Vongpunsawad[SUP] 2 [/SUP], Yong Poovorawan[SUP] 2 [/SUP], Su Yin Lee[SUP] 6 7 [/SUP], Benchaporn Lertanantawong[SUP] 8 [/SUP]
Affiliations
- PMID: 33547323
- DOI: 10.1038/s41467-021-21121-7
Abstract
Coronavirus disease 2019 (COVID-19) is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Diagnosis of COVID-19 depends on quantitative reverse transcription PCR (qRT-PCR), which is time-consuming and requires expensive instrumentation. Here, we report an ultrasensitive electrochemical biosensor based on isothermal rolling circle amplification (RCA) for rapid detection of SARS-CoV-2. The assay involves the hybridization of the RCA amplicons with probes that were functionalized with redox active labels that are detectable by an electrochemical biosensor. The one-step sandwich hybridization assay could detect as low as 1 copy/μL of N and S genes, in less than 2 h. Sensor evaluation with 106 clinical samples, including 41 SARS-CoV-2 positive and 9 samples positive for other respiratory viruses, gave a 100% concordance result with qRT-PCR, with complete correlation between the biosensor current signals and quantitation cycle (Cq) values. In summary, this biosensor could be used as an on-site, real-time diagnostic test for COVID-19.