tetano
Editor, Senior Moderator
Nat Commun
. 2021 Apr 9;12(1):2133.
doi: 10.1038/s41467-021-22449-w.
IL-33 expression in response to SARS-CoV-2 correlates with seropositivity in COVID-19 convalescent individuals
Michal A Stanczak[SUP] #[/SUP][SUP] 1 [/SUP], David E Sanin[SUP] #[/SUP][SUP] 1 [/SUP], Petya Apostolova[SUP] #[/SUP][SUP] 1 [/SUP], Gabriele Nerz[SUP] 2 [/SUP], Dimitrios Lampaki[SUP] 2 [/SUP], Maike Hofmann[SUP] 3 [/SUP], Daniel Steinmann[SUP] 4 [/SUP], Marvin Krohn-Grimberghe[SUP] 5 [/SUP], Robert Thimme[SUP] 3 [/SUP], Gerhard Mittler[SUP] 2 [/SUP], Cornelius F Waller[SUP] #[/SUP][SUP] 6 [/SUP], Edward J Pearce[SUP] #[/SUP][SUP] 7 8 9 [/SUP], Erika L Pearce[SUP] #[/SUP][SUP] 10 11 [/SUP]
Affiliations
Abstract
Our understanding of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still developing. We perform an observational study to investigate seroprevalence and immune responses in subjects professionally exposed to SARS-CoV-2 and their family members (155 individuals; ages 5-79 years). Seropositivity for SARS-CoV-2 Spike glycoprotein aligns with PCR results that confirm the previous infection. Anti-Spike IgG/IgM titers remain high 60 days post-infection and do not strongly associate with symptoms, except for fever. We analyze PBMCs from a subset of seropositive and seronegative adults. TLR7 agonist-activation reveals an increased population of IL-6[SUP]+[/SUP]TNF[SUP]-[/SUP]IL-1?[SUP]+[/SUP] monocytes, while SARS-CoV-2 peptide stimulation elicits IL-33, IL-6, IFNa2, and IL-23 expression in seropositive individuals. IL-33 correlates with CD4[SUP]+[/SUP] T cell activation in PBMCs from convalescent subjects and is likely due to T cell-mediated effects on IL-33-producing cells. IL-33 is associated with pulmonary infection and chronic diseases like asthma and COPD, but its role in COVID-19 is unknown. Analysis of published scRNAseq data of bronchoalveolar lavage fluid (BALF) from patients with mild to severe COVID-19 reveals a population of IL-33-producing cells that increases with the disease. Together these findings show that IL-33 production is linked to SARS-CoV-2 infection and warrant further investigation of IL-33 in COVID-19 pathogenesis and immunity.
. 2021 Apr 9;12(1):2133.
doi: 10.1038/s41467-021-22449-w.
IL-33 expression in response to SARS-CoV-2 correlates with seropositivity in COVID-19 convalescent individuals
Michal A Stanczak[SUP] #[/SUP][SUP] 1 [/SUP], David E Sanin[SUP] #[/SUP][SUP] 1 [/SUP], Petya Apostolova[SUP] #[/SUP][SUP] 1 [/SUP], Gabriele Nerz[SUP] 2 [/SUP], Dimitrios Lampaki[SUP] 2 [/SUP], Maike Hofmann[SUP] 3 [/SUP], Daniel Steinmann[SUP] 4 [/SUP], Marvin Krohn-Grimberghe[SUP] 5 [/SUP], Robert Thimme[SUP] 3 [/SUP], Gerhard Mittler[SUP] 2 [/SUP], Cornelius F Waller[SUP] #[/SUP][SUP] 6 [/SUP], Edward J Pearce[SUP] #[/SUP][SUP] 7 8 9 [/SUP], Erika L Pearce[SUP] #[/SUP][SUP] 10 11 [/SUP]
Affiliations
- PMID: 33837219
- DOI: 10.1038/s41467-021-22449-w
Abstract
Our understanding of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still developing. We perform an observational study to investigate seroprevalence and immune responses in subjects professionally exposed to SARS-CoV-2 and their family members (155 individuals; ages 5-79 years). Seropositivity for SARS-CoV-2 Spike glycoprotein aligns with PCR results that confirm the previous infection. Anti-Spike IgG/IgM titers remain high 60 days post-infection and do not strongly associate with symptoms, except for fever. We analyze PBMCs from a subset of seropositive and seronegative adults. TLR7 agonist-activation reveals an increased population of IL-6[SUP]+[/SUP]TNF[SUP]-[/SUP]IL-1?[SUP]+[/SUP] monocytes, while SARS-CoV-2 peptide stimulation elicits IL-33, IL-6, IFNa2, and IL-23 expression in seropositive individuals. IL-33 correlates with CD4[SUP]+[/SUP] T cell activation in PBMCs from convalescent subjects and is likely due to T cell-mediated effects on IL-33-producing cells. IL-33 is associated with pulmonary infection and chronic diseases like asthma and COPD, but its role in COVID-19 is unknown. Analysis of published scRNAseq data of bronchoalveolar lavage fluid (BALF) from patients with mild to severe COVID-19 reveals a population of IL-33-producing cells that increases with the disease. Together these findings show that IL-33 production is linked to SARS-CoV-2 infection and warrant further investigation of IL-33 in COVID-19 pathogenesis and immunity.