tetano
Editor, Senior Moderator
Nat Commun
. 2022 Nov 12;13(1):6905.
doi: 10.1038/s41467-022-34728-1.
Durability and cross-reactivity of immune responses induced by a plant-based virus-like particle vaccine for COVID-19
Philipe Gobeil[SUP] 1 [/SUP], Stéphane Pillet[SUP] 1 [/SUP], Iohann Boulay[SUP] 1 [/SUP], Nathalie Charland[SUP] 1 [/SUP], Aurélien Lorin[SUP] 1 [/SUP], Matthew P Cheng[SUP] 2 [/SUP], Donald C Vinh[SUP] 2 [/SUP], Philippe Boutet[SUP] 3 [/SUP], Robbert Van Der Most[SUP] 4 5 [/SUP], François Roman[SUP] 3 [/SUP], Maria Angeles Ceregido[SUP] 3 [/SUP], Nathalie Landry[SUP] 1 [/SUP], Marc-André D'Aoust[SUP] 1 [/SUP], Brian J Ward[SUP] 6 7 [/SUP]
Affiliations
Abstract
As the SARS-CoV-2 pandemic evolves, vaccine evaluation needs to include consideration of both durability and cross-reactivity. This report expands on previously reported results from a Phase 1 trial of an AS03-adjuvanted, plant-based coronavirus-like particle (CoVLP) displaying the spike (S) glycoprotein of the ancestral SARS-CoV-2 virus in healthy adults (NCT04450004). Humoral and cellular responses against the ancestral strain were evaluated 6 months post-second dose (D201) as secondary outcomes. Independent of dose, all vaccinated individuals retain binding antibodies, and ~95% retain neutralizing antibodies (NAb). Interferon gamma and interleukin-4 responses remain detectable in ~94% and ~92% of vaccinees respectively. In post-hoc analyses, variant-specific (Alpha, Beta, Delta, Gamma and Omicron) NAb were assessed at D42 and D201. Using a live virus neutralization assay, broad cross-reactivity is detectable against all variants at D42. At D201, cross-reactive antibodies are detectable in almost all participants against Alpha, Gamma and Delta variants (94%) and the Beta variant (83%) and in a smaller proportion against Omicron (44%). Results are similar with the pseudovirion assay. These data suggest that two doses of 3.75 µg CoVLP+AS03 elicit a durable and cross-reactive response that persists for at least 6 months post-vaccination.
. 2022 Nov 12;13(1):6905.
doi: 10.1038/s41467-022-34728-1.
Durability and cross-reactivity of immune responses induced by a plant-based virus-like particle vaccine for COVID-19
Philipe Gobeil[SUP] 1 [/SUP], Stéphane Pillet[SUP] 1 [/SUP], Iohann Boulay[SUP] 1 [/SUP], Nathalie Charland[SUP] 1 [/SUP], Aurélien Lorin[SUP] 1 [/SUP], Matthew P Cheng[SUP] 2 [/SUP], Donald C Vinh[SUP] 2 [/SUP], Philippe Boutet[SUP] 3 [/SUP], Robbert Van Der Most[SUP] 4 5 [/SUP], François Roman[SUP] 3 [/SUP], Maria Angeles Ceregido[SUP] 3 [/SUP], Nathalie Landry[SUP] 1 [/SUP], Marc-André D'Aoust[SUP] 1 [/SUP], Brian J Ward[SUP] 6 7 [/SUP]
Affiliations
- PMID: 36371408
- DOI: 10.1038/s41467-022-34728-1
Abstract
As the SARS-CoV-2 pandemic evolves, vaccine evaluation needs to include consideration of both durability and cross-reactivity. This report expands on previously reported results from a Phase 1 trial of an AS03-adjuvanted, plant-based coronavirus-like particle (CoVLP) displaying the spike (S) glycoprotein of the ancestral SARS-CoV-2 virus in healthy adults (NCT04450004). Humoral and cellular responses against the ancestral strain were evaluated 6 months post-second dose (D201) as secondary outcomes. Independent of dose, all vaccinated individuals retain binding antibodies, and ~95% retain neutralizing antibodies (NAb). Interferon gamma and interleukin-4 responses remain detectable in ~94% and ~92% of vaccinees respectively. In post-hoc analyses, variant-specific (Alpha, Beta, Delta, Gamma and Omicron) NAb were assessed at D42 and D201. Using a live virus neutralization assay, broad cross-reactivity is detectable against all variants at D42. At D201, cross-reactive antibodies are detectable in almost all participants against Alpha, Gamma and Delta variants (94%) and the Beta variant (83%) and in a smaller proportion against Omicron (44%). Results are similar with the pseudovirion assay. These data suggest that two doses of 3.75 µg CoVLP+AS03 elicit a durable and cross-reactive response that persists for at least 6 months post-vaccination.