tetano
Editor, Senior Moderator
Nat Commun
. 2020 Dec 2;11(1):6176.
doi: 10.1038/s41467-020-19911-6.
Disposable silicon-based all-in-one micro-qPCR for apid on-site detection of pathogens
Estefania Nunez-Bajo[SUP] 1 [/SUP], Alexander Silva Pinto Collins[SUP] 1 [/SUP], Michael Kasimatis[SUP] 1 [/SUP], Yasin Cotur[SUP] 1 [/SUP], Tarek Asfour[SUP] 1 [/SUP], Ugur Tanriverdi[SUP] 1 [/SUP], Max Grell[SUP] 1 [/SUP], Matti Kaisti[SUP] 1 2 [/SUP], Guglielmo Senesi[SUP] 1 [/SUP], Karen Stevenson[SUP] 3 [/SUP], Firat G?der[SUP] 4 [/SUP]
Affiliations
Abstract
Rapid screening and low-cost diagnosis play a crucial role in choosing the correct course of intervention when dealing with highly infectious pathogens. This is especially important if the disease-causing agent has no effective treatment, such as the novel coronavirus SARS-CoV-2, and shows no or similar symptoms to other common infections. Here, we report a disposable silicon-based integrated Point-of-Need transducer (TriSilix) for real-time quantitative detection of pathogen-specific sequences of nucleic acids. TriSilix can be produced at wafer-scale in a standard laboratory (37 chips of 10 ? 10 ? 0.65 mm in size can be produced in 7 h, costing ~0.35 USD per device). We are able to quantitatively detect a 563 bp fragment of genomic DNA of Mycobacterium avium subspecies paratuberculosis through real-time PCR with a limit-of-detection of 20 fg, equivalent to a single bacterium, at the 35[SUP]th[/SUP] cycle. Using TriSilix, we also detect the cDNA from SARS-CoV-2 (1 pg) with high specificity against SARS-CoV (2003).
. 2020 Dec 2;11(1):6176.
doi: 10.1038/s41467-020-19911-6.
Disposable silicon-based all-in-one micro-qPCR for apid on-site detection of pathogens
Estefania Nunez-Bajo[SUP] 1 [/SUP], Alexander Silva Pinto Collins[SUP] 1 [/SUP], Michael Kasimatis[SUP] 1 [/SUP], Yasin Cotur[SUP] 1 [/SUP], Tarek Asfour[SUP] 1 [/SUP], Ugur Tanriverdi[SUP] 1 [/SUP], Max Grell[SUP] 1 [/SUP], Matti Kaisti[SUP] 1 2 [/SUP], Guglielmo Senesi[SUP] 1 [/SUP], Karen Stevenson[SUP] 3 [/SUP], Firat G?der[SUP] 4 [/SUP]
Affiliations
- PMID: 33268779
- DOI: 10.1038/s41467-020-19911-6
Abstract
Rapid screening and low-cost diagnosis play a crucial role in choosing the correct course of intervention when dealing with highly infectious pathogens. This is especially important if the disease-causing agent has no effective treatment, such as the novel coronavirus SARS-CoV-2, and shows no or similar symptoms to other common infections. Here, we report a disposable silicon-based integrated Point-of-Need transducer (TriSilix) for real-time quantitative detection of pathogen-specific sequences of nucleic acids. TriSilix can be produced at wafer-scale in a standard laboratory (37 chips of 10 ? 10 ? 0.65 mm in size can be produced in 7 h, costing ~0.35 USD per device). We are able to quantitatively detect a 563 bp fragment of genomic DNA of Mycobacterium avium subspecies paratuberculosis through real-time PCR with a limit-of-detection of 20 fg, equivalent to a single bacterium, at the 35[SUP]th[/SUP] cycle. Using TriSilix, we also detect the cDNA from SARS-CoV-2 (1 pg) with high specificity against SARS-CoV (2003).