tetano
Editor, Senior Moderator
Nat Commun
. 2022 Jul 21;13(1):4212.
doi: 10.1038/s41467-022-31796-1.
Detection of neutralizing antibodies against multiple SARS-CoV-2 strains in dried blood spots using cell-free PCR
Kenneth Danh[SUP] 1 [/SUP], Donna Grace Karp[SUP] 1 [/SUP], Malvika Singhal[SUP] 1 [/SUP], Akshaya Tankasala[SUP] 1 [/SUP], David Gebhart[SUP] 1 [/SUP], Felipe de Jesus Cortez[SUP] 1 [/SUP], Devangkumar Tandel[SUP] 1 [/SUP], Peter V Robinson[SUP] 1 [/SUP], David Seftel[SUP] 1 [/SUP], Mars Stone[SUP] 2 [/SUP], Graham Simmons[SUP] 2 [/SUP], Anil Bagri[SUP] 3 [/SUP], Martin A Schreiber[SUP] 4 [/SUP], Andreas Buser[SUP] 5 [/SUP], Andreas Holbro[SUP] 5 [/SUP], Manuel Battegay[SUP] 6 [/SUP], Mary Kate Morris[SUP] 7 [/SUP], Carl Hanson[SUP] 7 [/SUP], John R Mills[SUP] 8 [/SUP], Dane Granger[SUP] 8 [/SUP], Elitza S Theel[SUP] 8 [/SUP], James R Stubbs[SUP] 8 [/SUP], Laurence M Corash[SUP] 3 [/SUP], Cheng-Ting Tsai[SUP] 9 [/SUP]
Affiliations
Abstract
An easily implementable serological assay to accurately detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) neutralizing antibodies is urgently needed to better track herd immunity, vaccine efficacy and vaccination rates. Herein, we report the Split-Oligonucleotide Neighboring Inhibition Assay (SONIA) which uses real-time qPCR to measure the ability of neutralizing antibodies to block binding between DNA-barcoded viral spike protein subunit 1 and the human angiotensin-converting enzyme 2 receptor protein. The SONIA neutralizing antibody assay using finger-prick dried blood spots displays 91-97% sensitivity and 100% specificity in comparison to the live-virus neutralization assays using matched serum specimens for multiple SARS-CoV-2 variants-of-concern. The multiplex version of this neutralizing antibody assay, using easily collectable finger-prick dried blood spots, can be a valuable tool to help reveal the impact of age, pre-existing health conditions, waning immunity, different vaccination schemes and the emergence of new variants-of-concern.
. 2022 Jul 21;13(1):4212.
doi: 10.1038/s41467-022-31796-1.
Detection of neutralizing antibodies against multiple SARS-CoV-2 strains in dried blood spots using cell-free PCR
Kenneth Danh[SUP] 1 [/SUP], Donna Grace Karp[SUP] 1 [/SUP], Malvika Singhal[SUP] 1 [/SUP], Akshaya Tankasala[SUP] 1 [/SUP], David Gebhart[SUP] 1 [/SUP], Felipe de Jesus Cortez[SUP] 1 [/SUP], Devangkumar Tandel[SUP] 1 [/SUP], Peter V Robinson[SUP] 1 [/SUP], David Seftel[SUP] 1 [/SUP], Mars Stone[SUP] 2 [/SUP], Graham Simmons[SUP] 2 [/SUP], Anil Bagri[SUP] 3 [/SUP], Martin A Schreiber[SUP] 4 [/SUP], Andreas Buser[SUP] 5 [/SUP], Andreas Holbro[SUP] 5 [/SUP], Manuel Battegay[SUP] 6 [/SUP], Mary Kate Morris[SUP] 7 [/SUP], Carl Hanson[SUP] 7 [/SUP], John R Mills[SUP] 8 [/SUP], Dane Granger[SUP] 8 [/SUP], Elitza S Theel[SUP] 8 [/SUP], James R Stubbs[SUP] 8 [/SUP], Laurence M Corash[SUP] 3 [/SUP], Cheng-Ting Tsai[SUP] 9 [/SUP]
Affiliations
- PMID: 35864104
- DOI: 10.1038/s41467-022-31796-1
Abstract
An easily implementable serological assay to accurately detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) neutralizing antibodies is urgently needed to better track herd immunity, vaccine efficacy and vaccination rates. Herein, we report the Split-Oligonucleotide Neighboring Inhibition Assay (SONIA) which uses real-time qPCR to measure the ability of neutralizing antibodies to block binding between DNA-barcoded viral spike protein subunit 1 and the human angiotensin-converting enzyme 2 receptor protein. The SONIA neutralizing antibody assay using finger-prick dried blood spots displays 91-97% sensitivity and 100% specificity in comparison to the live-virus neutralization assays using matched serum specimens for multiple SARS-CoV-2 variants-of-concern. The multiplex version of this neutralizing antibody assay, using easily collectable finger-prick dried blood spots, can be a valuable tool to help reveal the impact of age, pre-existing health conditions, waning immunity, different vaccination schemes and the emergence of new variants-of-concern.