tetano
Editor, Senior Moderator
Nat Commun
. 2024 Aug 9;15(1):6811.
doi: 10.1038/s41467-024-50997-4. Atypical and non-classical CD45RB[SUP]lo[/SUP] memory B cells are the majority of circulating SARS-CoV-2 specific B cells following mRNA vaccination or COVID-19
David G Priest[SUP] 1 [/SUP], Takeshi Ebihara[SUP] 2 3 [/SUP], Janyerkye Tulyeu[SUP] 4 [/SUP], Jonas N Søndergaard[SUP] 4 [/SUP], Shuhei Sakakibara[SUP] 5 6 [/SUP], Fuminori Sugihara[SUP] 7 8 [/SUP], Shunichiro Nakao[SUP] 3 [/SUP], Yuki Togami[SUP] 3 [/SUP], Jumpei Yoshimura[SUP] 3 [/SUP], Hiroshi Ito[SUP] 3 [/SUP], Shinya Onishi[SUP] 3 [/SUP], Arisa Muratsu[SUP] 3 [/SUP], Yumi Mitsuyama[SUP] 3 9 [/SUP], Hiroshi Ogura[SUP] 2 3 [/SUP], Jun Oda[SUP] 2 3 [/SUP], Daisuke Okusaki[SUP] 2 10 11 12 [/SUP], Hisatake Matsumoto[SUP] 13 14 [/SUP], James B Wing[SUP] 15 16 17 [/SUP]
Affiliations
Resting memory B cells can be divided into classical or atypical groups, but the heterogenous marker expression on activated memory B cells makes similar classification difficult. Here, by longitudinal analysis of mass cytometry and CITE-seq data from cohorts with COVID-19, bacterial sepsis, or BNT162b2 mRNA vaccine, we observe that resting B cell memory consist of classical CD45RB[SUP]+[/SUP] memory and CD45RB[SUP]lo[/SUP] memory, of which the latter contains of two distinct groups of CD11c[SUP]+[/SUP] atypical and CD23[SUP]+[/SUP] non-classical memory cells. CD45RB levels remain stable in these cells after activation, thereby enabling the tracking of activated B cells and plasmablasts derived from either CD45RB[SUP]+[/SUP] or CD45RB[SUP]lo[/SUP] memory B cells. Moreover, in both COVID-19 patients and mRNA vaccination, CD45RB[SUP]lo[/SUP] B cells formed the majority of SARS-CoV2 specific memory B cells and correlated with serum antibodies, while CD45RB[SUP]+[/SUP] memory are activated by bacterial sepsis. Our results thus identify that stably expressed CD45RB levels can be exploited to trace resting memory B cells and their activated progeny, and suggest that atypical and non-classical CD45RB[SUP]lo[/SUP] memory B cells contribute to SARS-CoV-2 infection and vaccination.
. 2024 Aug 9;15(1):6811.
doi: 10.1038/s41467-024-50997-4. Atypical and non-classical CD45RB[SUP]lo[/SUP] memory B cells are the majority of circulating SARS-CoV-2 specific B cells following mRNA vaccination or COVID-19
David G Priest[SUP] 1 [/SUP], Takeshi Ebihara[SUP] 2 3 [/SUP], Janyerkye Tulyeu[SUP] 4 [/SUP], Jonas N Søndergaard[SUP] 4 [/SUP], Shuhei Sakakibara[SUP] 5 6 [/SUP], Fuminori Sugihara[SUP] 7 8 [/SUP], Shunichiro Nakao[SUP] 3 [/SUP], Yuki Togami[SUP] 3 [/SUP], Jumpei Yoshimura[SUP] 3 [/SUP], Hiroshi Ito[SUP] 3 [/SUP], Shinya Onishi[SUP] 3 [/SUP], Arisa Muratsu[SUP] 3 [/SUP], Yumi Mitsuyama[SUP] 3 9 [/SUP], Hiroshi Ogura[SUP] 2 3 [/SUP], Jun Oda[SUP] 2 3 [/SUP], Daisuke Okusaki[SUP] 2 10 11 12 [/SUP], Hisatake Matsumoto[SUP] 13 14 [/SUP], James B Wing[SUP] 15 16 17 [/SUP]
Affiliations
- PMID: 39122676
- PMCID: PMC11315995
- DOI: 10.1038/s41467-024-50997-4
Resting memory B cells can be divided into classical or atypical groups, but the heterogenous marker expression on activated memory B cells makes similar classification difficult. Here, by longitudinal analysis of mass cytometry and CITE-seq data from cohorts with COVID-19, bacterial sepsis, or BNT162b2 mRNA vaccine, we observe that resting B cell memory consist of classical CD45RB[SUP]+[/SUP] memory and CD45RB[SUP]lo[/SUP] memory, of which the latter contains of two distinct groups of CD11c[SUP]+[/SUP] atypical and CD23[SUP]+[/SUP] non-classical memory cells. CD45RB levels remain stable in these cells after activation, thereby enabling the tracking of activated B cells and plasmablasts derived from either CD45RB[SUP]+[/SUP] or CD45RB[SUP]lo[/SUP] memory B cells. Moreover, in both COVID-19 patients and mRNA vaccination, CD45RB[SUP]lo[/SUP] B cells formed the majority of SARS-CoV2 specific memory B cells and correlated with serum antibodies, while CD45RB[SUP]+[/SUP] memory are activated by bacterial sepsis. Our results thus identify that stably expressed CD45RB levels can be exploited to trace resting memory B cells and their activated progeny, and suggest that atypical and non-classical CD45RB[SUP]lo[/SUP] memory B cells contribute to SARS-CoV-2 infection and vaccination.