tetano
Editor, Senior Moderator
Nat Commun. 2014 Dec 17;5:5750. doi: 10.1038/ncomms6750.
[h=1]Ferrets exclusively synthesize Neu5Ac and express naturally humanized influenza A virus receptors.[/h] Ng PS[SUP]1[/SUP], B?hm R[SUP]1[/SUP], Hartley-Tassell LE[SUP]1[/SUP], Steen JA[SUP]2[/SUP], Wang H, Lukowski SW[SUP]3[/SUP], Hawthorne PL, Trezise AE, Coloe PJ[SUP]4[/SUP], Grimmond SM[SUP]5[/SUP], Haselhorst T[SUP]1[/SUP], von Itzstein M[SUP]1[/SUP], Paton AW, Paton JC, Jennings MP[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Mammals express the sialic acids N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc) on cell surfaces, where they act as receptors for pathogens, including influenza A virus (IAV). Neu5Gc is synthesized from Neu5Ac by the enzyme cytidine monophosphate-N-acetylneuraminic acid hydroxylase (CMAH). In humans, this enzyme is inactive and only Neu5Ac is produced. Ferrets are susceptible to human-adapted IAV strains and have been the dominant animal model for IAV studies. Here we show that ferrets, like humans, do not synthesize Neu5Gc. Genomic analysis reveals an ancient, nine-exon deletion in the ferret CMAH gene that is shared by the Pinnipedia and Musteloidia members of the Carnivora. Interactions between two human strains of IAV with the sialyllactose receptor (sialic acid-α2,6Gal) confirm that the type of terminal sialic acid contributes significantly to IAV receptor specificity. Our results indicate that exclusive expression of Neu5Ac contributes to the susceptibility of ferrets to human-adapted IAV strains.
PMID: 25517696 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25517696
[h=1]Ferrets exclusively synthesize Neu5Ac and express naturally humanized influenza A virus receptors.[/h] Ng PS[SUP]1[/SUP], B?hm R[SUP]1[/SUP], Hartley-Tassell LE[SUP]1[/SUP], Steen JA[SUP]2[/SUP], Wang H, Lukowski SW[SUP]3[/SUP], Hawthorne PL, Trezise AE, Coloe PJ[SUP]4[/SUP], Grimmond SM[SUP]5[/SUP], Haselhorst T[SUP]1[/SUP], von Itzstein M[SUP]1[/SUP], Paton AW, Paton JC, Jennings MP[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Mammals express the sialic acids N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc) on cell surfaces, where they act as receptors for pathogens, including influenza A virus (IAV). Neu5Gc is synthesized from Neu5Ac by the enzyme cytidine monophosphate-N-acetylneuraminic acid hydroxylase (CMAH). In humans, this enzyme is inactive and only Neu5Ac is produced. Ferrets are susceptible to human-adapted IAV strains and have been the dominant animal model for IAV studies. Here we show that ferrets, like humans, do not synthesize Neu5Gc. Genomic analysis reveals an ancient, nine-exon deletion in the ferret CMAH gene that is shared by the Pinnipedia and Musteloidia members of the Carnivora. Interactions between two human strains of IAV with the sialyllactose receptor (sialic acid-α2,6Gal) confirm that the type of terminal sialic acid contributes significantly to IAV receptor specificity. Our results indicate that exclusive expression of Neu5Ac contributes to the susceptibility of ferrets to human-adapted IAV strains.
PMID: 25517696 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25517696
