tetano
Editor, Senior Moderator
Nat Commun. 2014 Jul 23;5:4448. doi: 10.1038/ncomms5448.
An infectious bat-derived chimeric influenza virus harbouring the entry machinery of an influenza A virus.
Juozapaitis M1, Aguiar Moreira E2, Mena I3, Giese S4, Riegger D5, Pohlmann A6, H?per D6, Zimmer G7, Beer M6, Garc?a-Sastre A8, Schwemmle M5.
Author information
Abstract
In 2012, the complete genomic sequence of a new and potentially harmful influenza A-like virus from bats (H17N10) was identified. However, infectious influenza virus was neither isolated from infected bats nor reconstituted, impeding further characterization of this virus. Here we show the generation of an infectious chimeric virus containing six out of the eight bat virus genes, with the remaining two genes encoding the haemagglutinin and neuraminidase proteins of a prototypic influenza A virus. This engineered virus replicates well in a broad range of mammalian cell cultures, human primary airway epithelial cells and mice, but poorly in avian cells and chicken embryos without further adaptation. Importantly, the bat chimeric virus is unable to reassort with other influenza A viruses. Although our data do not exclude the possibility of zoonotic transmission of bat influenza viruses into the human population, they indicate that multiple barriers exist that makes this an unlikely event.
PMID:
25055345
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/25055345
An infectious bat-derived chimeric influenza virus harbouring the entry machinery of an influenza A virus.
Juozapaitis M1, Aguiar Moreira E2, Mena I3, Giese S4, Riegger D5, Pohlmann A6, H?per D6, Zimmer G7, Beer M6, Garc?a-Sastre A8, Schwemmle M5.
Author information
Abstract
In 2012, the complete genomic sequence of a new and potentially harmful influenza A-like virus from bats (H17N10) was identified. However, infectious influenza virus was neither isolated from infected bats nor reconstituted, impeding further characterization of this virus. Here we show the generation of an infectious chimeric virus containing six out of the eight bat virus genes, with the remaining two genes encoding the haemagglutinin and neuraminidase proteins of a prototypic influenza A virus. This engineered virus replicates well in a broad range of mammalian cell cultures, human primary airway epithelial cells and mice, but poorly in avian cells and chicken embryos without further adaptation. Importantly, the bat chimeric virus is unable to reassort with other influenza A viruses. Although our data do not exclude the possibility of zoonotic transmission of bat influenza viruses into the human population, they indicate that multiple barriers exist that makes this an unlikely event.
PMID:
25055345
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/25055345