tetano
Editor, Senior Moderator
Nat Chem
. 2021 Mar 22.
doi: 10.1038/s41557-021-00655-9. Online ahead of print.
Synthetic O-acetylated sialosides facilitate functional receptor identification for human respiratory viruses
Zeshi Li[SUP] #[/SUP][SUP] 1 [/SUP], Yifei Lang[SUP] #[/SUP][SUP] 2 [/SUP], Lin Liu[SUP] 3 [/SUP], Mehman I Bunyatov[SUP] 1 [/SUP], Angelic Isaza Sarmiento[SUP] 2 [/SUP], Raoul J de Groot[SUP] 4 [/SUP], Geert-Jan Boons[SUP] 5 6 7 8 [/SUP]
Affiliations
Abstract
The transmission of viruses from animal reservoirs to humans poses major threats to public health. Preparedness for future zoonotic outbreaks requires a fundamental understanding of how viruses of animal origin have adapted to binding to a cell surface component and/or receptor of the new host. Here we report on the specificities of human and animal viruses that engage with O-acetylated sialic acid, which include betacoronaviruses, toroviruses and influenza C and D viruses. Key to these studies was the development of a chemoenzymatic methodology that can provide almost any sialate-acetylation pattern. A collection of O-acetylated sialoglycans was printed as a microarray for the determination of receptor specificity. These studies showed host-specific patterns of receptor recognition and revealed that three distinct human respiratory viruses uniquely bind 9-O-acetylated ?2,8-linked disialoside. Immunofluorescence and cell entry studies support that such a glycotope as part of a ganglioside is a functional receptor for human coronaviruses.
. 2021 Mar 22.
doi: 10.1038/s41557-021-00655-9. Online ahead of print.
Synthetic O-acetylated sialosides facilitate functional receptor identification for human respiratory viruses
Zeshi Li[SUP] #[/SUP][SUP] 1 [/SUP], Yifei Lang[SUP] #[/SUP][SUP] 2 [/SUP], Lin Liu[SUP] 3 [/SUP], Mehman I Bunyatov[SUP] 1 [/SUP], Angelic Isaza Sarmiento[SUP] 2 [/SUP], Raoul J de Groot[SUP] 4 [/SUP], Geert-Jan Boons[SUP] 5 6 7 8 [/SUP]
Affiliations
- PMID: 33753916
- DOI: 10.1038/s41557-021-00655-9
Abstract
The transmission of viruses from animal reservoirs to humans poses major threats to public health. Preparedness for future zoonotic outbreaks requires a fundamental understanding of how viruses of animal origin have adapted to binding to a cell surface component and/or receptor of the new host. Here we report on the specificities of human and animal viruses that engage with O-acetylated sialic acid, which include betacoronaviruses, toroviruses and influenza C and D viruses. Key to these studies was the development of a chemoenzymatic methodology that can provide almost any sialate-acetylation pattern. A collection of O-acetylated sialoglycans was printed as a microarray for the determination of receptor specificity. These studies showed host-specific patterns of receptor recognition and revealed that three distinct human respiratory viruses uniquely bind 9-O-acetylated ?2,8-linked disialoside. Immunofluorescence and cell entry studies support that such a glycotope as part of a ganglioside is a functional receptor for human coronaviruses.