tetano
Editor, Senior Moderator
Nat Cell Biol
. 2021 Jun;23(6):620-630.
doi: 10.1038/s41556-021-00690-1. Epub 2021 Jun 9.
Single-cell epigenomic landscape of peripheral immune cells reveals establishment of trained immunity in individuals convalescing from COVID-19
Maojun You[SUP] #[/SUP][SUP] 1 2 [/SUP], Liang Chen[SUP] #[/SUP][SUP] 3 4 5 6 [/SUP], Dawei Zhang[SUP] #[/SUP][SUP] 7 [/SUP], Peng Zhao[SUP] #[/SUP][SUP] 7 [/SUP], Zhu Chen[SUP] 7 [/SUP], En-Qiang Qin[SUP] 7 [/SUP], Yanan Gao[SUP] 1 2 [/SUP], Mark M Davis[SUP] 8 9 10 [/SUP], Pengyuan Yang[SUP] 11 12 [/SUP]
Affiliations
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection often causes severe complications and even death. However, asymptomatic infection has also been reported, highlighting the difference in immune responses among individuals. Here we performed single-cell chromatin accessibility and T cell-receptor analyses of peripheral blood mononuclear cells collected from individuals convalescing from COVID-19 and healthy donors. Chromatin remodelling was observed in both innate and adaptive immune cells in the individuals convalescing from COVID-19. Compared with healthy donors, recovered individuals contained abundant TBET-enriched CD16[SUP]+[/SUP] and IRF1-enriched CD14[SUP]+[/SUP] monocytes with sequential trained and activated epigenomic states. The B-cell lineage in recovered individuals exhibited an accelerated developmental programme from immature B cells to antibody-producing plasma cells. Finally, an integrated analysis of single-cell T cell-receptor clonality with the chromatin accessibility landscape revealed the expansion of putative SARS-CoV-2-specific CD8[SUP]+[/SUP] T cells with epigenomic profiles that promote the differentiation of effector or memory cells. Overall, our data suggest that immune cells of individuals convalescing from COVID-19 exhibit global remodelling of the chromatin accessibility landscape, indicative of the establishment of immunological memory.
. 2021 Jun;23(6):620-630.
doi: 10.1038/s41556-021-00690-1. Epub 2021 Jun 9.
Single-cell epigenomic landscape of peripheral immune cells reveals establishment of trained immunity in individuals convalescing from COVID-19
Maojun You[SUP] #[/SUP][SUP] 1 2 [/SUP], Liang Chen[SUP] #[/SUP][SUP] 3 4 5 6 [/SUP], Dawei Zhang[SUP] #[/SUP][SUP] 7 [/SUP], Peng Zhao[SUP] #[/SUP][SUP] 7 [/SUP], Zhu Chen[SUP] 7 [/SUP], En-Qiang Qin[SUP] 7 [/SUP], Yanan Gao[SUP] 1 2 [/SUP], Mark M Davis[SUP] 8 9 10 [/SUP], Pengyuan Yang[SUP] 11 12 [/SUP]
Affiliations
- PMID: 34108657
- DOI: 10.1038/s41556-021-00690-1
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection often causes severe complications and even death. However, asymptomatic infection has also been reported, highlighting the difference in immune responses among individuals. Here we performed single-cell chromatin accessibility and T cell-receptor analyses of peripheral blood mononuclear cells collected from individuals convalescing from COVID-19 and healthy donors. Chromatin remodelling was observed in both innate and adaptive immune cells in the individuals convalescing from COVID-19. Compared with healthy donors, recovered individuals contained abundant TBET-enriched CD16[SUP]+[/SUP] and IRF1-enriched CD14[SUP]+[/SUP] monocytes with sequential trained and activated epigenomic states. The B-cell lineage in recovered individuals exhibited an accelerated developmental programme from immature B cells to antibody-producing plasma cells. Finally, an integrated analysis of single-cell T cell-receptor clonality with the chromatin accessibility landscape revealed the expansion of putative SARS-CoV-2-specific CD8[SUP]+[/SUP] T cells with epigenomic profiles that promote the differentiation of effector or memory cells. Overall, our data suggest that immune cells of individuals convalescing from COVID-19 exhibit global remodelling of the chromatin accessibility landscape, indicative of the establishment of immunological memory.