tetano
Editor, Senior Moderator
Nat Biotechnol
. 2020 Sep 18.
doi: 10.1038/s41587-020-0684-z. Online ahead of print.
Measurement of SARS-CoV-2 RNA in wastewater tracks community infection dynamics
Jordan Peccia[SUP] 1 [/SUP], Alessandro Zulli[SUP] 2 [/SUP], Doug E Brackney[SUP] 3 [/SUP], Nathan D Grubaugh[SUP] 4 [/SUP], Edward H Kaplan[SUP] 2 5 6 [/SUP], Arnau Casanovas-Massana[SUP] 4 [/SUP], Albert I Ko[SUP] 4 [/SUP], Amyn A Malik[SUP] 7 8 [/SUP], Dennis Wang[SUP] 7 [/SUP], Mike Wang[SUP] 7 [/SUP], Joshua L Warren[SUP] 9 [/SUP], Daniel M Weinberger[SUP] 4 [/SUP], Wyatt Arnold[SUP] 2 [/SUP], Saad B Omer[SUP] 10 11 12 13 [/SUP]
Affiliations
Abstract
We measured severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA concentrations in primary sewage sludge in the New Haven, Connecticut, USA, metropolitan area during the Coronavirus Disease 2019 (COVID-19) outbreak in Spring 2020. SARS-CoV-2 RNA was detected throughout the more than 10-week study and, when adjusted for time lags, tracked the rise and fall of cases seen in SARS-CoV-2 clinical test results and local COVID-19 hospital admissions. Relative to these indicators, SARS-CoV-2 RNA concentrations in sludge were 0-2 d ahead of SARS-CoV-2 positive test results by date of specimen collection, 0-2 d ahead of the percentage of positive tests by date of specimen collection, 1-4 d ahead of local hospital admissions and 6-8 d ahead of SARS-CoV-2 positive test results by reporting date. Our data show the utility of viral RNA monitoring in municipal wastewater for SARS-CoV-2 infection surveillance at a population-wide level. In communities facing a delay between specimen collection and the reporting of test results, immediate wastewater results can provide considerable advance notice of infection dynamics.
. 2020 Sep 18.
doi: 10.1038/s41587-020-0684-z. Online ahead of print.
Measurement of SARS-CoV-2 RNA in wastewater tracks community infection dynamics
Jordan Peccia[SUP] 1 [/SUP], Alessandro Zulli[SUP] 2 [/SUP], Doug E Brackney[SUP] 3 [/SUP], Nathan D Grubaugh[SUP] 4 [/SUP], Edward H Kaplan[SUP] 2 5 6 [/SUP], Arnau Casanovas-Massana[SUP] 4 [/SUP], Albert I Ko[SUP] 4 [/SUP], Amyn A Malik[SUP] 7 8 [/SUP], Dennis Wang[SUP] 7 [/SUP], Mike Wang[SUP] 7 [/SUP], Joshua L Warren[SUP] 9 [/SUP], Daniel M Weinberger[SUP] 4 [/SUP], Wyatt Arnold[SUP] 2 [/SUP], Saad B Omer[SUP] 10 11 12 13 [/SUP]
Affiliations
- PMID: 32948856
- DOI: 10.1038/s41587-020-0684-z
Abstract
We measured severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA concentrations in primary sewage sludge in the New Haven, Connecticut, USA, metropolitan area during the Coronavirus Disease 2019 (COVID-19) outbreak in Spring 2020. SARS-CoV-2 RNA was detected throughout the more than 10-week study and, when adjusted for time lags, tracked the rise and fall of cases seen in SARS-CoV-2 clinical test results and local COVID-19 hospital admissions. Relative to these indicators, SARS-CoV-2 RNA concentrations in sludge were 0-2 d ahead of SARS-CoV-2 positive test results by date of specimen collection, 0-2 d ahead of the percentage of positive tests by date of specimen collection, 1-4 d ahead of local hospital admissions and 6-8 d ahead of SARS-CoV-2 positive test results by reporting date. Our data show the utility of viral RNA monitoring in municipal wastewater for SARS-CoV-2 infection surveillance at a population-wide level. In communities facing a delay between specimen collection and the reporting of test results, immediate wastewater results can provide considerable advance notice of infection dynamics.