tetano
Editor, Senior Moderator
Nat Biomed Eng
. 2023 Sep 21.
doi: 10.1038/s41551-023-01092-4. Online ahead of print. Multiplexed discrimination of SARS-CoV-2 variants via plasmonic-enhanced fluorescence in a portable and automated device
Ying Liu[SUP] #[/SUP][SUP] 1 [/SUP], Yang Yang[SUP] #[/SUP][SUP] 2 [/SUP], Guanghui Wang[SUP] #[/SUP][SUP] 1 [/SUP], Dou Wang[SUP] #[/SUP][SUP] 1 [/SUP], Pan-Lin Shao[SUP] 3 [/SUP], Jiahu Tang[SUP] 1 [/SUP], Tingzhen He[SUP] 1 [/SUP], Jintao Zheng[SUP] 1 [/SUP], Ruibin Hu[SUP] 1 [/SUP], Yiyi Liu[SUP] 1 [/SUP], Ziyi Xu[SUP] 1 [/SUP], Dan Niu[SUP] 1 [/SUP], Jiahui Lv[SUP] 1 [/SUP], Jingkai Yang[SUP] 1 [/SUP], Hongjun Xiao[SUP] 1 [/SUP], Shuai Wu[SUP] 2 [/SUP], Shuang He[SUP] 1 [/SUP], Zhongrong Tang[SUP] 1 [/SUP], Yan Liu[SUP] 2 [/SUP], Meijie Tang[SUP] 4 [/SUP], Xingyu Jiang[SUP] 5 [/SUP], Jing Yuan[SUP] 6 [/SUP], Hongjie Dai[SUP] 7 [/SUP], Bo Zhang[SUP] 8 [/SUP]
Affiliations
Portable assays for the rapid identification of lineages of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are needed to aid large-scale efforts in monitoring the evolution of the virus. Here we report a multiplexed assay in a microarray format for the detection, via isothermal amplification and plasmonic-gold-enhanced near-infrared fluorescence, of variants of SARS-CoV-2. The assay, which has single-nucleotide specificity for variant discrimination, single-RNA-copy sensitivity and does not require RNA extraction, discriminated 12 lineages of SARS-CoV-2 (in three mutational hotspots of the Spike protein) and detected the virus in nasopharyngeal swabs from 1,034 individuals at 98.8% sensitivity and 100% specificity, with 97.6% concordance with genome sequencing in variant discrimination. We also report a compact, portable and fully automated device integrating the entire swab-to-result workflow and amenable to the point-of-care detection of SARS-CoV-2 variants. Portable, rapid, accurate and multiplexed assays for the detection of SARS-CoV-2 variants and lineages may facilitate variant-surveillance efforts.
. 2023 Sep 21.
doi: 10.1038/s41551-023-01092-4. Online ahead of print. Multiplexed discrimination of SARS-CoV-2 variants via plasmonic-enhanced fluorescence in a portable and automated device
Ying Liu[SUP] #[/SUP][SUP] 1 [/SUP], Yang Yang[SUP] #[/SUP][SUP] 2 [/SUP], Guanghui Wang[SUP] #[/SUP][SUP] 1 [/SUP], Dou Wang[SUP] #[/SUP][SUP] 1 [/SUP], Pan-Lin Shao[SUP] 3 [/SUP], Jiahu Tang[SUP] 1 [/SUP], Tingzhen He[SUP] 1 [/SUP], Jintao Zheng[SUP] 1 [/SUP], Ruibin Hu[SUP] 1 [/SUP], Yiyi Liu[SUP] 1 [/SUP], Ziyi Xu[SUP] 1 [/SUP], Dan Niu[SUP] 1 [/SUP], Jiahui Lv[SUP] 1 [/SUP], Jingkai Yang[SUP] 1 [/SUP], Hongjun Xiao[SUP] 1 [/SUP], Shuai Wu[SUP] 2 [/SUP], Shuang He[SUP] 1 [/SUP], Zhongrong Tang[SUP] 1 [/SUP], Yan Liu[SUP] 2 [/SUP], Meijie Tang[SUP] 4 [/SUP], Xingyu Jiang[SUP] 5 [/SUP], Jing Yuan[SUP] 6 [/SUP], Hongjie Dai[SUP] 7 [/SUP], Bo Zhang[SUP] 8 [/SUP]
Affiliations
- PMID: 37735541
- DOI: 10.1038/s41551-023-01092-4
Portable assays for the rapid identification of lineages of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are needed to aid large-scale efforts in monitoring the evolution of the virus. Here we report a multiplexed assay in a microarray format for the detection, via isothermal amplification and plasmonic-gold-enhanced near-infrared fluorescence, of variants of SARS-CoV-2. The assay, which has single-nucleotide specificity for variant discrimination, single-RNA-copy sensitivity and does not require RNA extraction, discriminated 12 lineages of SARS-CoV-2 (in three mutational hotspots of the Spike protein) and detected the virus in nasopharyngeal swabs from 1,034 individuals at 98.8% sensitivity and 100% specificity, with 97.6% concordance with genome sequencing in variant discrimination. We also report a compact, portable and fully automated device integrating the entire swab-to-result workflow and amenable to the point-of-care detection of SARS-CoV-2 variants. Portable, rapid, accurate and multiplexed assays for the detection of SARS-CoV-2 variants and lineages may facilitate variant-surveillance efforts.