tetano
Editor, Senior Moderator
Int Immunopharmacol. 2019 Dec 13;78:106115. doi: 10.1016/j.intimp.2019.106115. [Epub ahead of print] [h=1]Nasal priming with immunobiotic lactobacilli improves the adaptive immune response against influenza virus.[/h]
Tonetti FR[SUP]1[/SUP], Islam MA[SUP]2[/SUP], Vizoso-Pinto MG[SUP]1[/SUP], Takahashi H[SUP]3[/SUP], Kitazawa H[SUP]4[/SUP], Villena J[SUP]5[/SUP].
[h=3]Author information[/h] 1 Infection Biology Laboratory, INSIBIO (UNT-CONICET), Tucum?n, Argentina; Laboratory of Basic Sciences - Genetics, Faculty of Medicine, National University of Tucuman, Tucum?n, Argentina. 2 Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan. 3 Laboratory of Plant Pathology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan; Plant Immunology Unit, International Education and Research Center for Food Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan. 4 Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan; Livestock Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai, Japan. Electronic address: haruki.kitazawa.c7@tohoku.ac.jp. 5 Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan; Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucum?n, Argentina. Electronic address: jcvillena@cerela.org.ar.
[h=3]Abstract[/h] The nasal priming with Lactobacillus rhamnosus CRL1505 modulates the respiratory antiviral innate immune response and improves protection against influenza virus (IFV) challenge in mice. However, the potential beneficial effect of the CRL1505 strain on the adaptive immune response triggered by IFV infection or vaccination was not evaluated before. In this work, we demonstrated that nasally administered L. rhamnosus CRL1505 is able to improve both the humoral and cellular adaptive immune responses induced by IFV infection or vaccination. Higher levels of IFV-specific IgA and IgG as well as IFN-γ were found in the serum and the respiratory tract of CRL1505-treated mice after IFV challenge. Lactobacilli treated mice also showed reduced concentrations of IL-17 and improved levels of IL-10 during IFV infection. The differential balance of inflammatory and regulatory cytokines induced by L. rhamnosus CRL1505 contributed to the protection against IFV by favoring an effective effector immune response without inducing inflammatory-mediated lung damage. The optimal immunomodulatory effect of the CRL1505 strain was achieved with viable bacteria. However, non-viable L. rhamnosus CRL1505 was also efficient in improving the adaptive immune responses generated by IFV challenges and therefore, emerged as an interesting alternative for vaccination of immunocompromised hosts. Similar to other immunomodulatory properties of lactobacilli, it was shown here that the adjuvant effect in the context of IFV vaccination was a strain dependent ability, since differences were found when L. rhamnosus CRL1505 and the immunomodulatory strain L. rhamnosus IBL027 were compared. This investigation represents a thorough exploration of the role of immunobiotic lactobacilli in improving humoral and cellular adaptive immune responses against IFV in the context of both infection and vaccination.
Copyright ? 2019 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] Adaptive immune response; Immunobiotics; Influenza virus; Lactobacillus rhamnosus CRL1505; Respiratory immunity; Vaccination
PMID: 31841753 DOI: 10.1016/j.intimp.2019.106115
Tonetti FR[SUP]1[/SUP], Islam MA[SUP]2[/SUP], Vizoso-Pinto MG[SUP]1[/SUP], Takahashi H[SUP]3[/SUP], Kitazawa H[SUP]4[/SUP], Villena J[SUP]5[/SUP].
[h=3]Author information[/h] 1 Infection Biology Laboratory, INSIBIO (UNT-CONICET), Tucum?n, Argentina; Laboratory of Basic Sciences - Genetics, Faculty of Medicine, National University of Tucuman, Tucum?n, Argentina. 2 Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan. 3 Laboratory of Plant Pathology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan; Plant Immunology Unit, International Education and Research Center for Food Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan. 4 Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan; Livestock Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai, Japan. Electronic address: haruki.kitazawa.c7@tohoku.ac.jp. 5 Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan; Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucum?n, Argentina. Electronic address: jcvillena@cerela.org.ar.
[h=3]Abstract[/h] The nasal priming with Lactobacillus rhamnosus CRL1505 modulates the respiratory antiviral innate immune response and improves protection against influenza virus (IFV) challenge in mice. However, the potential beneficial effect of the CRL1505 strain on the adaptive immune response triggered by IFV infection or vaccination was not evaluated before. In this work, we demonstrated that nasally administered L. rhamnosus CRL1505 is able to improve both the humoral and cellular adaptive immune responses induced by IFV infection or vaccination. Higher levels of IFV-specific IgA and IgG as well as IFN-γ were found in the serum and the respiratory tract of CRL1505-treated mice after IFV challenge. Lactobacilli treated mice also showed reduced concentrations of IL-17 and improved levels of IL-10 during IFV infection. The differential balance of inflammatory and regulatory cytokines induced by L. rhamnosus CRL1505 contributed to the protection against IFV by favoring an effective effector immune response without inducing inflammatory-mediated lung damage. The optimal immunomodulatory effect of the CRL1505 strain was achieved with viable bacteria. However, non-viable L. rhamnosus CRL1505 was also efficient in improving the adaptive immune responses generated by IFV challenges and therefore, emerged as an interesting alternative for vaccination of immunocompromised hosts. Similar to other immunomodulatory properties of lactobacilli, it was shown here that the adjuvant effect in the context of IFV vaccination was a strain dependent ability, since differences were found when L. rhamnosus CRL1505 and the immunomodulatory strain L. rhamnosus IBL027 were compared. This investigation represents a thorough exploration of the role of immunobiotic lactobacilli in improving humoral and cellular adaptive immune responses against IFV in the context of both infection and vaccination.
Copyright ? 2019 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] Adaptive immune response; Immunobiotics; Influenza virus; Lactobacillus rhamnosus CRL1505; Respiratory immunity; Vaccination
PMID: 31841753 DOI: 10.1016/j.intimp.2019.106115