tetano
Editor, Senior Moderator
Chem Asian J. 2012 Jun 19. doi: 10.1002/asia.201200222. [Epub ahead of print]
Nanowire Transistor-Based Ultrasensitive Virus Detection with Reversible Surface Functionalization.
Chiang PL, Chou TC, Wu TH, Li CC, Liao CD, Lin JY, Tsai MH, Tsai CC, Sun CJ, Wang CH, Fang JM, Chen YT.
Source
Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617 Taiwan (R.O.C), Fax: (+886) 2-2363-6359, Fax: (+886) 2-2363-7812; Institute of Atomic and Molecular Sciences, Academia Sinica, P. O. Box 23-166, Taipei 106, Taiwan (R.O.C).
Abstract
We have applied a reusable silicon nanowire field-effect transistor (SiNW-FET) as a biosensor to conduct ultrasensitive detection of H5N2 avian influenza virus (AIV) in very dilute solution. The reversible surface functionalization of SiNW-FET was made possible using a disulfide linker. In the surface functionalization, 3-mercaptopropyltrimethoxysilane (MPTMS) was first modified on the SiNW-FET (referred to as MPTMS/SiNW-FET), with subsequent dithiothreitol washing to reduce any possible disulfide bonding between the thiol groups of MPTMS. Subsequently, receptor molecules could be immobilized on the MPTMS/SiNW-FET by the formation of a disulfide bond. The success of the reversible surface functionalization was verified with fluorescence examination and electrical measurements. A surface topograph of the SiNW-FET biosensor modified with a monoclonal antibody against H5N2 virus (referred to as mAb(H5) /SiNW-FET) after detecting approximately 10(-17) M H5N2 AIVs was scanned by atomic force microscopy to demonstrate that the SiNW-FET is capable of detecting very few H5N2 AIV particles.
Copyright ? 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PMID:
22715151
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22715151
Nanowire Transistor-Based Ultrasensitive Virus Detection with Reversible Surface Functionalization.
Chiang PL, Chou TC, Wu TH, Li CC, Liao CD, Lin JY, Tsai MH, Tsai CC, Sun CJ, Wang CH, Fang JM, Chen YT.
Source
Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617 Taiwan (R.O.C), Fax: (+886) 2-2363-6359, Fax: (+886) 2-2363-7812; Institute of Atomic and Molecular Sciences, Academia Sinica, P. O. Box 23-166, Taipei 106, Taiwan (R.O.C).
Abstract
We have applied a reusable silicon nanowire field-effect transistor (SiNW-FET) as a biosensor to conduct ultrasensitive detection of H5N2 avian influenza virus (AIV) in very dilute solution. The reversible surface functionalization of SiNW-FET was made possible using a disulfide linker. In the surface functionalization, 3-mercaptopropyltrimethoxysilane (MPTMS) was first modified on the SiNW-FET (referred to as MPTMS/SiNW-FET), with subsequent dithiothreitol washing to reduce any possible disulfide bonding between the thiol groups of MPTMS. Subsequently, receptor molecules could be immobilized on the MPTMS/SiNW-FET by the formation of a disulfide bond. The success of the reversible surface functionalization was verified with fluorescence examination and electrical measurements. A surface topograph of the SiNW-FET biosensor modified with a monoclonal antibody against H5N2 virus (referred to as mAb(H5) /SiNW-FET) after detecting approximately 10(-17) M H5N2 AIVs was scanned by atomic force microscopy to demonstrate that the SiNW-FET is capable of detecting very few H5N2 AIV particles.
Copyright ? 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PMID:
22715151
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22715151