tetano
Editor, Senior Moderator
Nanomedicine
. 2025 Aug 20:102853.
doi: 10.1016/j.nano.2025.102853. Online ahead of print. Universal and highly sensitive detection of influenza A virus and streptococcus pneumoniae using WGA-modified magnetic SERS nanotags-based lateral flow assay
Xiaofei Jia[SUP] 1 [/SUP], Zhenzhen Liu[SUP] 2 [/SUP], Juan Zhou[SUP] 3 [/SUP], Chunran Cao[SUP] 4 [/SUP], Yunwei Hao[SUP] 4 [/SUP], Jin Chen[SUP] 2 [/SUP], Han Han[SUP] 2 [/SUP], Jing Liang[SUP] 2 [/SUP], Zhibin Zhao[SUP] 5 [/SUP], Yi Wang[SUP] 6 [/SUP], Zhendong Niu[SUP] 7 [/SUP], Rui Xiao[SUP] 8 [/SUP]
Affiliations
Infection with influenza A (FluA) virus usually leads to secondary infection with streptococcus pneumoniae (s. pneumoniae). It is urgent to develop rapid, highly sensitive, simultaneous and universal point-of-care testing (POCT) techniques for FluA and s. pneumoniae. We developed a two-channel surface-enhanced Raman scattering (SERS)-lateral flow assay (LFA) technique based on wheat germ agglutinin (WGA)-modified magnetic SERS nanotags (Fe[SUB]3[/SUB]O[SUB]4[/SUB]@Au-WGA), for detection of FluA and s. pneumoniae. The detection limits were 14 copies/mL for FluA and 10 cells/mL for s. pneumoniae, and the sensitivity was about 100 times higher than the visual signals. It was used to detect 80 FluA and s. pneumoniae positive throat swabs/sputum samples and 30 negative samples, with a diagnostic accuracy of 100 %, which was 19 % higher than that of commercial colloidal gold-LFA strips. Therefore, the proposed platform has a strong clinical application potential in the rapid, accurate, highly sensitive and universal detection of FluA and s. pneumoniae.
Keywords: Fe(3)O(4)@Au-WGA nanotags; FluA; Lateral flow assay; S. pneumoniae; Universal detection.
. 2025 Aug 20:102853.
doi: 10.1016/j.nano.2025.102853. Online ahead of print. Universal and highly sensitive detection of influenza A virus and streptococcus pneumoniae using WGA-modified magnetic SERS nanotags-based lateral flow assay
Xiaofei Jia[SUP] 1 [/SUP], Zhenzhen Liu[SUP] 2 [/SUP], Juan Zhou[SUP] 3 [/SUP], Chunran Cao[SUP] 4 [/SUP], Yunwei Hao[SUP] 4 [/SUP], Jin Chen[SUP] 2 [/SUP], Han Han[SUP] 2 [/SUP], Jing Liang[SUP] 2 [/SUP], Zhibin Zhao[SUP] 5 [/SUP], Yi Wang[SUP] 6 [/SUP], Zhendong Niu[SUP] 7 [/SUP], Rui Xiao[SUP] 8 [/SUP]
Affiliations
- PMID: 40846168
- DOI: 10.1016/j.nano.2025.102853
Infection with influenza A (FluA) virus usually leads to secondary infection with streptococcus pneumoniae (s. pneumoniae). It is urgent to develop rapid, highly sensitive, simultaneous and universal point-of-care testing (POCT) techniques for FluA and s. pneumoniae. We developed a two-channel surface-enhanced Raman scattering (SERS)-lateral flow assay (LFA) technique based on wheat germ agglutinin (WGA)-modified magnetic SERS nanotags (Fe[SUB]3[/SUB]O[SUB]4[/SUB]@Au-WGA), for detection of FluA and s. pneumoniae. The detection limits were 14 copies/mL for FluA and 10 cells/mL for s. pneumoniae, and the sensitivity was about 100 times higher than the visual signals. It was used to detect 80 FluA and s. pneumoniae positive throat swabs/sputum samples and 30 negative samples, with a diagnostic accuracy of 100 %, which was 19 % higher than that of commercial colloidal gold-LFA strips. Therefore, the proposed platform has a strong clinical application potential in the rapid, accurate, highly sensitive and universal detection of FluA and s. pneumoniae.
Keywords: Fe(3)O(4)@Au-WGA nanotags; FluA; Lateral flow assay; S. pneumoniae; Universal detection.