tetano
Editor, Senior Moderator
Nanomedicine
. 2021 Sep 25;102463.
doi: 10.1016/j.nano.2021.102463. Online ahead of print.
Nanoparticles based on artificial self-assembling peptide and displaying M2e peptide and stalk HA epitopes of influenza a virus induces potent humoral and T-cell responses and protects against the viral infection
Anna A Zykova[SUP] 1 [/SUP], Elena A Blokhina[SUP] 1 [/SUP], Liudmila A Stepanova[SUP] 2 [/SUP], Marina A Shuklina[SUP] 2 [/SUP], Liudmila M Tsybalova[SUP] 2 [/SUP], Victor V Kuprianov[SUP] 1 [/SUP], Nikolai V Ravin[SUP] 3 [/SUP]
Affiliations
Abstract
The extracellular domain of the M2 protein (M2e) and conserved region of the second subunit of the hemagglutinin (HA2) could be used for the development of broad-spectrum vaccines against influenza A. Here we obtained and characterized recombinant mosaic proteins containing tandem copies of M2e and HA2 fused to an artificial self-assembling peptide (SAP). The inclusion of SAP peptides in the fusion proteins enabled their self-assembly in vitro into spherical particles with a size of 30-50nm. Intranasal immunization of mice with these particles without additional adjuvants induced strong humoral immune response against M2e and the whole virus. Particles carrying both M2e and HA2 induced antigen-specific multifunctional CD4+ effector memory T cells. Immunization provided high protection of mice against the lethal challenge with different subtypes of influenza A virus. The obtained self-assembling nanoparticles can be used to develop a universal influenza vaccine.
Keywords: Hemagglutinin; Influenza; M2e peptide; Nanoparticle; Recombinant vaccine.
. 2021 Sep 25;102463.
doi: 10.1016/j.nano.2021.102463. Online ahead of print.
Nanoparticles based on artificial self-assembling peptide and displaying M2e peptide and stalk HA epitopes of influenza a virus induces potent humoral and T-cell responses and protects against the viral infection
Anna A Zykova[SUP] 1 [/SUP], Elena A Blokhina[SUP] 1 [/SUP], Liudmila A Stepanova[SUP] 2 [/SUP], Marina A Shuklina[SUP] 2 [/SUP], Liudmila M Tsybalova[SUP] 2 [/SUP], Victor V Kuprianov[SUP] 1 [/SUP], Nikolai V Ravin[SUP] 3 [/SUP]
Affiliations
- PMID: 34583058
- DOI: 10.1016/j.nano.2021.102463
Abstract
The extracellular domain of the M2 protein (M2e) and conserved region of the second subunit of the hemagglutinin (HA2) could be used for the development of broad-spectrum vaccines against influenza A. Here we obtained and characterized recombinant mosaic proteins containing tandem copies of M2e and HA2 fused to an artificial self-assembling peptide (SAP). The inclusion of SAP peptides in the fusion proteins enabled their self-assembly in vitro into spherical particles with a size of 30-50nm. Intranasal immunization of mice with these particles without additional adjuvants induced strong humoral immune response against M2e and the whole virus. Particles carrying both M2e and HA2 induced antigen-specific multifunctional CD4+ effector memory T cells. Immunization provided high protection of mice against the lethal challenge with different subtypes of influenza A virus. The obtained self-assembling nanoparticles can be used to develop a universal influenza vaccine.
Keywords: Hemagglutinin; Influenza; M2e peptide; Nanoparticle; Recombinant vaccine.