tetano
Editor, Senior Moderator
Nanomaterials (Basel)
. 2022 Jan 17;12(2):284.
doi: 10.3390/nano12020284.
Movable Layer Device for Rapid Detection of Influenza a H1N1 Virus Using Highly Bright Multi-Quantum Dot-Embedded Particles and Magnetic Beads
Islam Seder[SUP] 1 [/SUP], Ahla Jo[SUP] 2 [/SUP], Bong-Hyun Jun[SUP] 2 [/SUP], Sung-Jin Kim[SUP] 1 [/SUP]
Affiliations
Abstract
Preventing the rapid spread of viral infectious diseases has become a major concern for global health. In this study, we present a microfluidic platform that performs an immunoassay of viral antigens in a simple, automated, yet highly sensitive manner. The device uses silica particles embedded with highly bright quantum dots (QD[SUP]2[/SUP]) and performs the immunoassay with a vertically movable top layer and a rotating bottom layer. Through the motion of the layers and the surface tension in the liquids, reagents move from top chambers to bottom chambers and mix homogeneously. A tip in the top layer with a mobile permanent magnet moves the immune complexes comprising the magnetic beads, virus particles, and QD[SUP]2[/SUP] between the bottom chambers. In this way, our automated device achieves a highly sensitive magnetic bead-based sandwich immunoassay for the influenza A H1N1 virus within 32.5 min. The detection limit of our method is 5.1 × 10[SUP]-4[/SUP] hemagglutination units, which is 2 × 10[SUP]3[/SUP] times more sensitive than that of the conventional hemagglutination method and is comparable to PCR. Our device is useful for the rapid and sensitive detection of infectious diseases in point-of-care applications and resource-limited environments.
Keywords: immunoassay; magnetic bead; microfluidics; quantum dot.
. 2022 Jan 17;12(2):284.
doi: 10.3390/nano12020284.
Movable Layer Device for Rapid Detection of Influenza a H1N1 Virus Using Highly Bright Multi-Quantum Dot-Embedded Particles and Magnetic Beads
Islam Seder[SUP] 1 [/SUP], Ahla Jo[SUP] 2 [/SUP], Bong-Hyun Jun[SUP] 2 [/SUP], Sung-Jin Kim[SUP] 1 [/SUP]
Affiliations
- PMID: 35055303
- DOI: 10.3390/nano12020284
Abstract
Preventing the rapid spread of viral infectious diseases has become a major concern for global health. In this study, we present a microfluidic platform that performs an immunoassay of viral antigens in a simple, automated, yet highly sensitive manner. The device uses silica particles embedded with highly bright quantum dots (QD[SUP]2[/SUP]) and performs the immunoassay with a vertically movable top layer and a rotating bottom layer. Through the motion of the layers and the surface tension in the liquids, reagents move from top chambers to bottom chambers and mix homogeneously. A tip in the top layer with a mobile permanent magnet moves the immune complexes comprising the magnetic beads, virus particles, and QD[SUP]2[/SUP] between the bottom chambers. In this way, our automated device achieves a highly sensitive magnetic bead-based sandwich immunoassay for the influenza A H1N1 virus within 32.5 min. The detection limit of our method is 5.1 × 10[SUP]-4[/SUP] hemagglutination units, which is 2 × 10[SUP]3[/SUP] times more sensitive than that of the conventional hemagglutination method and is comparable to PCR. Our device is useful for the rapid and sensitive detection of infectious diseases in point-of-care applications and resource-limited environments.
Keywords: immunoassay; magnetic bead; microfluidics; quantum dot.