<table class="searchform" summary="layout" align="center" border="0" cellpadding="5" cellspacing="0" width="98%"><tbody><tr><th class="heading" align="right" valign="top">Title</th> <td class="shade-light" valign="top">Mutant frequency on the hprt gene in T-lymphocytes from Brazilian children accidentally exposed to cesium-137 ionizing radiation and controls</td></tr> <tr><th class="heading" align="right" valign="top">Creator/Author</th> <td class="shade-white" valign="top">
Saddi, V. ;
Nohturfft, A. ;
Kusser, W.C. ;
Glickman, B.W. [Univ. of Victoria (Canada)] </td></tr> <tr><th class="heading" align="right" nowrap="nowrap" valign="top">Publication Date</th><td class="shade-light" valign="top">1994 Dec 31</td></tr> <tr><th class="heading" align="right" nowrap="nowrap" valign="top">OSTI Identifier</th><td class="shade-white" valign="top">OSTI ID: 88912</td></tr> <tr><th class="heading" align="right" nowrap="nowrap" valign="top">Report Number(s)</th><td class="shade-light" valign="top">CONF-9405324--</td></tr> <tr><th class="heading" align="right" nowrap="nowrap" valign="top">Other Number(s)</th><td class="shade-white" valign="top">Journal ID: EMMUEG; ISSN 0893-6692; TRN: TRN: 95:004220-0057</td></tr> <tr><th class="heading" align="right" nowrap="nowrap" valign="top">Resource Type</th><td class="shade-light" valign="top">Journal Article</td></tr> <tr><th class="heading" align="right" nowrap="nowrap" valign="top">Resource Relation</th><td class="shade-white" valign="top">Journal Name:
Environmental and Molecular Mutagenesis; Journal Volume: 23; Journal Issue: Suppl.23; Conference: 25. annual meeting of the Environmental Mutagen Society, Portland, OR (United States), 7-12 May 1994; Other Information: PBD: 1994</td></tr> <tr><th class="heading" align="right" nowrap="nowrap" valign="top">Subject</th><td class="shade-light" valign="top">56 BIOLOGY AND MEDICINE, APPLIED STUDIES; CHILDREN; GENETIC RADIATION EFFECTS; CESIUM 137; RADIATION DOSES; HUMAN CHROMOSOMES; MUTATIONS; BRAZIL; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE</td></tr> <tr><th class="heading" align="right" nowrap="nowrap" valign="top">Description/Abstract</th><td class="shade-white" valign="top">Blood samples collected from exposed and non-exposed children in Gioiania, Brazil, two years after a radioactive accident involving Cs{sup 137} are analyzed on the basis of their mutant frequency in the hprt gene of T-lymphocytes. The exposed-group consists of ten children (six males and four females) ranging in age from 06 to 14 years. The control-group consists of eight healthy brazilian children (four males and four females) not exposed to Cs{sup 137}, ranging in age form 04 to 16 years. The hprt-cloning assay was the chosen method for this study. Hprt mutant frequency was determined for both groups of children. The mean mutant frequency for the control group was 3.85 x 10-{sup 6}, and for the exposed group a mean of 18.05 x 10-{sup 6}. The mean mutant frequency detected for the control group does not differ meaningfully from the few data available about mutant frequency in children.
However, for the exposed group the mean value was five-fold higher. In some cases the mutant frequency in the exposed group seems higher than might have been expected from the estimated exposure. In these cases the T-cell receptor gene rearrangement assay must be performed in order to clarify, or to correct the observed mutant frequency.</td></tr></tbody></table>
